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Buccal Micronucleus Cytome Assay for the Evaluation of Cytogenetic Status of Healthcare Professionals Contacting with Anti-Cancer Drugs

  • GENERAL GENETICS
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Abstract

Healthcare professionals of chemotherapy departments are in a regular contact with anticancer drugs that have genotoxic properties. The analysis of the cytogenetic status of healthcare professionals (54 individuals) was for the first time carried out using a new promising noninvasive method, buccal micronucleus cytome assay with the calculation of integral indices. The effect was estimated with different duration of exposure to chemotherapy drugs (after an employee’s vacation, one month after vacation, and before vacation). Statistically significant increases in almost all studied indices of cytogenetic damages, proliferation, and apoptosis as compared with the control group of office staff (47 individuals) were detected. Among healthcare professionals, an increase in the portion of individuals with an increased level of cytogenetic abnormalities with the maximum exposure duration from 24 to 38% (11% in the control) was established. Among cancer patients (9 individuals), the portion of individuals with a high level of cytogenetic abnormalities was 56% before the next course of chemotherapy and 87% after it.

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Authors and Affiliations

  1. Blokhin National Medical Research Center of Oncology, 115478, Moscow, Russia

    K. I. Kirsanov, E. A. Lesovaya, E. M. Zhidkova, O. A. Vlasova, A. V. Osipova, E. S. Lylova, D. D. Grigorieva, E. M. Kulakova, L. G. Solenova, G. A. Belitsky, I. N. Mikhaylova & M. G. Yakubovskaya

  2. Peoples’ Friendship University of Russia, 117198, Moscow, Russia

    K. I. Kirsanov

  3. Burnazyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 123182, Moscow, Russia

    L. P. Sycheva

  4. Pavlov Ryazan State Medical University, 390026, Ryazan, Russia

    E. A. Lesovaya

  5. Loginov Moscow Clinical Scientific Center, 111123, Moscow, Russia

    A. V. Osipova

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  1. K. I. Kirsanov
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  2. L. P. Sycheva
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  3. E. A. Lesovaya
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  7. E. S. Lylova
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  8. D. D. Grigorieva
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  9. E. M. Kulakova
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  10. L. G. Solenova
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Corresponding author

Correspondence to L. P. Sycheva.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in the study involving human participants are in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent was obtained from each of the participants involved in the study.

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Translated by A. Barkhash

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Kirsanov, K.I., Sycheva, L.P., Lesovaya, E.A. et al. Buccal Micronucleus Cytome Assay for the Evaluation of Cytogenetic Status of Healthcare Professionals Contacting with Anti-Cancer Drugs. Russ J Genet 58, 528–537 (2022). https://doi.org/10.1134/S1022795422050052

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  • DOI: https://doi.org/10.1134/S1022795422050052

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