Studies have shown that the increase of cell metabolism depends on the low level laser therapy (LLLT) parameters used to irradiate the cells. However, the optimal laser dose to up-regulate pulp cell activity remains unknown. Consequently, the aim of this study was to evaluate the metabolic response of odontoblast-like cells (MDPC-23) exposed to different LLLT doses. Cells at 20000 cells/cm2 were seeded in 24-well plates using plain culture medium (DMEM) and were incubated in a humidified incubator with 5% CO2 at 37°C. After 24 h, the culture medium was replaced by fresh DMEM supplemented with 5% (stress by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to different laser doses from a near infrared diode laser prototype designed to provide a uniform irradiation of the wells. The experimental groups were: G1: 1.5 J/cm2 + 5% FBS; G2: 1.5 J/cm2 + 10% FBS; G3: 5 J/cm2 + 5% FBS; G4: 5 J/cm2 + 10% FBS; G5: 19 J/cm2 + 5% FBS; G6: 19 J/cm2 + 10% FBS. LLLT was performed in 3 consecutive irradiation cycles with a 24-hour interval. Non-irradiated cells cultured in DMEM supplemented with either 5 or 10% FBS served as control groups. The analysis of the metabolic response was performed by the MTT assay 3 h after the last irradiation. G1 presented an increase in SDH enzyme activity and differed significantly (Mann-Whitney test, p < 0.05) from the other groups. Analysis by scanning electron microscopy showed normal cell morphology in all groups. Under the tested conditions, LLLT stimulated the metabolic activity of MDPC-23 cultured in DMEM supplemented with 5% FBS and exposed to a laser dose of 1.5 J/cm2. These findings are relevant for further studies on the action of near infrared lasers on cells with odontoblast phenotype.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
A. Antunes, V. L. R. Salvador, M. A. Scapin, W. de Rossi, and D. M. Zezell, Laser Phys. Lett. 2, 318 (2005).
D. A. M. P. Malta, M. A. M. Kreidler, G. E. Villa, M. F. de Andrade, C. R. Fontana, and R. F. Z. Lizarelli, Laser Phys. Lett. 4, 153 (2007).
H. Jelínkova, T. Dostálová, M. N mec, P. Koranda, P. Šim nek, M. Miyagi, Y.-W. Shi, and Y. Matsuura, Laser Phys. Lett. 3, 43 (2006).
R. de F. Z. Lizarelli, L. T. Moriyama, J. R. P. Jorge, and V. S. Bagnato, Laser Phys. 16, 849 (2006).
H. JelÍnkova, J. Šulc, T. Dostálová, P. Koranda, M. Němec, and P. Hofmanova, Laser Phys. Lett. 6, 222 (2009).
M. Khadra, S. P. Lyngstadaas, H. R. Haanaes, and K. Mustafa, J. Biomed. Mater. Res. 73, 55 (2005).
S. Parker, Br. Dent. J. 202, 131 (2007).
T. Karu, J. Photochem. Photobiol. B 49, 1 (1999).
T. Karu, L. Pyatibrat, and G. Kalendo, J. Photochem. Photobiol. 27, 219 (1995).
H. Kikuchi, T. Sawda, and T. Yanagisawa, Arch. Oral Biol. 41, 871 (1996).
T. Karu, L. V. Pyatibrat, G. S. Kalendo, and R. O. Esenaliev, Lasers Surg. Med. 18, 171 (1996).
M. Koutná, R. Janisch, and R. Veselska, Scr. Med. (BRNO) 76, 163 (2003).
J. L. N. Bastos, R. F. Z. Lizarelli, and N. A. Parizotto, Laser Phys. 19, 1925 (2009).
R. A. Prates, E. G. da Silva, A. M. Yamada, Jr., L. C. Suzuki, C. R. Paula, and M. S. Ribeiro, Laser Phys. 19, 1038 (2009).
