Skip to main content
SpringerLink
Log in

Fabrication of multilayer-film-modified gold electrode composed of myoglobin, chitosan, and polyelectrolyte-wrapped multi-wall carbon nanotubes by layer-by-layer assembled technique and electrochemical catalysis for hydrogen peroxide and trichloroacetic acid

  • Published:
Russian Journal of Electrochemistry Aims and scope Submit manuscript

Abstract

Electroactive multilayer film of myoglobin (Mb)-, chitosan (CS)-, and poly(dimethyldiallylammonium chloride) (PDDA)-wrapped multi-wall carbon nanotubes (MWNTs) is fabricated on a gold electrode via layer-by-layer (LBL) technique. The assembled multilayer films is characterized by scanning electron microscopy (SEM), UV-vis spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). UV-vis spectroscopy showed that Mb in the films retained its near-native structure. The stable multilayerfilm-modified gold electrodes showed good electroactivity in protein-free buffer solution, which is originated from protein heme Fe(III)/Fe(II) redox couple. The modified electrode exhibited good electrocatalytic property toward reduction of H2O2 and trichloroacetic acid, indicating the potential application as amperometric biosensor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Decher, G., Science, 1997, vol. 277, p. 1232.

    Article  CAS  Google Scholar 

  2. Wang, L.W. and Hu, N.F., Bioelectrochemistry, 2001, vol. 53, p. 205.

    Article  CAS  Google Scholar 

  3. Liu, H.Y., Rusling, J.F., and Hu, N.F., Langmuir, 2004, vol. 20, p. 10 700.

    CAS  Google Scholar 

  4. Cao, D.F. and Hu, N.F., Biophys. Chem., 2006, vol. 121, p. 209.

    Article  CAS  Google Scholar 

  5. Zhang, H., Lu, H.Y., and Hu, N.F., J. Phys. Chem. B, 2006, vol. 110, p. 2171.

    Article  CAS  Google Scholar 

  6. Lvov, Y.M., Lu, Z.Q., Schenkman, J.B., Zu, X.L., and Rusling, J.F., J. Am. Chem. Soc., 1998, vol. 120, p. 4073.

    Article  CAS  Google Scholar 

  7. Zileinski, B.A. and Aebischer, P., Biomaterials, 1994, vol. 15, p. 1049.

    Article  Google Scholar 

  8. Amiji, M.M., Carbohydr. Polym., 1997, vol. 32, p. 193.

    Article  CAS  Google Scholar 

  9. Yang, M.C. and Lin, W.C., J. Polymer Research, 2002, vol. 9, p. 201.

    Article  CAS  Google Scholar 

  10. Ilium, L., Pharmaceutical Research, 1998, vol. 9, p. 1326.

    Article  Google Scholar 

  11. Feng, J.J., Zhao, G., Xu, J.J., and Chen, H.Y., Anal. Biochem., 2005, vol. 342, p. 280.

    Article  CAS  Google Scholar 

  12. Liu, Y., Qu, X.H., Guo, H.W., Chen, H.J., Liu, B.F., and Dong, S.J., Biosensors Bioelectronics, 2006, vol. 21, p. 2195.

    Article  CAS  Google Scholar 

  13. Rivas, G.A., Miscoria, S.A., Desbrieres, J., and Barrera, G.D., Talanta, 2007, vol. 71, p. 270.

    Article  CAS  Google Scholar 

  14. Luo, H.X., Shi, Z.J., Li, N.Q., Gu, Z.N., and Zhuang, Q.K., Anal. Chem., 2001, vol. 73, p. 915.

    Article  CAS  Google Scholar 

  15. Lu, Y.F., Yin, Y.J., Wu, P., and Cai, C.X., Acta Phys. — Chim. Sinica, 2007, vol. 23, p. 5.

    Article  Google Scholar 

  16. Cai, C.X. and Chen, J., Anal. Biochem., 2004, vol. 325, p. 285.

    Article  CAS  Google Scholar 

  17. Liu, S.Q., Lin, B.P., Yang, X.D., and Zhang, Q.Q., J. Phys. Chem. B, 2007, vol. 111, p. 1182.

    Article  CAS  Google Scholar 

  18. O’Connell, M.J., Boul, P., Ericson, L.M., Huffman, C., Wang, Y.H., Haroz, E., Kuper, C., Tour, J., Ausman, K.D., and Smalley, R.E., Chem. Phys. Lett., 2001, vol. 342, p. 265.

    Article  CAS  Google Scholar 

  19. Zhao, H.T. and Ju, H.X., Anal. Biochem., 2006, vol. 350, p. 138.

    Article  CAS  Google Scholar 

  20. Murray, R.W., in Electroanalytical Chemistry, Bard, A.J., Ed., New York: Dekker, 1984, vol. 13, p. 191.

    Google Scholar 

  21. Wang, L. and Wang, E.K., Electrochem. Commun., 2004, vol. 6, p. 225.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, Wuhu, 241000, P.R. China

    L. P. Zhang, Y. Pan & Y. Z. Zhang

  2. Hennan Institute of Science and Technology, Xinxiang, 453003, P.R. China

    W. J. Gong

Authors
  1. L. P. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. J. Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Z. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. Z. Zhang.

Additional information

Published in Russian in Elektrokhimiya, 2008, Vol. 44, No. 11, pp. 1366–1376.

The text was submitted by the authors in English.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, L.P., Gong, W.J., Pan, Y. et al. Fabrication of multilayer-film-modified gold electrode composed of myoglobin, chitosan, and polyelectrolyte-wrapped multi-wall carbon nanotubes by layer-by-layer assembled technique and electrochemical catalysis for hydrogen peroxide and trichloroacetic acid. Russ J Electrochem 44, 1271–1279 (2008). https://doi.org/10.1134/S1023193508110128

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1023193508110128

Key words

Search

Navigation