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In vitro study of the interaction of cysteine with a thiazide diuretic (hydrochlorothiazide) at different ph by voltammetric and spectroscopic techniques

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Abstract

The interaction of cysteine (RSH) with a thiazide diuretic, hydrochlorothiazide (HCTZ) was characterized by UV-Vis absorption spectroscopy and square-wave voltammetry in Britton-Robinson (B-R) buffer solution (with pH 5, 7 and 9). On the square-wave voltamogram of cysteine, the reduction peak current of mercurous cysteine thiolate (Hg2(RS)2) decreased and its peak potential shifted to more positive values with the addition of HCTZ. This results showed that the RSH interacted with HCTZ. The stoichiometry of HCTZ-RSH molecular complex was determined by voltammetric data with the result of 1: 1. By using linear regression analysis of the voltammetric data at pH 5, 7 and 9, the apparent formation constants of HCTZ-RSH complex were calculated to be 9.54 × 103, 2.80 × 104 and 2.55 × 104 M−1, respectively. At the same time, this interaction was also supported by UV-Vis spectroscopic measurements. According to the voltammetric and spectroscopic results, it was suggested that the interaction mode between RSH and HCTZ molecules might be a combination of hydrophobic interactions and hydrogen bonds.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Arts and Sciences, Ondokuz Mayis University, 55139, Kurupelit-Samsun, Turkey

    Ender Biçer & Neslihan Özdemir

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  1. Ender Biçer
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  2. Neslihan Özdemir
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Correspondence to Ender Biçer.

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Published in Russian in Elektrokhimiya, 2013, Vol. 49, No. 10, pp. 1060–1066.

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Biçer, E., Özdemir, N. In vitro study of the interaction of cysteine with a thiazide diuretic (hydrochlorothiazide) at different ph by voltammetric and spectroscopic techniques. Russ J Electrochem 49, 948–954 (2013). https://doi.org/10.1134/S1023193513100042

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  • DOI: https://doi.org/10.1134/S1023193513100042

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