Skip to main content
SpringerLink
Log in

Regulation of CYP11A1 Gene by lncRNAs in Human Oocytes Obtained from Patients with Polycystic Ovaries

  • HUMAN GENETICS
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Polycystic ovary syndrome (PCOS) is a common premenopausal female disorder characterized by excess level of androgen and ovarian abnormalities. It affects 6 to 20% of women who have not undergone menopause, making it the most common metabolic and endocrine disorder affecting women of reproductive age. Every year, a large number of women seek treatment at reproductive clinics for PCOS-related issues. Infertility may be linked to altered gene expression levels in ovarian and oocyte samples. The effects of long non-coding RNAs (lncRNAs) on modifying gene expression in human oocytes obtained from patients with polycystic ovaries and patients without polycystic ovaries were investigated in this study. Thirteen metaphase II stage oocytes were obtained from individuals who applied to the Near East Hospital’s in vitro Fertilization (IVF) Clinic in Nicosia, Cyprus. The expression levels of three lncRNAs targeting the CYP11A1 gene were investigated in oocytes by using real-time polymerase chain reaction (PCR). The result of this study showed that there was no statistically significant (p > 0.05) change in the expression levels of RP11-573D15.8, RP11-156E8.1, and lnc-CYP11A1-1 between the patients with polycystic ovaries compared to patients without polycystic ovaries, respectively. The lncRNAs of RP11-573D15.8, RP11-156E8.1 and lnc-CYP11A1-1 were shown to regulate CYP11A1. We have previously shown that this gene is upregulated in oocytes obtained from patients of polycystic ovaries. Thus, lower expression levels of these lncRNAs were expected. However, the results of this study indicates that these lncRNAs do not have a regulatory role on the gene CYP11A1 in oocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.

Similar content being viewed by others

REFERENCES

  1. Lizneva, D., Atabiekov, I., and Azziz, R., Polycystic ovary syndrome, Encyclopedia of Reproduction, Skinner, M.K., Ed., Academic, 2018, 2nd ed., pp. 59—69. https://doi.org/10.1016/B978-0-12-801238-3.64517-8

  2. Escobar-Morreale, H.F., Defining PCOS: a syndrome with an intrinsic heterogeneous nature, Polycystic Ovary Syndrome, Elsevier, 2022, pp. 3—13. https://doi.org/10.1016/B978-0-12-823045-9.00012-2

  3. Jonard, S. and Dewailly, D., The follicular excess in polycystic ovaries, due to intra-ovarian hyperandrogenism, may be the main culprit for the follicular arrest, Hum. Reprod. Update, 2004, vol. 10, no. 2, pp. 107—117. https://doi.org/10.1093/humupd/dmh010

    Article  PubMed  Google Scholar 

  4. Chen, Y. and Fang, S., Potential genetic polymorphisms predicting polycystic ovary syndrome, Endocr. Connect., 2018, vol. 7, no. 5, pp. R187— R195. https://doi.org/10.1530/EC-18-0121

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Gambineri, A., Cecchetti, C., Altieri, P. et al., Secondary PCOS: well-defined causes, leading to the PCOS phenotype, Polycystic Ovary Syndrome, Elsevier, 2022, pp. 15—22. https://doi.org/10.1016/B978-0-12-823045-9.00008-0

  6. Joseph, S., Barai, R.S., Bhujbalrao, R., and Idicula-Thomas, S., PCOSKB: a knowledge base on genes, diseases, ontology terms and biochemical pathways associated with polycystic ovary syndrome, Nucleic Acids Res., 2016, vol. 44, no. D1, pp. D1032—D1035. https://doi.org/10.1093/nar/gkv1146

    Article  CAS  PubMed  Google Scholar 

  7. Al-Omar, Z., Ozbakir, B., and Tulay, P., Differential expression of genes involved in steroidogenesis pathway in human oocytes obtained from patients with polycystic ovaries, J. Reprod. Immunol., 2020, vol. 142, р. 103191. https://doi.org/10.1016/j.jri.2020.103191

    Article  CAS  PubMed  Google Scholar 

  8. Mohr, A.M., Sahoo, P.K., Muthuraj, P.G., et al., Epigenetics, noncoding RNAs, and gene expression, Compr. Foodomics, 2020, pp. 258—272. https://doi.org/10.1016/B978-0-08-100596-5.22707-8

  9. Tu, M., Wu, Y., Mu, L., and Zhang, D., Long non-coding RNAs: novel players in the pathogenesis of polycystic ovary syndrome, Ann. Transl. Med., 2021, vol. 9, no. 2, p.173. https://doi.org/10.21037/atm-20-5044

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Fukunaga, T., Iwakiri, J., Ono, Y., and Hamada, M., LncRRIsearch: a web server for lncRNA—RNA interaction prediction integrated with tissue-specific expression and subcellular localization data, Front. Genet., 2019, vol. 10. https://doi.org/10.3389/fgene.2019.00462

  11. Bouckenheimer, J., Fauque, P., Lecellier, C.H., et al., Differential long non-coding RNA expression profiles in human oocytes and cumulus cells, Sci. Rep., 2018, vol. 8, р. 2202. https://doi.org/10.1038/s41598-018-20727-0

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

Author information

Authors and Affiliations

  1. Department of Medical Genetics, Faculty of Medicine, Near East University, 99138, Nicosia, Cyprus

    M. Aktan, H. Aytaçoğlu & P. Tulay

  2. Department of Obstetrics and Gynecology, Faculty of Medicine, Near East University, 99138, Nicosia, Cyprus

    B. Özbakır

  3. Near East University, DESAM Research Institute, 99138, Nicosia, Cyprus

    B. Özbakır & P. Tulay

  4. Near East University, Center of Excellence, Genetics and Cancer Diagnosis-Research Center, Nicosia, Cyprus

    P. Tulay

Authors
  1. M. Aktan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Aytaçoğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Özbakır
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Tulay
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Mehmet Aktan performed the molecular parts of the experiments, analyzed the data including the statistical analysis, and wrote the manuscript.

Hakan Aytacoglu performed the molecular parts of the experiments, analyzed the data including the statistical analysis, and wrote the manuscript.

Burcu Ozbakir was involved in diagnosis of the patients and sample collection.

Pinar Tulay was the supervisor of the project and involved in the study design, and analysis and approved the final version of the manuscript.

Corresponding author

Correspondence to P. Tulay.

Ethics declarations

Conflict of interest. The authors declare that they have no conflicts of interest.

Statement of compliance with standards of research involving humans as subjects. This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Near East University Institutional Review Board (YDU/2019/75-920).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Aktan, M., Aytaçoğlu, H., Özbakır, B. et al. Regulation of CYP11A1 Gene by lncRNAs in Human Oocytes Obtained from Patients with Polycystic Ovaries. Russ J Genet 59 (Suppl 1), 106–109 (2023). https://doi.org/10.1134/S102279542313001X

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S102279542313001X

Keywords:

Search

Navigation