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A novel electrochemical biosensor for detection of cholesterol

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Abstract

We report on a highly sensitive electrochemical biosensor for determination of cholesterol. The biosensor was fabricated by co-immobilizing bi-enzymes, cholesterol oxidase (ChOx), and horseradish peroxidase (HRP). Voltammetric technique such as cyclic voltammetry and impedance experiment were used to study the characterization of modified electrode step by step. The developed sensor is cheap, disposable, portable and exhibits higher sensitivity. The biosensor expressed a wide linear range up to 300 mg dL–1 in a physiological condition (pH 7.0), with a correlation coefficient of 0.9969. A sensitivity of 13.28 μA mg–1 dL cm−2 which makes it very promising for the clinical determination of cholesterol.

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering and College of Science, Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, China

    Lin Xu & Cheng Yao

  2. Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), National Jiangsu Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, China

    Mengdan Zhang, Xin Yang, Wei Huang & Qiong Wu

  3. Key Laboratory for Organic Electronics & Information Displays (KLOEID), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Yadong, Xincheng District, Nanjing, 210046, P. R. China

    Yiting Hou & Greath Jenkins

  4. Nan Jing College of Chemical Technology, 625 GeGuan Road, Nanjing, 210048, China

    Lin Xu

Authors
  1. Lin Xu
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  2. Yiting Hou
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  3. Mengdan Zhang
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  4. Xin Yang
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  5. Greath Jenkins
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  6. Wei Huang
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  7. Cheng Yao
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  8. Qiong Wu
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Corresponding authors

Correspondence to Cheng Yao or Qiong Wu.

Additional information

Published in Russian in Elektrokhimiya, 2016, Vol. 52, No. 3, pp. 278–284.

The article is published in the original.

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Xu, L., Hou, Y., Zhang, M. et al. A novel electrochemical biosensor for detection of cholesterol. Russ J Electrochem 52, 239–244 (2016). https://doi.org/10.1134/S1023193516030101

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  • DOI: https://doi.org/10.1134/S1023193516030101

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