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Atmospheric and Oceanic Optics

, Volume 27, Issue 6, pp 479–486 | Cite as

Insecticidal, antimicrobial, and antiviral activity of Bacillus thuringiensis ssp. kurstaki strains isolated from atmospheric aerosols in the South of Western Siberia

  • I. S. AndreevaEmail author
  • A. S. Safatov
  • O. S. Mokrushina
  • G. A. Buryak
  • L. I. Puchkova
  • N. A. Mazurkova
  • L. I. Burtseva
  • G. V. Kalmykova
Optics of Clusters, Aerosols, and Hydrosoles
  • 58 Downloads

Abstract

The data on insecticidal, antimicrobial, and antiviral activity of Bacillus thuringiensis (Bt) strains isolated in the microbiological study of height and ground atmospheric aerosol samples taken in the South of Western Siberia are presented. Among 36 isolated bacteria determined as related to the Bt species, 15 strains were relegated to the Bt ssp. kurstaki subspecies by results of intraspecific testing and studied for the presence of antagonistic attributes. In spite of long wandering in extreme conditions in the composition of atmospheric aerosols, including higher atmospheric layer (up to 7 km), the isolated Bt ssp. kurstaki strains keep high (inherent to this subspecies) entomopathogenic activity, possessed the ability to inhibit the multiplication of such pathogenic microorganisms as Staphylococcus aureus, Salmonella thyphimurium, Shigella sonnei, Candida albicans, and others, and manifested the ability to effectively neutralize the multiplication of the A/chicken/Kurgan/05/2005 (A/H5N1) avian influenza virus and A/Aichi/2/68 (A/H3N2) human virus. Properties of Bt strains isolated from atmospheric aerosols not only verified their well-known resistance to unfavorable factors of the environment but also exhibited the possibility of using them for developing viricides.

Keywords

atmospheric aerosols Bacillus thuringiensis insecticidal antimicrobial and antiviral activity 

