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Registering the Kinetics of Intermolecular Interactions by Low-Coherence Interferometry for the Development of Biomarker Immunoassays for Cardiovascular Diseases

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Abstract

The quantitative monitoring of kinetic parameters for the interaction of biomolecules is demanded in many fields of fundamental and applied research. In this study, some compact biosensor devices, which are characterized by high energy efficiency and provide ultrasensitive real-time registration of changes in the thickness of biomolecular complexes on widely available disposable sensor chips, have been developed on the basis of low-coherence interferometry. These instruments are electrically and optically safe, can be powered and controlled by a laptop USB port, and have passive drift-suppressing thermal insulation. The potentialities of these instruments are demonstrated on the kinetic characterization of antibodies as an example to select the most efficient ones as immunoassays for the brain natriuretic peptide, which is a marker of cardiovascular diseases. The devices and methods developed provide for efficient selection of optimal biorecognizing agents for in vitro diagnosis, targeted nanopreparation delivery, etc.

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ACKNOWLEDGMENTS

The authors are grateful to I.L. Nikitin for fruitful discussions and help in preparing the manuscript.

Funding

Different aspects of this interdisciplinary study were supported in part by the Russian Science Foundation, grant no. 19-73-10205 for the label-free kinetic characterization of monoclonal antibodies and the optimization of an immunoassay system for the detection of molecular cardiac biomarkers and grant no. 21-12-00407 for the development of methods for surface functionalization and the selection of recognizing molecules and experimental prototypes.

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Authors and Affiliations

  1. Prokhorov Institute of General Physics, Russian Academy of Sciences, 119991, Moscow, Russia

    A. V. Orlov, D. O. Novichikhin, A. V. Pushkarev, Yu. A. Malkerov, S. L. Znoiko, N. V. Guteneva, N. N. Orlova, B. G. Gorshkov & P. I. Nikitin

  2. National Research Nuclear University, Moscow Engineering Physics Institute, 115409, Moscow, Russia

    D. O. Novichikhin, Yu. A. Malkerov & P. I. Nikitin

Authors
  1. A. V. Orlov
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  2. D. O. Novichikhin
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  3. A. V. Pushkarev
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  4. Yu. A. Malkerov
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  5. S. L. Znoiko
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  6. N. V. Guteneva
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  7. N. N. Orlova
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  8. B. G. Gorshkov
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  9. P. I. Nikitin
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Corresponding authors

Correspondence to A. V. Orlov or P. I. Nikitin.

Additional information

Translated by E. Glushachenkova

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Orlov, A.V., Novichikhin, D.O., Pushkarev, A.V. et al. Registering the Kinetics of Intermolecular Interactions by Low-Coherence Interferometry for the Development of Biomarker Immunoassays for Cardiovascular Diseases. Dokl. Phys. 67, 193–196 (2022). https://doi.org/10.1134/S1028335822070035

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  • DOI: https://doi.org/10.1134/S1028335822070035

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