Abstract
The true molecular photophysical parameters’ values of tryptophan residues in single-tryptophan-containing and two-tryptophan-containing proteins (by the example of human serum albumin and bovine serum albumin) have been determined for the first time with the use of the authors’ suggested algorithm. The algorthm is based on the simultaneous use of nonlinear and kinetic-laser fluorimetry. The obtained result opens up new approaches in the monitoring of living systems and other objects, containing single fluorophores and localized pairs of fluorophores.
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References
E. A. Permyakov, The Method of Intrinsic Protein Luminescence (Nauka, Moscow, 2003), p. 72 [in Russian].
I. M. Kuznecova and K. K. Turoverov, Tsitologiya 40, 747 (1998).
A.K. Gaigalas, K.D. Cole, S. Bykadi, and Paul DeRose, Photochem. Photobiol. 83, 1 (2007).
S. Kumar and R. Swaminathan, J. Chem. Sci. 119, 141 (2007).
L. Yu. Brovko, E. Yu. Cherednikova, and A. Yu. Chikishev, Biospectroscopy 5, 378 (1999).
D. M. Togashi and A. G. Ryder, J. Fluoresc. 16, 153 (2006).
B. Mishra, A. Barik, K. Indra Priyadarsint, and H. Mohan, J. Chem. Sci. 117, 641 (2005).
H. G. Mahesha, A. S. Sridevi, N. Srinivasan, and A. G. Appu Rao, FEBS J. 273, 451 (2006).
T.A. Dolenko, V.V. Fadeev, I.V. Gerdova, and R. Reuter, Appl. Opt. 41, 5155 (2002).
J.R. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1999; Mir, Moscow, 1986).
V. V. Fadeev, T. A. Dolenko, E. M. Filippova, and V. V. Chubarov, Opt. Comm. 166, 25 (1999).
A. A. Banishev, D. V. Maslov, and V. V. Fadeev, Instrum. Exp. Tech. 49, 430 (2006).
S. A. Dolenko, T. A. Dolenko, V. V. Fadeev, et al., Opt. Comm. 213, 309 (2002).
V. V. Fadeev, T. A. Dolenko, A. A. Banishev, et al., in Proceedings of SPIE, Conference on Spectroscopy, Dublin, Ireland, 2005, Vol. 5826, pp. 44–55.
A. A. Banishev, E. P. Vrzheshch, D. V. Dmitrienko, et al., Biofizika 52, 792 (2007).
T. Peters, Jr., All About Albumin: Biochemistry, Genetics, and Medical Applications (Academic, San Diego, 1996), p. 121.
M. Eftink, J. L. Zajicek, and C. A. Ghiron, Biochim. Biophys. Acta 491, 473 (1977).
V. Agranovich and M. Galanin, Energy Transfer of Electron Excitation (Nauka, Moskow, 1978), p. 37 [in Russian].
D. V. Maslov, E. E. Ostroumov, and V. V. Fadeev, Quantum Electron. 36, 163 (2006).
A. M. Chekalyuk, V. V. Fadeev, G. Georgyiev, and T. Kalkanjiev, Spec. Lett. 15, 355 (1982).
C. Caratheodory, Calculus of Variations and Partial Differential Equations of First Order (Am. Math. Soc, New York, 1999), p. 95.
S. A. Dolenko, T. A. Dolenko, O. V. Kozyreva, et al., Pattern Recogn. Image Anal. 19, 510 (1999).
A. A. Banishev, E. A. Shirshin, and V. V. Fadeev, Quantum Electron. 38, 76 (2008).
C. N. Pace, F. Vajdos, L. Fee, et al., Protein Sci. 4, 2411 (1995).
S. C. Gill and P. H. von Hippel, Anal. Biochem. 182, 319 (1989).
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Banishev, A.A., Shirshin, E.A. & Fadeev, V.V. Laser fluorimetry of proteins containing one and two tryptophan residues. Laser Phys. 18, 861–867 (2008). https://doi.org/10.1134/S1054660X08070104
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DOI: https://doi.org/10.1134/S1054660X08070104