Skip to main content

Analysis of the penetration of a caffeine containing shampoo into the hair follicles by in vivo laser scanning microscopy

Abstract

In previous in vitro investigations, it was demonstrated that caffeine is able to stimulate the hair growth. Therefore, a penetration of caffeine into the hair follicle is necessary. In the present study, in vivo laser scanning microscopy (LSM) was used to investigate the penetration and storage of a caffeine containing shampoo into the hair follicles. It was shown that a 2-min contact time of the shampoo with the skin was enough to accumulate significant parts of the shampoo in the hair follicles. A penetration of the shampoo up to a depth of approx. 200 μm could be detected, which represents the detection limit of the LSM. At this depth, the close network of the blood capillaries surrounding the hair follicles commences. Even after 24 h, the substance was still detectable in the hair follicles. This demonstrates the long-term reservoir function of the hair follicles for topically applied substances such as caffeine.

This is a preview of subscription content, access via your institution.

References

  1. D. A. Godwin and B. B. Michniak, Drug Dev. Ind. Pharm. 25, 905 (1999).

    Article  Google Scholar 

  2. A. Rougier, C. Lotte, and H. I. Maibach, J Pharm Sci. 76, 451 (1987).

    Article  Google Scholar 

  3. G. K. Menon and A. M. Kligman, Skin Pharmacol. Physiol. 22, 178 (2009).

    Article  Google Scholar 

  4. S. Wiedersberg, C. S. Leopold, and R. H. Guy, Skin Pharmacol. Physiol 22, 128 (2009).

    Article  Google Scholar 

  5. N. Otberg, A. Teichmann, U. Rasuljev, R. Sinkgraven, W. Sterry, and J. Lademann, Skin Pharmacol. Physiol. 20, 195 (2007).

    Article  Google Scholar 

  6. N. Otberg, A. Patzelt, U. Rasulev, T. Hagemeister, M. Linscheid, R. Sinkgraven, W. Sterry, and J. Lademann, Br. J. Clin. Pharmacol. 65, 488 (2008).

    Article  Google Scholar 

  7. A. Teichmann, H. Richter, F. Knorr, C. Antoniou, W. Sterry, and J. Lademann, Laser Phys. Lett. 4, 464 (2007).

    Article  Google Scholar 

  8. U. Jacobi, R. Toll, W. Sterry, and J. Lademann, Laser Phys. 15, 1594 (2005).

    Google Scholar 

  9. M. Ossadnik, H. Richter, A. Teichmann, S. Koch, U. Schafer, R. Wepf, W. Sterry, and J. Lademann, Laser Phys. 16, 747 (2006).

    Article  ADS  Google Scholar 

  10. N. Otberg, H. Richter, H. Schaefer, U. Blume-Peytavi, W. Sterry, and J. Lademann, Laser Phys. 13, 761 (2003).

    Google Scholar 

  11. S. I. Yum and J. Roe, Diabetes Technol. Ther. 1, 29 (1999).

    Article  Google Scholar 

  12. C. Borelli, J. Gassmueller, J. W. Fluhr, K. H. Nietsch, S. Schinzel, and H. C. Korting, Skin Pharmacol. Physiol. 21, 181 (2008).

    Article  Google Scholar 

  13. J. W. Fluhr, R. Darlenski, I. Angelova-Fischer, N. Tsankov, and D. Basketter, Skin Pharmacol. Physiol. 21, 124 (2008).

    Article  Google Scholar 

  14. N. Otberg, H. Richter, H. Schaefer, U. Blume-Peytavi, W. Sterry, and J. Lademann, J. Invest. Dermatol. 122, 14 (2004).

    Article  Google Scholar 

  15. A. P. Popov, A. V. Priezzhev, J. Lademann, and R. Myllyla, Laser Phys. 16, 751 (2006).

    Article  ADS  Google Scholar 

  16. J. Lademann, F. Knorr, H. Richter, U. Blume-Peytavi, A. Vogt, C. Antoniou, W. Sterry, and A. Patzelt, Skin Pharmacol. Physiol. 21, 150 (2008).

    Article  Google Scholar 

  17. K. Abdulmajed and C. M. Heard, Skin Pharmacol. Physiol. 21, 46 (2008).

    Article  Google Scholar 

  18. J. Lademann, N. Otberg, U. Jacobi, R. M. Hoffman, and U. Blume-Peytavi, J. Investig. Dermatol. Symp. Proc. 10, 301 (2005).

    Article  Google Scholar 

  19. J. Lademann, H. Richter, U. F. Schaefer, U. Blume- Peytavi, A. Teichmann, N. Otberg, and W. Sterry, Skin Pharmacol. Physiol. 19, 232 (2006).

    Article  Google Scholar 

  20. U. Jacobi, E. Waibler, W. Sterry, and J. Lademann, Laser Phys. 15, 565 (2005).

    Google Scholar 

  21. Y. P. Lu, Y. R. Lou, J. Liao, J. G. Xie, Q. Y. Peng, C. S. Yang, and A. H. Conney, Carcinogenesis 26, 1465 (2005).

    Article  Google Scholar 

  22. S. C. Wilkinson, W. J. Maas, J. B. Nielsen, L. C. Greaves, J. J. van de Sandt, and F. M. Williams, Int. Arch. Occup. Environ. Health 79, 405 (2006).

