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AFM study of the extracellular connective tissue matrix in patients with pelvic organ prolapse

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Abstract

The method of atomic force microscopy (AFM) is used for the first time for morphological investigation of pathological changes in the extracellular matrix of skin connective tissue upon the prolapse of pelvic organs (common disorder among women). Skin samples of patients with clinically proven pelvic-organ prolapse and of patients that do not have any connective tissue related disease (control group) are investigated via AFM. The AFM study reveals that the extracellular matrix of the skin connective tissues from patients with pelvic-organ prolapse diverges from the normal in various organization levels including both micro- and nanotexture (packing of collagen fibers and fibrils, respectively). The results of AFM study of the normal and pathologically changed skin connective tissues are in good agreement with the data of clinical morphological analysis, which indicates the potential of AFM as an independent diagnostic tool.

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Authors and Affiliations

  1. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, 119991, Russia

    S. L. Kotova, V. A. Timofeeva & A. B. Solovieva

  2. First Moscow State Medical University, Moscow, 119991, Russia

    A. B. Shekhter & A. E. Guller

  3. Institute of Laser and Information Technology Problems, Russian Academy of Sciences, Moscow, 119971, Russia

    P. S. Timashev, V. Ya. Panchenko & V. N. Bagratashvili

  4. Karpov Institute of Physical Chemistry, Moscow, 105064, Russia

    P. S. Timashev

  5. State Scientific Center of Coloproctology, Moscow, 123423, Russia

    A. A. Mudrov

Authors
  1. S. L. Kotova
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  2. A. B. Shekhter
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  3. P. S. Timashev
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  4. A. E. Guller
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  5. A. A. Mudrov
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  6. V. A. Timofeeva
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  7. V. Ya. Panchenko
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  8. V. N. Bagratashvili
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  9. A. B. Solovieva
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Corresponding author

Correspondence to S. L. Kotova.

Additional information

Original Russian Text © S.L. Kotova, A.B. Shekhter, P.S. Timashev, A.E. Guller, A.A. Mudrov, V.A. Timofeeva, V.Ya. Panchenko, V.N. Bagratashvili, A.B. Solovieva, 2014, published in Poverkhnost’. Rentgenovskie, Sinkhrotronnye i Neitronnye Issledovaniya, 2014, No. 8, pp. 24–31.

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Kotova, S.L., Shekhter, A.B., Timashev, P.S. et al. AFM study of the extracellular connective tissue matrix in patients with pelvic organ prolapse. J. Surf. Investig. 8, 754–760 (2014). https://doi.org/10.1134/S1027451014040260

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  • DOI: https://doi.org/10.1134/S1027451014040260

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