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References

  1. 1.
    S. D. Pillai, K. W. Widmer, S. E. Dowd, and S. C. Ricke, “Occurrence of airbone bacteria and pathogen indicators during land application of sewage sludge,” Appl. Environ. Microbiol. 62(1), 296–299 (1996).Google Scholar
  2. 2.
    C. I. Davidson, R. F. Phalen, and P. A. Solomon, “Airborne particulate matter and human health: A review,” Aerosol Sci. Technol. 39(8), 737–749 (2005).CrossRefGoogle Scholar
  3. 3.
    C. M. O’ Gorman and H. T. Fuller, “Prevalence of culturable airborne spores of selected allergenic and pathogenic fungi in outdoor air,” Atmos. Environ. 42(18), 4355–4368 (2008).ADSCrossRefGoogle Scholar
  4. 4.
    Patogens of Insects: Structural and Functional Aspects, Ed. by V. V. Glupov (Kruglyi god, Moscow, 2001) [in Russian].Google Scholar
  5. 5.
    P. Boonserm, P. Davis, D. J. Ellar, and J. Li, “Crystal structure of the mosquito-larvicidal toxin Cry4Ba and its biological implications,” J. Mol. Biol. 348(2), 363–382 (2005).CrossRefGoogle Scholar
  6. 6.
    C. Hofmann, P. Luthy, R. Hutter, and V. Pliska, “Binding of the delta-endotoxin from Bacillus thuringiensis to brush-border membrane vesicles of the cabbage butterfly (Pieris brassicae),” Eur. J. Biochem. 173(1), 85–91 (1988).CrossRefGoogle Scholar
  7. 7.
    A. Bravo, S. Gill, and M. Soberon, “Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control,” Toxicon 49(4), 423–435 (2007).CrossRefGoogle Scholar
  8. 8.
    L. K. Kamenek, E. G. Klimentova, and A. A. Tyul’pineva, “Action of delta-endotoxin Bacillus thuringiensis subsp. kurstaki on some aerobic phytopathogenic bacteria,” (Uch. zap. UlGU, Ul’yanovsk, 2000), Part 1(2) [in Russian].Google Scholar
  9. 9.
    T. G. Yudina, I. A. Zalunin, L. A. Kirsanova, Dan’yan Go, N. V. Bulushova, and A. I. Netrusov, “Comparison of antimicrobic action Cry and Cyt of Bacillus thuringiensis proteins and their fragments,” Byull. MOIP, Biolog. 114 (2009).Google Scholar
  10. 10.
    V. P. Vasil’ev, Crop Pests and Insects. Vol. 3. Techniques and Means for Fighting with Insects, Systems of Measures on Plant protection, Ed. By V. P. Vasil’ev and V. P. Omeluta (Urozhai, Kiev, 1989) [in Russian].Google Scholar
  11. 11.
    A. Bravo, S. Likitvivatanavong, S. S. Gill, and M. Soberon, “Bacillus thuringiensis: A story of a successful bioinsecticide,” Insect Biochem. Mol. Biol. 41(7), 423–431 (2011). www.bcn.ca/stop/part4.html.CrossRefGoogle Scholar
  12. 12.
    P. H. Damgaard, H. D. Larsen, B. M. Hansen, J. Bresciani, and K. Jorgensen, “Enterotoxin-producing strains of Bacillus thuringiensis isolated from food,” Lett. Appl. Microbiol. 23(3), 146–150 (2008).CrossRefGoogle Scholar
  13. 13.
    I. S. Andreeva, B. D. Belan, A. I. Borodulin, G. A. Buryak, Yu. V. Marchenko, S. E. Ol’kin, M. V. Panchenko, V. A. Petrishchenko, O. V. P’yankov, I. K. Reznikova, A. S. Safatov, A. N. Sergeev, and E. V. Stepanova, “Variability of biogenic component of atmospheric aerosol over forested areas of Western Siberia,” Atmos. Ocean. Opt. 13(6–7), 592–596 (2000).Google Scholar
  14. 14.
    I. S. Andreeva, A. I. Borodulin, G. A. Buryak, V. A. Zhukov, S. V. Zykov, Yu. V. Marchenko, V. V. Marchenko, S. E. Ol’kin, V. A. Petrishchenko, O. V. P’yankov, I. K. Reznikova, V. E. Repin, A. S. Safatov, A. N. Sergeev, V. F. Raputa, V. V. Penenko, E. A. Tsvetova, M. Yu. Arshinov, B. D. Belan, M. V. Panchenko, A. N. Ankilov, A. M. Baklanov, A. L. Vlasenko, K. P. Kutsenogii, V. I. Makarov, and T. V. Churkina, “Biogenic component of atmospheric aerosol in the south of West Siberia,” Chem. Sustain. Develop., No. 5, 523–527 (2002).Google Scholar
  15. 15.
    A. Safatov, G. Buryak, I. Andreeva, S. Olkin, I. Reznikova, A. Sergeev, B. Belan, M. Panchenko, D. Simonenkov, and G. Tolmachev, “Altitude profiles of biogenic components of atmospheric aerosols in southwestern Siberia,” Chem. Eng. Transact. 16, 225–232 (2008).Google Scholar
  16. 16.
    A. N. Sergeev, A. S. Safatov, A. P. Agafonov, I. S. Andreeva, M. Yu. Arshinov, B. D. Belan, G. A. Buryak, V. M. Generalov, Yu. R. Zakharova, N. A. Lapteva, S. E. Ol’kin, M. V. Panchenko, V. V. Parfenova, I.K. Reznikova, D. V. Simonenkov, T. V. Teplyakova, and V. A. Ternovoi, “The comparison of the presence of chemical and biological markers in the surface microlayer of water areas of health resort zones at Lake Baikal and aerosol of this region,” Opt. Atmosf. Okeana 22(6), 585–594 (2009).Google Scholar
  17. 17.
    A. S. Safatov, G. A. Buryak, S. E. Ol’kin, I. K. Reznikova, Yu. V. Marchenko, B. M. Desyatkov, N. A. Lapteva, I. S. Andreeva, A. S. Kozlov, S. B. Malyshkin, I. A. Sutorikhin, V. I. Bukatyi, S. A. Litvinenko, B. S. Smolyakov, and M. P. Shinkorenko, “Results of the complex ecological study of water and near-shore air of bitter-salty lakes of Altai region in summer of 2011,” Opt. Atmosf. Okeana 25(6), 550–558 (2012).Google Scholar
  18. 18.
    Methods for General and Molecular Microbiology, Ed. by F. Gerhard, R. G. E. Murray, W. A. Wood, and N. Krieg (American Society for Microbiology, Washington, DC., 1981), vol. 3.Google Scholar
  19. 19.
    Bergey’s Manual of Systematic Bacteriology, Ed. by J. G. Holt (Williams and Wilkins, Baltimore, London, 1986), vol. 1–2.Google Scholar
  20. 20.
    P. Fredericq, Actions Antibiotiques Reciproques chez les Enterobacteriaceae (Duculot, 1948).Google Scholar
  21. 21.
    I. S. Andreeva, N. A. Mazurkova, O. S. Mokrushina, L. I. Puchkova, and A. I. Zakabunin, “Bacillus thuringiensis strains secreting metabolites inhibit the replication of human influenza virus A/Aichi/2/68 (H3N2) and avian influenza virus A/chicken/Kurgan/05/2005,” Mezhdunar. Nauch.-Issled. Zh., No. 7-1, 70–71 (2013).Google Scholar
  22. 22.
    V. A. Sakanyan, G. N. Selivanova, N. O. Bukanov, M. A. Krupenko, and S. I. Alikhanyan, “Propagation, homology, and cloning of cryptic plasmids Bacillus thuringiensis,” Genetika 18(2), 181–190 (1982).Google Scholar
  23. 23.
    F. P. Galushka and P. P. Azizbekyan, “Study of plasmids of different strains of Bacillus thuringiensis,” Dokl. Akad. Nauk SSSR 236(5), 1233–1235 (1977).Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • I. S. Andreeva
    • 1
    Email author
  • A. S. Safatov
    • 1
  • O. S. Mokrushina
    • 1
  • G. A. Buryak
    • 1
  • L. I. Puchkova
    • 1
  • N. A. Mazurkova
    • 1
  • L. I. Burtseva
    • 2
  • G. V. Kalmykova
    • 3
  1. 1.State Research Center of Virology and Biotechnology “Vector”Koltsovo, Novosibirsk oblastRussia
  2. 2.Institute of Systematics and Ecology of Animals, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  3. 3.Siberian Scientific Research Institute of Agricultural ProcessingRussian Agricultural AcademyKrasnoobsk, Novosibirsk oblastRussia

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