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An Exhaustive Structural Scrutiny and Initial Functional Appraisal of the Yak SLC11A1 Gene

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Abstract

Animal diseases pose significant impediments to the flourishing of the animal husbandry sector, due to the role of solute transport protein family 11A1 (SLC11A1) gene on resistance or susceptibility to bacterial infections, rendering it a promising candidate gene for breeding disease-resistant animals. Here, we successfully cloned the coding region of the yak SLC11A1 gene. Comparative analysis against wild yaks and yellow cattle revealed the presence of 3 and 13 base variations, respectively. The N-terminal segment of the yak SLC11A1 gene’s coding region was expressed in a prokaryotic system, the protein exhibited inhibitory activity against Escherichia coli, a pathogen responsible for calf diarrhea. Furthermore, we successfully amplified a gene sequence encompassing a total length of 10 306 bp, and revealing the presence of 15 exons and 14 introns. Within the entire sequence, 14 SNPs were detected, and gave rise to 13 haplotypes. Furthermore, comparative analysis against cattle sequences (KR002419) unveiled two deletion sites, namely g.4511delACCCCACC and g.6410delCAGGT. This comprehensive examination provides insight into the structural attributes of both the coding region and the entire gene sequence, alongside an initial functional evaluation. It lays the foundation for the prospective utilization of the yak SLC11A1 gene as a molecular marker for disease resistance traits.

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DATA AVAILABILITY

The complete coding region and full-length gene sequence of the SLC11A1 gene in Zhongdian yak have been submitted to GenBank with registration numbers MH532569 and MK033321, respectively.

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Funding

This research was funded by Major Science and Technology Projects in Yunnan Province (grant no. 202202AE090005), and Doctoral Research Initiation Fund of Xinxiang University (grant no. 1366020172).

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Authors and Affiliations

  1. Faculty of Biological Engineering, Xinxiang University, 43003, Xinxiang, P. R. of China

    L. Wang

  2. Faculty of Animal Science and Technology, Yunnan Agricultural University, 65201, Kunming, P. R. of China

    B. Wang, Z. Gao, Y. Lu, W. Deng, D. Xi & Y. Chong

Authors
  1. L. Wang
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  2. B. Wang
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  3. Z. Gao
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  4. Y. Lu
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  5. W. Deng
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  6. D. Xi
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  7. Y. Chong
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Contributions

L. Wang and B. Wang contributed equally to this work.

Corresponding author

Correspondence to Y. Chong.

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The studies were carried out using biological material that was obtained from a slaughterhouse. Therefore, they did not need ethical approval.

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The authors of this work declare that they have no conflicts of interest.

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Wang, L., Wang, B., Gao, Z. et al. An Exhaustive Structural Scrutiny and Initial Functional Appraisal of the Yak SLC11A1 Gene. Russ J Genet 60, 493–502 (2024). https://doi.org/10.1134/S102279542404015X

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  • DOI: https://doi.org/10.1134/S102279542404015X

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