Atmospheric and Oceanic Optics

, Volume 31, Issue 4, pp 390–396 | Cite as

Spectroscopic Study of Green Sulfur Bacteria in Stratified Water Bodies of the Kandalaksha Gulf of the White Sea

  • A. A. ZhiltsovaEmail author
  • A. V. Kharcheva
  • E. D. Krasnova
  • O. N. Lunina
  • D. A. Voronov
  • A. S. Savvichev
  • O. M. Gorshkova
  • S. V. Patsaeva
Optical Instrumentation


Optical characteristics of water in stratified lakes of the White Sea are of particular interest in connection with the observation of thin colored layers resulting from massive development of anoxygenic phototrophic bacteria around the chemocline. While optical properties of chlorophyll are widely used in remote sensing, spectral characteristics of bacteriochlorophylls (BChl) for natural microbial communities have been little studied. In this work, spectral study of green sulfur bacteria of four water bodies of the Kandalaksha Gulf of the White Sea was carried out. Absorption and fluorescence spectra were measured for water samples taken in March 2017 from different depths and compared with spectra of monocultures isolated from the same water bodies earlier. It has been shown that the BChl fluorescence in the living cells of green sulfur bacteria has two overlapping emission bands: in the region of 740–770 nm (BChl d and e) and at 815 nm (BChl a). The wavelength of the maximum of the first band depends on the ratio of the concentrations of green- and brown-colored forms of bacteria containing different types of BChl. A new method for separating the contributions of two types of bacteria is proposed, based on the deconvolution of the BChl fluorescence spectrum into three bands whose parameters are determined from the spectra of monocultures. The BChl content at various water depths has been estimated and the percentage ratio of different types of phototrophic bacteria has been determined.


anoxygenic phototrophic bacteria green sulfur bacteria fluorescence absorption bacteriochlorophyll the White Sea 


