Abstract
The adsorption energy distributions (AED) of a few neutral and ionic compounds were determined onto Gemini-C18, an endcapped silica-C18 surface, from solutions of methanol and water. The adsorption data were accurately measured using the dynamic frontal analysis method. The adsorption heterogeneity of this surface toward neutral and ionic compounds was investigated by calculating the AED using the Expectation-Maximization algorithm. The calculations converged toward a well-defined AED in all cases. Neutral compounds adsorb on two distinct types of sites (with relatively low adsorption energies) while the ionic compounds adsorb on three distinct types of sites (two having a weak and one a strong adsorption energy). The combination of these results allows the selection of a satisfactory adsorption isotherm model and the correct prediction of the overloaded band profiles of these compounds, including those of the acido-basic compounds when eluted with a buffer having a weak capacity, e.g. when the ratio of the concentrations of the neutral and ionic conjugated species in the band and the mobile phase pH change during elution.
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Dedicated to the memory of A.V. Kiselev, an outstanding scientist, a wonderful person, a man of great erudition, and a remarkable teacher in the field of adsorption
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Gritti, F., Guiochon, G. Determination of the adsorption energy distribution of neutral and charged compouds onto endcapped silica-C18 adsorbent from polar liquid phases. Colloid J 71, 480–486 (2009). https://doi.org/10.1134/S1061933X09040085
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DOI: https://doi.org/10.1134/S1061933X09040085