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Effect of Chronic Radiation Exposure on Human MicroRNA Expression

  • HUMAN GENETICS
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Abstract

An estimate of relative expression of mature microRNAs assessed in peripheral blood cells in persons chronically exposed to low-dose radiation in the long-term period was performed. The study included people exposed in the 1950s on the Techa River (Southern Urals, Russia). The cumulative red bone marrow doses (RBM) of the persons in the main study group (33 persons) ranged from 77.7 to 2869.8 mGy (mean value of 698.5 mGy). The comparison group consisted of 30 people with RBM dose not exceeding 70 mGy over their lifetime. Reverse transcription of RNA samples was performed using specific stem-loop primers (“stem-loop”). Changes in the relative content of microRNAs were assessed by real-time PCR on the CFX96 detection system (BioRad, United States). Statistical analysis of the results was performed using the Mann–Whitney U criterion. A significant increase in hsa-miR-125b, hsa-miR-181a, and hsa-miR-16-5p content was detected in exposed people after 60 years, and their expression was found to depend on the RBM dose.

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Funding

This work was financed within the framework of the federal target program “Ensuring Nuclear and Radiation Safety for 2016–2020 and for the Period up to 2030.”

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Authors and Affiliations

  1. Urals Research Center for Radiation Medicine, 454141, Chelyabinsk, Russia

    M. A. Yanishevskaya, E. A. Blinova & A. V. Akleyev

  2. Chelyabinsk State University, 454001, Chelyabinsk, Russia

    E. A. Blinova & A. V. Akleyev

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  1. M. A. Yanishevskaya
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  2. E. A. Blinova
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Correspondence to M. A. Yanishevskaya.

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Statement of compliance with standards of research involving humans as subjects. All procedures performed in research with human participants comply with the ethical standards of the institutional and/or national committee on research ethics and the 1964 Declaration of Helsinki and its subsequent amendments or comparable ethical standards.

Informed consent was obtained from each of the participants included in the study.

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Yanishevskaya, M.A., Blinova, E.A. & Akleyev, A.V. Effect of Chronic Radiation Exposure on Human MicroRNA Expression. Russ J Genet 59, 1050–1057 (2023). https://doi.org/10.1134/S1022795423100150

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