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Genetic Diversity and Population Structure of Traditional Chinese Herb Radix bupleuri Resources Using Genome-Wide SNPs through Genotyping-by-Sequencing

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Abstract

Radix bupleuri, is an important traditional Chinese medicine resource. The lack of genetic molecular markers of Radix bupleuri turned to be an obstacle for genetic analysis and accessions identification. In this study, SNP loci were detected based on genotyping-by-sequencing (GBS) and the genetic diversity of 39 Bupleurum varieties were examined. A total of 25.1 Gb of data was obtained by sequencing, with an average of 0.64 Gb per sample. After screening, 83 898 high-quality SNPs were obtained. The 39 accessions could be divided into three major groups based on population structure analysis, Neighbor-joining clustering and principal component analysis. The average observed heterozygosity and expected heterozygosity of Bupleurum populations were 0.24 and 0.17, respectively, indicating that Bupleurum populations from five different provinces had a low level of genetic diversity. Population nucleotide diversity analysis and analysis of molecular variance showed that the percentage of intrapopulation variation was 92.13%, while the percentage of interpopulation variation was only 7.87%. There was relative aggregation of Bupleurum samples with the same geographical origin, but the division of population structure was not completely correlated with sample origin. The results showed that the genetic diversity of the materials was narrow. This provides a good basis for the genetic breeding and protection of species diversity of Bupleurum.

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ACKNOWLEDGMENTS

We would like to thank Springer Nature for providing linguistic assistance for manuscript preparation.

Funding

This work was supported by the Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (2060302), the State Key Laboratory of Pharmaceutical New-tech for Chinese Medicine (SKL2020M0302) and the Talent training project funded by the central government to support the reform and development of local colleges and Universities (ZYRCB2021008).

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    Authors and Affiliations

    1. School of Pharmacy, Heilongjiang University of Chinese Medicine, 150000, Harbin, Heilongjiang, China

      M. Jiang, S. Yan, W. C. Ren, N. N. Xing, H. Y. Li, M. Q. Liu, X. B. Liu & W. Ma

    2. Department of Science and Technology, Qiqihar Medical University, 161006, Qiqihar, Heilongjiang, China

      M. Jiang

    3. School of Forestry, Northeast Forestry University, 150000, Harbin, Heilongjiang, China

      M. Q. Zhang

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    M. Jiang, S. Yan, W.C. Ren, and N.N. Xing contributed equally to this work.

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    Jiang, M., Yan, S., Ren, W.C. et al. Genetic Diversity and Population Structure of Traditional Chinese Herb Radix bupleuri Resources Using Genome-Wide SNPs through Genotyping-by-Sequencing. Russ J Genet 58, 1485–1492 (2022). https://doi.org/10.1134/S1022795422120055

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