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New Cattle Genotyping System Based on DNA Microarray Technology

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Abstract

A prototyping model of the DNA microarray system for identification of genetic markers related to productivity, determination of cattle breed purity, and identification of cattle monogenic diseases was developed. A method for immobilizing oligonucleotides on a polymer support, their fixation using ultraviolet irradiation, and DNA hybridization to them, followed by labeling and genotype determination, was developed. Genotyping of two genes encoding milk caseins, i.e., CSN3 kappa-casein gene and CSN2 beta-casein gene, in the Aberdeen Angus breed using real-time PCR and the developed DNA microarray showed identical results. The potential of DNA microarray technology, its principle, and the possibilities of application to animal husbandry for genetic breeding work (monitoring, determining the genetic potential and diversity in breeds and populations of cattle, and agrobiodiversity in general) are considered.

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Funding

This study was supported by the Russian Science Foundation (grant no. 19-76-20061) (http://rscf.ru).

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia

    Yu. A. Stolpovsky, S. B. Kuznetsov & E. V. Solodneva

  2. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Sciences, 119435, Moscow, Russia

    I. D. Shumov

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  1. Yu. A. Stolpovsky
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  2. S. B. Kuznetsov
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  3. E. V. Solodneva
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  4. I. D. Shumov
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Correspondence to Yu. A. Stolpovsky.

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Translated by N. Maleeva

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Stolpovsky, Y.A., Kuznetsov, S.B., Solodneva, E.V. et al. New Cattle Genotyping System Based on DNA Microarray Technology. Russ J Genet 58, 885–898 (2022). https://doi.org/10.1134/S1022795422080099

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