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In Situ Dynamic Light Scattering Complementing Neutron Spin Echo Measurements on Protein Samples

Journal of Surface Investigation: X-ray, Synchrotron and Neutron Techniques volume 14, pages S185–S189 (2020)Cite this article

Abstract

Monitoring the state of the sample on the minute-time scale is crucial in case of sensitive soft matter or biological samples, given that neutron spin echo measurements take up to several days. Moreover, there is no method to interpret the normalized intermediate scattering function obtained by measuring the neutron spin echo if relevant sample properties change during measurement. Dynamic light scattering provides information on the diffusion constant of particles in solution (biological macromolecules, such as proteins, protein aggregates, polymer particles, etc.) with average hydrodynamic radii in a wide range from several nanometers up to several microns. This information can be obtained in a few minutes and it offers a good overview of the current sample state. Details on the novel in situ dynamic light scattering set-up with one fixed scattering angle and first results obtained on a molten globule state of apo-myoglobin are presented.

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Funding

The work was financially partially supported through the BMBF project 05K16PA1.

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Authors and Affiliations

  1. Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany

    L. Balacescu, F. Vögl, S. Staringer, V. Ossovyi, G. Brandl, O. Holderer, S. Pasini & T. E. Schrader

  2. Physikalisches Institut, AG Biophysik, 52074, Rheinisch-Westfälische Technische Hochschule Aachen, Germany

    L. Balacescu & J. Fitter

  3. Forschungszentrum Jülich, Jülich Centre for Neutron Science and Institute for Complex Systems, 52425, Jülich, Germany

    N. Lumma, H. Feilbach & A. Stadler

  4. Forschungszentrum Jülich, Institute for Complex Systems (ICS-5), 52425, Jülich, Germany

    J. Fitter

Authors
  1. L. Balacescu
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  2. F. Vögl
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  3. S. Staringer
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  4. V. Ossovyi
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  5. G. Brandl
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  6. N. Lumma
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  7. H. Feilbach
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  8. O. Holderer
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  9. S. Pasini
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  10. A. Stadler
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  11. J. Fitter
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  12. T. E. Schrader
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Corresponding author

Correspondence to T. E. Schrader.

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Balacescu, L., Vögl, F., Staringer, S. et al. In Situ Dynamic Light Scattering Complementing Neutron Spin Echo Measurements on Protein Samples. J. Surf. Investig. 14 (Suppl 1), S185–S189 (2020). https://doi.org/10.1134/S1027451020070046

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  • DOI: https://doi.org/10.1134/S1027451020070046

Keywords:

  • biopolymers
  • spin echo spectroscopy
  • in situ dynamic light scattering
  • aggregation