Abstract
Bioelectrocatalytic oxidation of ethanol by whole cells and membrane fraction of Gluconobacter oxydans bacteria is studied on modified graphite-paste electrodes in mediator biosensors. Ferrocene derivatives are used as electron transport mediators for effective coupling of enzymatic and electrochemical processes on graphite electrodes. Electrochemical kinetics of the processes are studied; the obtained data are interpreted in the terms of the mechanism of two-substrate enzymatic reaction. It is shown that mediators of ferrocene series are promising compounds for development of mediator biosensors based both on whole cells of Gluconobacter oxydans bacteria and on membrane fractions of these bacteria. Bioelectrocatalytic processes of ethanol oxidation on graphite paste electrodes occur more efficiently when the bacterial membrane fraction is used as a biocatalyst and ferrocenemonocarboxylic acid is used as a mediator.
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Chaubey, A. and Malholtra, B.D., Biosens. Bioelectron., 2002, vol. 17, p. 441.
Zayats, M., Willner, B., and Willner, I., Electroanalysis, 2008, vol. 20, no. 6, p. 583.
Ding, L., Du, D., Zhang, X., and Ju, H., Curr. Med. Chem, 2008, vol. 15, no. 30, p. 3160.
Tkac, J., Svitel, J., Vostiar, I., Navratil, M., and Gemeiner, P., Bioelectrochemistry, 2009, vol. 76, nos. 1–2, p. 53.
Zhao, F., Slade, R., and Varcoe, J., Chem. Soc. Rev., 2009, vol. 38, no. 7, p. 1926.
Ikeda, T., Kurosaki, T., Takayama, K., and Kano, K., Anal. Chem., 1996, vol. 68, p. 192.
Tkac, J., Gemeiner, P., Svitel, J., Benikovsky, T., Sturdik, E., Vala, V., Petrus, L., and Hrabarova, E., Anal. Chim. Acta, 2000, no. 420, p. 1.
Tkac, J., Vostiar, I., Gorton, Lo., Gemeiner, P., and Sturdik, E., Biosensors Bioelectronics, 2003, no. 18, p. 1125.
Chigrinova, E.Yu., Babkina, E.E., Ponamoreva, O.N., Alferov, V.A., and Reshetilov, A.N., Sens. Sist., 2007, vol. 21, no. 3, p. 263.
Reshetilov, A., Alferov, S., Tomashevskaya, L., and Ponamoreva, O., Electroanalysis, 2006, vol. 18, nos. 19–20, p. 2030.
Alferov, S.V., Tomashevskaya, L.G., Ponamoreva, O.N., Bogdanovskaya, V.A., and Reshetilov, A.N., Elektrokhimiya, 2006, vol. 42, no. 4, p. 456 [Russ. J. Electrochem., 2006, vol. 42, no. 4, p. 403].
Babkina, E., Chigrinova, E., Ponamoreva, O., Alferov, V., and Reshetilov, A., Electroanalysis, 2006, vol. 18, nos. 19–20, p. 2023.
Babkina, E.E., Ponamoreva, O.N., Alferov, V.A., Reshetilov, A.N., and Bogdanovskaya, V.A., Sens. Sist., 2006, vol. 20, no. 4, p. 329.
Muynsk, C., Pereira, C., Naessens, M., Parmentier, S., Soetaert, W., and Vandamme, E., Critical Reviews in Biotechnology, 2007, no. 27, p. 147.
Lapenaite, I., Kurtinaitiene, B., Razumiene, J., Laurinavicius, V., Marcinkeviciene, L., Bachmatova, I., Meskys, R., and Ramanavicius, A., Anal. Chim. Acta, 2005, vol. 549, p. 2030.
Perevalova, E.G., Reshetova, M.D., and Granberg, K.I., Metody elementoorganicheskoi khimii. Ferrotsen (Methods of Organoelement Chemistry. Ferrocene), Moscow: Nauka, 1983.
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Original Russian Text © O.N. Ponamoreva, E.Yu. Indzhgiya, V.A. Alferov, A.N. Reshetilov, 2010, published in Elektrokhimiya, 2010, Vol. 46, No. 12, pp. 1503–1508.
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Ponamoreva, O.N., Indzhgiya, E.Y., Alferov, V.A. et al. Efficiency of bioelectrocatalytic oxidation of ethanol by whole cells and membrane fractions of Gluconobacter Oxydans bacteria in the presence of mediators of ferrocene series. Russ J Electrochem 46, 1408–1413 (2010). https://doi.org/10.1134/S1023193510120116
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DOI: https://doi.org/10.1134/S1023193510120116