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Tissue Specificity of the AqE Gene Activity in the Yellow Croaker Larimichthys crocea

  • MOLECULAR GENETICS
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Abstract

The study of the diversity of metabolic pathways is an important aspect for understanding the evolutionary relationships between metabolic pathways and their biochemical precursors. Recently, researchers described a sulfolactate dehydrogenase-like protein encoded in eukaryotes by the AqE gene, which remains highly conserved in teleosts. However, the metabolic role of this enzyme is still unknown. In the present work, we studied the transcriptional activity of the AqE gene, as well as other genes associated with energy exchange in the large yellow croaker Larimichthys crocea. The quantitative analysis of expression showed the tissue specificity of the AqE gene activity in the yellow croaker. The gene is active in the liver, skin, and gills. The analysis of gene expression in various organs and under the influence of stressful conditions suggests that the enzyme encoded by the AqE gene is involved in the malate-aspartate shuttle or in the excretion of the final metabolites (sulfolactate) from the organism.

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Funding

The work was supported by the state assignment of the Federal Research Center Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, “Functional, Metabolic, and Toxicological Aspects of the Existence of Hydrobionts and Their Populations in Biotopes with Different Physicochemical Regimes,” state registration number 121041400077-1, and by the Russian Foundation for Basic Research and the city of Sevastopol, scientific project no. 20-44-920006.

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  1. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, 299011, Sevastopol, Russia

    L. V. Puzakova & M. V. Puzakov

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  1. L. V. Puzakova
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  2. M. V. Puzakov
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Correspondence to L. V. Puzakova.

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Translated by A. Lisenkova

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Puzakova, L.V., Puzakov, M.V. Tissue Specificity of the AqE Gene Activity in the Yellow Croaker Larimichthys crocea. Russ J Genet 58, 538–546 (2022). https://doi.org/10.1134/S1022795422050076

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