Abstract
In this work, an explicit analytical estimate of the absorption cross section of homogenous weakly refracting nonspherical particles and their suspensions is considered and justified. By an example of Spirulina platensis, a way of estimating the spectrum of pigment absorption coefficients in the region of photosynthetically active radiation has been implemented without the use of pigment extracts. The effect of the cell size and shape distribution on absorption spectra is studied. The intracellular concentration of chlorophyll a, phycoerythrin, and phycocyanin is estimated.
Similar content being viewed by others
References
A. Morel, “Light and marine photosynthesis: A spectral model with geochemical and climatological implications,” Prog. Oceanogr. 26, 263–306 (1991).
T. Platt and S. Sathyendranath, “Oceanic primary production: Estimation by remote sensing at local and regional scales,” Science 241, 1613–1620 (1988).
A. Bricaud, A. Morel, M. Babin, K. Allali, and H. Claustre, “Variations of light absorption by suspended particles with chlorophyll a concentration in oceanic (case 1) waters: Analysis and implications for bio-optical models,” J. Geophys. Res. 103, 31033–31044 (1998).
S. Sathyendranath, G. Cota, V. Stuart, H. Maass, and T. Platt, “Remote sensing of phytoplankton pigments: A comparison of empirical and theoretical approaches,” Int. J. Remote Sens. 22, 249–273 (2001).
D. Ficek, S. Kaczmarek, J. Ston-Egiert, B. Wozniak, R. Majchrowski, and J. Dera, “Spectra of light absorption by phytoplankton pigments in the Baltic: Conclusions to be drawn from a Gaussian analysis of empirical data,” Oceanologia 46, 533–555 (2004).
J. T. O. Kirk, Light and Photosynthesis in Aquatic Ecosystems (University Press, Cambridge, 2011), 3rd ed.
L. M. N. Duysens, “The flattening effect of the absorption spectra of suspensions as compared to that of solutions,” Biochim. Biophys. Acta 19, 1–12 (1956).
N. Hoepffner and S. Sathyendranath, “Effect of pigment composition on absorption properties of phytoplankton,” Mar. Ecol.: Prog. Ser. 73, 11–23 (1991).
S. G. H. Simis and H. M. Kauko, “In vivo mass-specific absorption spectra of phycobilipigments through selective bleaching,” Limnol. Oceanogr.: Methods 10, 214–226 (2012).
L. E. Paramonov, E. B. Khromechek, V. V. Abdulkin, and V. A. Shmidt, “Solving the inverse problems on equivalent classes,” Atmos. Ocean. Opt. 17 (5-6), 453–457 (2004).
H. C. van de Hulst, Light Scattering by Small Particles (Wiley and Sons, New York; Chapman and Hall, London, 1957).
K.S. Shifrin and G. Tonna, “Simple equation for the absorption coefficient of weakly-refracting particles,” Opt. Spektrosk. 72, 487–490 (1992).
L. E. Paramonov, “Optical equivalence of isotropic ensembles of ellipsoidal particles in the Rayleigh–Gans–Debye and anomalous diffraction approximations and its consequences,” Opt. Spectrosc. 112 (5), 787–795 (2012).
P. C. Waterman, “Symmetry, unitarity and geometry in electromagnetic scattering,” Phys. Rev., D 3, 825–839 (1970).
L. E. Paramonov, “T-matrix approach and the angular momentum theory in light-scattering problems by ensembles of arbitrarily shaped particles,” J. Opt. Soc. Am., A 12, 2698–2707 (1995).
J. T. O. Kirk, “A theoretical analysis of the contribution of algal cells to the attenuation of light within natural waters. III. Cylindrical and spheroidal cells,” New Phytol. 77 (2), 341–358 (1976).
F. I. Kuzminov, E. A. Shirshin, M. Yu. Gorbunov, and V. V. Fadeev, “New optical approaches in studying photophysiological parameters of cyanobacteria in situ,” Fundam. Prikl. Gidrofiz. 8 (1), 41–47 (2015).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © L.E. Paramonov, 2018, published in Optika Atmosfery i Okeana.
Rights and permissions
About this article
Cite this article
Paramonov, L.E. Absorption Coefficient Spectrum and Intracellular Pigment Concentration by an Example of Spirulina platensis. Atmos Ocean Opt 31, 263–268 (2018). https://doi.org/10.1134/S1024856018030107
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1024856018030107