Abstract
The purpose of this study was to investigate the optical properties, absorption coefficient (μ a ) scattering coefficient (μ s ) and refractive indices, (n) of HeLa cell line in a suspension of 2% minimum essential medium (MEM) at two different (632.8 and 532.0 nm) wave lengths of laser light. Optical properties were determined with Kubelka Munk Model (KMM) and refractive index measurement was made through minimum angle of deviation method (MAD). We reported μ a = 8.643 ± 0.187 and 2.348 ± 0.249 cm−1 and μ s = 5.609 ± 0.287 and 88.166 ± 2.833 cm−1 at 632.8 and 532.0 nm, respectively. Refractive index was found to be 1.332 and 1.312 at 632.8 nm and 532.0 nm, respectively. The discussed results provide a route of information for clinical diagnosis, therapeutic application and dosimetry studies in HeLa and other cell lines.
Similar content being viewed by others
References
A. N. Bashkatov, E. A. Genina, V. I. Kochubey, and V. V. Tuchin, Appl. Phys. 38, 2543 (2005).
J. Ferreira, L. T. Moriyama, C. Kurachi, et al., Laser Phys. Lett. 4, 469 (2007).
M. Ikram, R. Khan, S. Firdous, et al., Laser Phys. 21, 427 (2011).
M. Atif, M. Fakhar-e-Alam, S. Zaidi, and R. Suleman, Laser Phys. 21, 1135 (2011).
M. Fakhar-e-Alam, M. Atif, M. AlSalhi, et al., Laser Phys. 21, 972 (2011).
L. G. Sabino, L. M. V. de Negreiros, J. D. Vollet-Filho, et al., Laser Phys. Lett. 8, 239 (2011).
C. S. Xu and A. W. N. Leung, Laser Phys. Lett. 7, 68 (2010).
L. Buriankova, Z. Nadova, D. Jancura, et al., Laser Phys. Lett. 7, 613 (2010).
Y. Shen, H. Y. Lin, Z. F. Huang, et al., Phys. Lett. 8, 232 (2011).
C. Dressler, D. Schwandt, J. Beuthan, et al., Laser Phys. Lett. 7, 817 (2010).
J. T. Bruulsema, J. E. Hayward, T. J. Farrell, et al., Opt. Lett. 22, 190 (1997).
E. Salomatina, B. Jiang, J. Novak, and A. N. Yaroslavsky, Biomedical. Opt. 11, 064026 (2006).
U. Hafeez, M. Atif, S. Firdous, et al., Opt. Spect. 110, 313 (2011).
H. Ullah, M. Atif, S. Firdous, et al., Laser Phys. Lett. 7, 889 (2010).
D. K. Sardar and L. B. Levy, Lasers Med. Sci. 13, 106 (1998).
D. K. Sardar, B. G. Yust, F. J. Barrera, et al., Lasers Med. Sci. 24, 839 (2009).
W. F. Cheong, S. A. Prahl, and A. J. Welch, IEEE Qunatam Elect. 26, 2166 (1990).
H. J. Wei, D. Xing, G. Y. Wu, et al., World J. Gst. 9, 2068 (2003).
H. J. Wei, D. Xing, J. J. Lu, et al., World J. Gst. 11, 2413 (2005).
M. Atif, S. Firdous, and M. Nawaz, Lasers Med. Sci. 25, 545 (2010).
R. Tchao and A. B. Schleich, Cell Bio. Int. Rpot. 2, 353 (1978).
B. S. Jacobson and U. S. Ryan, Tissue Cell. 14, 69 (1982).
Brunsting, A. Mullaney, and F. Paul, Biophys. J. 14, 439 (1974).
N. Lue, G. Popescu, T. Ikeda, et al., Opt. Lett. 31, 2759 (2006).
R. K. Bista, S. Uttam, P. Wang, et al., Biomed. Opt. 16, 070503 (2011).
X. J. Liang, A. Q. Liu, C. S. Lim, et al., Sensors Act., Ser. A 133, 349 (2007).
G. E. Palade, Anat. Rec. 114, 427 (1952).
V. V. Tuchin, Phys. Usp. 40, 495 (1997).
J. R. Zijp and J. J. ten Bosch, Archives Oral Bio. 36, 283 (1991).
R. Marchesini, A. Bertoni, S. Andreola, et al., Appl. Opt. 28, 2318 (1989).
M. Atif, M. Fakhar-e-Alam, L. G. Sabino, et al., Laser Phys. Lett. 8, 386 (2011).
C. S. Xu, A. W. N. Leung, L. Liu, and X. S. Xia, Laser Phys. Lett. 7, 544 (2010).
E. S. Tuchina and V. V. Tuchin, Laser Phys. Lett. 7, 607 (2010).
M. Atif, S. Firdous, A. Khurshid, et al., Laser Phys. Lett. 6, 886 (2009).
M. M. Tagliani, C. F. Oliveira, E. M. M. Lins, et al., Laser Phys. Lett. 7, 247 (2010).
S. Pop, R. Ion, M. Neagu, and C. Constantin, Laser Phys. 21, 576 (2011).
M. Atif, M. Fakhar-e-Alam, S. Firdous, et al., Laser Phys. Lett. 7, 757 (2010).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Text © Astro, Ltd., 2012.
The article is published in the original.
Rights and permissions
About this article
Cite this article
Rehman, A., Firdous, S., Nawaz, M. et al. Optical parameters measurement for diagnostic and photodynamic therapy of human cervical adenocarcinoma (HeLa) cell line. Laser Phys. 22, 322–326 (2012). https://doi.org/10.1134/S1054660X12010161
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1054660X12010161