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Analysis of Genome Variability of Escherichia coli When Exposed to Ionizing Radiation

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Abstract

The study of changes in the genetic apparatus of biological systems due to the influence of physical factors contributes to understanding the mechanisms of adaptation. The article is devoted to the analysis of the genome of the modified Escherichia coli PL-6 variant obtained as a result of repeated and gradually increasing exposure to 60Co γ rays at the Researcher facility. The integrity of the genetic material of the studied bacterial cells was checked by electrophoresis in 1.7% agarose gel. For the analysis of genome modifications, primers were designed to amplify several loci characterized by homology in a variety of E. coli strains. On the basis of the indicators of the number and size of amplified products using each of the presented primer combinations in E. coli before and after gamma irradiation, a significant change in the genome was established.

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Funding

The work was carried out within the framework of the Strategic Academic Leadership Program of Kazan Federal University (priority 2030).

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Authors and Affiliations

  1. Federal Center for Toxicological, Radiation, and Biological Safety, 420075, Kazan, Russia

    M. Yu. Gallyamova, K. N. Vagin, N. M. Vasilevsky & N. I. Hammadov

  2. Kazan (Volga Region) Federal University, 420008, Kazan, Russia

    K. N. Vagin

Authors
  1. M. Yu. Gallyamova
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  2. K. N. Vagin
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  3. N. M. Vasilevsky
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  4. N. I. Hammadov
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Correspondence to K. N. Vagin.

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Gallyamova, M.Y., Vagin, K.N., Vasilevsky, N.M. et al. Analysis of Genome Variability of Escherichia coli When Exposed to Ionizing Radiation. Russ J Genet 60, 49–55 (2024). https://doi.org/10.1134/S1022795424010058

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