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Molecular Phylogeny of Russian Species of the Genus Spiraea (Rosaceae) according to the Nucleotide Variability of the ITS Nuclear rDNA Region

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Abstract

The results of studying the variability of sequences of the ITS rDNA region for 17 species of the genus Spiraea (Magnoliópsida: Rosaceae) occurring within the Russian part of the ranges and in adjacent territories are presented. The results demonstrate the presence of single-nucleotide substitutions (transitions and transversions) and deletions/insertions, including those specific at the level of subgenera, sections, cycles, and series. The variability in the ITS1 intergenic spacer is higher than in ITS2. The high importance of ITS as a molecular marker in the resolution of phylogenetic relationships in the genus Spiraea is emphasized. Closely related Spiraea species usually differ in ITS sequences, but the polymorphic section Chamaedryon has the greatest difficulties in identifying taxa. Phylogenetic analysis carried out in various ways (ML, MP, and BI) confirms the monophyletic origin of the genus Spiraea and the ancient sections Spiraea and Calospira. A hypothesis is formulated about the paraphyletic nature of the section Chamaedryon, which more evolutionarily young, diverse, and richest in the number of species. It is assumed that the paraphyly of the Chamaedryon section is associated with divergence of traits and subsequent isolation of species along with multiple migrations under the influence of contrasting environmental conditions.

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ACKNOWLEDGMENTS

The authors thank E.A. Bondarevich (Chita State Academy of Medicine, Chita) for the assistance in collecting Spiraea samples in Zabaykalsky krai and M.A. Polezhaeva for kindly providing herbarium samples from Sverdlovsk oblast and Chelyabinsk oblast (Institute of Plant and Animal Ecology, Yekaterinburg).

Funding

This study was supported by the Russian Foundation for Basic Research (project no. 15-04-03093) as well as within the framework of the state task of the Ministry of Science and Higher Education of the Russian Federation for Vavilov Institute of General Genetics of the Russian Academy of Sciences (no. 0112-2019-0001).

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  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia

    T. A. Poliakova, A. V. Shatokhina & D. V. Politov

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  1. T. A. Poliakova
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  2. A. V. Shatokhina
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Correspondence to T. A. Poliakova.

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Translated by M. Novikova

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Poliakova, T.A., Shatokhina, A.V. & Politov, D.V. Molecular Phylogeny of Russian Species of the Genus Spiraea (Rosaceae) according to the Nucleotide Variability of the ITS Nuclear rDNA Region. Russ J Genet 58, 1297–1305 (2022). https://doi.org/10.1134/S1022795422110084

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