    Article  Google Scholar 

  23. M. E. Darvin, I. Gersonde, H. Albrecht, S. A. Gonchukov, W. Sterry, and J. Lademann, Laser Phys. 15, 295 (2005).

    Google Scholar 

  24. M. E. Darvin, I. Gersonde, H. Albrecht, W. Sterry, and J. Lademann, Laser Phys. 16, 833 (2006).

    Article  ADS  Google Scholar 

  25. M. E. Darvin, I. Gersonde, S. Ey, N. N. Brandt, H. Albrecht, S. A. Gonchukov, W. Sterry, and J. Lademann, Laser Phys. 14, 231 (2004).

    Google Scholar 

  26. J. G. Dorea and T. H. da Costa, Br. J. Nutr. 93, 773 (2005).

    Article  Google Scholar 

  27. J. Lademann, P. J. Caspers, A. van der Pol, H. Richter, A. Patzelt, L. Zastrow, M. Darvin, W. Sterry, and J. W. Fluhr, Laser Phys. Lett. 6, 76 (2009).

    Article  Google Scholar 

  28. T. W. Fischer, U. C. Hipler, and P. Elsner, Int. J. Dermatol. 46, 27 (2007).

    Article  Google Scholar 

  29. J. Lademann, A. Patzelt, M. Worm, H. Richter, W. Sterry, and M. Meinke, Laser Phys. Lett. 6, 759 (2009).

    Article  Google Scholar 

  30. J. Lademann, H. Richter, N. Otberg, F. Lawrenz, U. Blume-Peytavi, and W. Sterry, Laser Phys. 13, 756 (2003).

    Google Scholar 

  31. L. Meyer, N. Otberg, H. Richter, W. Sterry, and J. Lademann, Laser Phys. 16, 758 (2006).

    Article  ADS  Google Scholar 

  32. C. Antoniou, J. Lademann, H. Richter, S. Astner, A. Patzelt, L. Zastrow, W. Sterry, and S. Koch, Laser Phys. Lett. 6, 393 (2009).

    Article  Google Scholar 

  33. J. Lademann, J. Shevtsova, A. Patzelt, H. Richter, N. D. Gladkowa, V. M. Gelikonov, S. A. Gonchukov, W. Sterry, and U. Blume-Peytavi, Laser Phys. Lett. 5, 908 (2008).

    Article  Google Scholar 

  34. S. Dietterle, J. Lademann, H. J. Rowert-Huber, E. Stockfleth, C. Antoniou, W. Sterry, and S. Astner, Laser Phys. Lett. 5, 752 (2008).

    Article  Google Scholar 

  35. J. Lademann, S. Schanzer, H. Richter, C. Antoniou, F. Knorr, W. Sterry, and A. Patzelt, Laser Phys. Lett. 5, 686 (2008).

    Article  Google Scholar 

  36. J. Lademann, A. Patzelt, M. Darvin, H. Richter, C. Antoniou, W. Sterry, and S. Koch, Laser Phys. Lett. 5, 335 (2008).

    Article  Google Scholar 

  37. J. Lademann, H. Richter, S. Astner, A. Patzelt, F. Knorr, W. Sterry, and C. Antoniou, Laser Phys. Lett. 5, 311 (2008).

    Article  Google Scholar 

  38. U. Jacobi, T. Tassopoulos, C. Surber, and J. Lademann, Arch. Dermatol. Res. 297, 303 (2006).

    Article  Google Scholar 

  39. H. Kandarova, H. Richter, M. Liebsch, and J. Lademann, Laser Phys. Lett. 4, 308 (2007).

    Article  Google Scholar 

  40. A. Martschick, A. Teichmann, H. Richter, S. Schanzer, C. Antoniou, W. Sterry, and J. Lademann, Laser Phys. Lett. 4, 395 (2007).

    Article  Google Scholar 

  41. L. Meyer, N. Otberg, H. J. Tietz, W. Sterry, and J. Lademann, Laser Phys. Lett. 2, 148 (2005).

    Article  Google Scholar 

  42. U. Jacobi, E. Waibler, J. Bartoll, P. Schulze, W. Sterry, and J. Lademann, Laser Phys. Lett. 1, 100 (2004).

    Article  Google Scholar 

  43. T. Rieger, A. Teichmann, H. Richter, W. Sterry, and J. Lademann, Laser Phys. Lett. 4, 72 (2007).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to J. Lademann.

Additional information

Original Russian Text © Astro, Ltd., 2010.

The article is published in the original.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lademann, J., Richter, H., Schanzer, S. et al. Analysis of the penetration of a caffeine containing shampoo into the hair follicles by in vivo laser scanning microscopy. Laser Phys. 20, 551–556 (2010). https://doi.org/10.1134/S1054660X10030102

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1054660X10030102

Keywords

  • Caffeine
  • Stratum Corneum
  • Hair Follicle
  • Skin Surface
  • Laser Phys