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Genetic Diversity of Four Species of the Genus Astragalus L. Section Cenantrum Bunge (Fabaceae) in the Asian Part of Russia according to the Data of trnL–trnF Chloroplast DNA Variability and Isozyme Markers

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Abstract

The genetic diversity and phylogenetic relationships of four species of the genus Astragalus of the section Cenantrum, A. frigidus, A. mongholicus, A. penduliflorus, and A. sericeocanus from the Asian part of Russia, were studied according to the variability of trnL-trnF cpDNA and isozyme markers. For each species, specific sets of chloroplast haplotypes, alleles, and isoenzyme frequencies were identified. According to cpDNA data, the studied populations are characterized by low haplotype (h varies from 0.095 to 0.333) and nucleotide (π varies from 0.00019 to 0.00378) diversity. Most of the populations (20 out of 27) were monomorphic. According to the data of isoenzyme analysis, the indicators of polymorphism for three species (with the exception of the monomorphic population of A. penduliflorus) have rather high values. For a species on average, the polymorphism (P95) varies from 55.5 to 85.5%, the number of alleles per locus (A) is from 1.8 to 2.7, and the observed heterozygosity (Ho) is from 0.134 to 0.215. The geographic confinement of haplotypes and polymorphic populations of A. frigidus and A. mongholicus was revealed in Altai and Baikal Siberia. These localities are probably situated at the intersection of migration routes or refugia.

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ACKNOWLEDGMENTS

We are grateful to our colleagues A.V. Verkhozina, E.V. Zhmud’, M.A. Polezhayeva, D.V. Sandanov, D.G. Chimitov and E.G. Filippov for their assistance in collecting research material and data analysis.

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Authors and Affiliations

  1. Sakhalin Branch of the Botanical Garden-Institute, Far Eastern Branch, Russian Academy of Sciences, 693023, Yuzhno-Sakhalinsk, Russia

    O. S. Korchagina

  2. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, 664033, Irkutsk, Russia

    D. A. Krivenko

  3. Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences, 620144, Yekaterinburg, Russia

    A. Yu. Belyaev

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  1. O. S. Korchagina
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  2. D. A. Krivenko
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  3. A. Yu. Belyaev
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Correspondence to O. S. Korchagina or D. A. Krivenko.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Makeeva

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Korchagina, O.S., Krivenko, D.A. & Belyaev, A.Y. Genetic Diversity of Four Species of the Genus Astragalus L. Section Cenantrum Bunge (Fabaceae) in the Asian Part of Russia according to the Data of trnL–trnF Chloroplast DNA Variability and Isozyme Markers. Russ J Genet 58, 27–35 (2022). https://doi.org/10.1134/S1022795422010057

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