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  1. 1.
    G. Oostergetel, H. van Amerongen, and E. Boekema, “The chlorosome: A prototype for efficient light harvesting in photosynthesis,” Photosynth. Res. 104 (2-3), 245–255 (2010).CrossRefGoogle Scholar
  2. 2.
    Ecology of Meromictic Lakes, Ed. by R. Gulati, E. Zadereev, and A. Degermendzhi (Springer, 2017).Google Scholar
  3. 3.
    E. D. Krasnova and A. N. Pantyulin, “Sour-and-Sweet lakes full of the wonders,” Priroda, No. 2, 39–48 (2013).Google Scholar
  4. 4.
    E. D. Krasnova, D. A. Voronov, N. A. Demidenko, N. M. Kokryatskaya, A. N. Pantyulin, T. A. Rogatykh, T. E. Samsonov, and N. L. Frolova, “Study of separating water basins on the White Sea coast. Complex study of the Bab’e More—a semi-isolated White Sea lagoon: Geology, hydrology, and biota, variations against the coastal transgression, in Proc. of the White Sea Biological Station of Moscow State University (KMK, Moscow, 2016), vol. 12, pp. 211–241 [in Russian].Google Scholar
  5. 5.
    O. N. Lunina, A. S. Savvichev, B. B. Kuznetsov, N. V. Pimenov, and V. M. Gorlenko, “Phototrophic anoxic bacteria of the stratified Kislo-Sladkoe lake (Kandalaksha Gulf of the White Sea),” Mikrobiologiya 83 (1), 90–108 (2014).Google Scholar
  6. 6.
    E. D. Krasnova, D. A. Voronov, N. Frolova, A. Pantyulin, and T. Samsonov, “Salt lakes separated from the White Sea,” EARSeL eProc. 14, 8–22 (2015).Google Scholar
  7. 7.
    The White Sea System. Water depth and Interacting Atmosphere, Cryosphere, River Inflow, and Biosphere, Ed. by A. P. Lisitsyn (Nauchnyi mir, Moscow, 2012), vol. 2, pp. 433–579 [in Russian].Google Scholar
  8. 8.
    F. A. Romanenko and O. S. Shilova, “The postglacial uplift of the Karelian coast of the White Sea according to radiocarbon and diatom analyses of lacustrine-boggy deposits of Kindo Peninsula,” Dokl. Earth Sci. 442 (2), 242–246 (2012).ADSCrossRefGoogle Scholar
  9. 9.
    A. V. Kharcheva, A. V. Meschankin, I. I. Lyalin, E.D. Krasnova, D. A. Voronov, and S. V. Patsaeva, “The study of coastal meromictic water basins in the Kandalaksha Gulf of the White Sea by spectral and physicochemical methods,” Proc. SPIE 9031, 90310 (2014).ADSGoogle Scholar
  10. 10.
    A. V. Kharcheva, E. D. Krasnova, D. A. Voronov, and S. V. Patsaeva, “Spectroscopic study of the microbial community in chemocline zones of relic meromictic lakes separating from the White Sea,” Proc. SPIE 9448, 94480 (2015).ADSGoogle Scholar
  11. 11.
    E. Krasnova, A. Kharcheva, I. Milyutina, D. Voronov, and S. Patsaeva, “Study of microbial communities in redox zone of meromictic lakes isolated from the White Sea using spectral and molecular methods,” J. Mar. Biol. Assoc. U. K. 95 (8), 1579–1590 (2015).CrossRefGoogle Scholar
  12. 12.
    A. V. Kharcheva, E. D. Krasnova, V. M. Gorlenko, O. N. Lunina, A. S. Savvichev, D. A. Voronov, A. A. Zhiltsova, and S. V. Patsaeva, “Depth profiles of spectral and hydrological characteristics of water and their relation to abundances of green sulfur bacteria in the stratified lakes of the White Sea,” Proc. SPIE 9917, 99170 (2016).ADSGoogle Scholar
  13. 13.
    V. I. Mishanin, B. V. Trubitsin, S. V. Patsaeva, V. V. Ptushenko, A. E. Solovchenko, and A. N. Tikhonov, “Acclimation of shade-tolerant and light-resistant tradescantia species to growth light: Chlorophyll a fluorescence, electron transport, and xanthophyll content,” Photosynth. Res. 133 (1-3), 87–102 (2017).CrossRefGoogle Scholar
  14. 14.
    V. A. Terekhova and M. M. Gladkova, “Engineered nanomaterials in soil: Problems in assessing their effect on living organisms,” Eurasian Soil Sci. 46 (12), 1203–1210 (2013).ADSCrossRefGoogle Scholar
  15. 15.
    V. Pelevin, A. Zlinszky, E. Khimchenko, and V. Toth, “Ground truth data on chlorophyll-a, chromophoric dissolved organic matter and suspended sediment concentrations in the upper water layer as obtained by LIF lidar at high spatial resolution,” Int. J. Remote Sens. 38 (7), 1967–1982 (2017).ADSCrossRefGoogle Scholar
  16. 16.
    I. A. Sutorikhin, V. I. Bukatyi, and O. B. Akulova, “Seasonal changes of water spectral transparency and concentration of chlorophyll a in different-type lakes,” Opt. Atmos. Okeana 27 (9), 801–806 (2014).Google Scholar
  17. 17.
    G. S. Orf and R. E. Blankenship, “Chlorosome antenna complexes from green photosynthetic bacteria,” Photosynth. Res. 16, 315–331 (2013).CrossRefGoogle Scholar
  18. 18.
    J. M. Olson, “Chlorophyll organization and function in green photosynthetic bacteria,” Photochem. Photobiol. 67 (1), 61–75 (1998).CrossRefGoogle Scholar
  19. 19.
    U. Feiler and G. Hauska, “The reaction center from Green Sulfur Bacteria,” in Anoxygenic Photosynthetic Bacteria, Ed. by R.E. Blankenship, M.T. Madigan, and C.E. Bauer (Springer, 1995), pp. 665–685.Google Scholar
  20. 20.
    A. Yakovlev, V. Novoderezhkin, A. Taisova, V. Shuvalov, and Z. Fetisova, “Orientation of B798 BChl a Q y transition dipoles in Chloroflexus aurantiacus chlorosomes: Polarized transient absorption spectroscopy studies,” Photosynth. Res. 125 (1-2), 31–42 (2015).CrossRefGoogle Scholar
  21. 21.
    J. Overmann and M. M. Tilzer, “Control of primary productivity and the significance of photosynthetic bacteria in a meromictic kettle lake Mittlerer Buchensee, West-Germany,” Aquat. Sci. 51, 261–278 (1989).CrossRefGoogle Scholar
  22. 22.
    E. A. Nusch, “Comparison of different methods for chlorophyll and phaeopigment determination,” Arch. Hydrobiol. Beith. Ergebn. Limnol. 14, 14–36 (1980).Google Scholar
  23. 23.
    “Photosynthetic microorganisms,” in Proc. of Vinogradskii Institute of Micorbiology, Ed. by V. F. Gal’chenko (MAKS Press, Moscow, 2010), is. 15, pp. 133–175 [in Russian].Google Scholar
  24. 24.
    A. V. Kharcheva, A. A. Zhiltsova, O. N. Lunina, A. S. Savvichev, and S. V. Patsaeva, “Quantification of two forms of green sulfur bacteria in their natural habitat using bacteriochlorophyll fluorescence spectra,” Proc. SPIE 9917, 99170 (2016).ADSGoogle Scholar
  25. 25.
    A. V. Kharcheva, A. A. Zhiltsova, O. N. Lunina, E. D. Krasnova, D. A. Voronov, A. S. Savvichev, and S. V. Patsaeva, “Fluorescence of bacteriochlorophylls of green sulfur bacteria in the anaerobic zone of two natural waterbodies,” Bull. Moscow State Univ. Phys., Astron. (in print).Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • A. A. Zhiltsova
    • 1
    Email author
  • A. V. Kharcheva
    • 1
  • E. D. Krasnova
    • 2
  • O. N. Lunina
    • 5
  • D. A. Voronov
    • 3
    • 6
  • A. S. Savvichev
    • 5
  • O. M. Gorshkova
    • 4
  • S. V. Patsaeva
    • 1
  1. 1.Department of PhysicsMoscow State UniversityMoscowRussia
  2. 2.Department of BiologyMoscow State UniversityMoscowRussia
  3. 3.Belozersky Institute of Physico-Chemical BiologyMoscow State UniversityMoscowRussia
  4. 4.Department of GeographyMoscow State UniversityMoscowRussia
  5. 5.Winogradsky Institute of Microbiology, Federal Research Centre Fundamentals of BiotechnologyRussian Academy of SciencesMoscowRussia
  6. 6.Kharkevich Institute for Information Transmission ProblemsRussian Academy of SciencesMoscowRussia

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