A. R. Coombe, C. T. G. Ho, and M. A. Darendeliler, Clin. Orthod. 4, 3 (2001).
L. Almeida-Lopes, J. Rigau, R. A. Zangaro, J. Guidugli-Neto, and M. M. M. Jaeger, Lasers Surg. Med. 29, 179 (2001).
N. Grossman, N. Schneid, H. Reuveni, S. Halevy, and R. Lubart, Lasers Surg. Med. 22, 212 (1998).
M. Khadra, P. S. Lyngstadaas, R. H. Haanæs, and K. Mustafa, Biomaterials 26, 3503 (2005).
M. Khadra, N. Kasem, S. P. Lyngstadaas, H. R. Haanses, and K. Mustafa, Clin. Oral Implants Res. 16, 168 (2005).
P. Moore, T. D. Ridgway, R. G. Higbee, E. W. Howard, and M. D. Lucroy, Lasers Surg. Med. 36, 8 (2005).
N. Aihara, M. Yamaguchi, and K. Kasai, Lasers Med. Sci. 21, 24 (2006).
K. Yamada, J. Jpn. Orthop. Assoc. 65, 787 (1991).
S. Hamajima, K. Hiratsuka, M. Kiyama-Kishikawa, T. Tagama, M. Kawahara, M. Ohta, H. Sasahara, and Y. Abiko, Lasers Med. Sci. 18, 78 (2003).
A. Stein, D. Benayahu, D. Maltz, and U. Oron, Photomed. Laser Surg. 23, 161 (2005).
Y. Kimura, P. Wilder-Smith, K. Yonaga, and K. Matsumoto, J. Clin. Periodontol. 27, 715 (2000).
F. Schwarz, N. Arweiler, T. Georg, and E. Reich, J. Clin. Periodontol. 29, 211 (2002).
T. M. Ciaramicoli, C. R. R. Carvalho, and P. C. Eduardo, Lasers Surg. Med. 33, 358 (2003).
E. M. A. Clavijo, V. R. G. Clavijo, M. C. Bandeca, M. R. Nadalin, M. F. Andrade, J. R. C. Saad, and A. A. Pelegrine, Laser Phys. 19, 2041 (2009).
R. H. Dababneh, A. T. Khouri, and M. Addy, Br. Dent. J. 187, 606 (1999).
G. E. P. Villa, A. B. C. E. D. Catirse, R. C. C. Lia, and R. F. Z. Lizarelli, Laser Phys. Lett. 4, 690 (2007).
C. F. Oliveira, J. Hebling, P. P. C. Souza, N. T. Sacono, F. R. Lessa, R. F. Z. Lizarelli, and C. A. S. Costa, Laser Phys Lett. 5, 680 (2008).
E. M. V. B. Cagnie, M. J. Cornelissen, H. A. Declerecq, and D. C. Cambier, Lasers Surg. Med. 18, 95 (2003).
Y. L. Jia and Z. Y. Guo, Lasers Surg. Med. 34, 323 (2004).
M. Kreisler, A. B. Christoffer, B. Willerstausen, and B. d’Hoedt, J. Clin. Periodontol. 30, 353 (2003).
T. Mosmann, J. Immunol. Methods 65, 55 (1983).
S. A. Gonchukov and Yu. B. Lazarev, Laser Phys. 13, 749 (2003).
H. Tuby, L. Maltz, and U. Oron, Lasers Surg. Med. 39, 373 (2007).
N. Mochizuki-Oda, Y. Kataoka, Y. Cui, H. Yamada, M. Heya, and K. Awazu, Neurosci. Lett. 323, 207 (2002).
H. Kale, P. Harikuman, S. B. Kulkarni, P. M. Nair, and M. S. Netrawali, Mutat. Res. 298, 17 (1992).
G. Y. Fraikin, M. G. Strakhovskaya, and A. B. Rubin, J. Photochem. Photobiol. B 34, 129 (1996).
A. M. Edwards and E. Silva, J. Photochem. Photobiol. B 63, 126 (2001).
T. Karu, Lasers Life Sci. 2, 52 (1988).
T. I. Karu, L. V. Pyatibrat, and G. S. Kalendo, Photochem. Photobiol. Sci. 3, 211 (2004).
R. Lubart, M. Eichler, R. Lavi, H. Friedman, and A. Shainberg, Photomed. Laser Surg. 23, 3 (2005).
M. M. Marques, A. N. Pereira, N. A. Fujihara, F. N. Nogueira, and C. P. Eduardo, Lasers Surg. Med. 34, 260 (2004).
S. N. Pereira, C. P. Eduardo, E. Matson, and M. M. Marques, Lasers Surg. Med. 31, 263 (2002).
T. Karu, J. Photochem. Photobiol. 52, 1089 (1990).
Original Text © Astro, Ltd., 2010.
The article is published in the original.
Rights and permissions
About this article
Cite this article
Oliveira, C.F., Basso, F.G., Lins, E.C. et al. Increased viability of odontoblast-like cells subjected to low-level laser irradiation. Laser Phys. 20, 1659–1666 (2010). https://doi.org/10.1134/S1054660X10130153
- Fetal Bovine Serum
- Laser Phys
- Laser Physics
- Irradiate Group
- Dentin Hypersensitivity