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Invasive Populations of Leafhopper Species Metcalfa pruinosa (Hemiptera: Flatidae) and Arboridia kakogawana (Hemiptera: Cicadellidae) Are Not Infected with Intracellular Symbiotic Bacteria

  • ANIMAL GENETICS
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Abstract

The appearance and rapid spread of new invasive leafhopper species Metcalfa pruinosa (Say, 1830) and Arboridia kakogawana (Matsumura, 1932) in Russia requires studying both the causes of the invasion and consequences of invasion for viticulture. Of particular interest is the study of the possible role of new species of leafhoppers in the spread of pathogenic bacteria of grape phytoplasmosis. At present, these issues are poorly studied. We characterized the variability of the BOLD fragment of the mitochondrial COI gene in the population samples of M. pruinosa and A. kakogawana from the populations of Crimea and the Black Sea coast of the Caucasus. With the help of PCR for specific genes, we searched for leafhoppers infected with bacteria of phytoplasmosis of grapes from the group of stolbur (Candidatus Phytoplasma solani) and intracellular symbiotic bacteria: Wolbachia, Rickettsia, and Spiroplasma. We found M. pruinosa only on the Black Sea coast of the Caucasus. The M. pruinosa populations are close to monomorphism. We found A. kakogawana in both Crimea and the Caucasus region. We have identified two new forms of this species in Russia. We have not found pathogenic bacteria of phytoplasmosis of grapes and intracellular symbiotic bacteria in studied leafhopper populations.

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REFERENCES

  1. Metcalf, Z.P. and Bruner, S.C., Cuban Flatidae with new species from adjacent regions, Ann. Entomol. Soc. Am., 1948, vol. 41, pp. 63–97.

    Article  Google Scholar 

  2. Zangheri, S. and Donadini, P., Comparsa nel Veneto di un Omottero nearctico: Metcalfa pruinosa Say (Homoptera, Flatidae), Redia, 1980, vol. 63, pp. 301–306.

    Google Scholar 

  3. Jermini, M., Bonavia, M., Brunetti, R., Mauri, G., and Cavalli, V., Metcalfa pruinosa Say, Hyphantria cunea (Drury) and Dichelomyia oenophila Haimah: three entomological curiosities or a new phytosanitary problem for Ticino and Switzerland?, Switz. Rev. Vitic., Arboric., Hortic., 1995, vol. 27, no. 1, pp. 57–63.

    Google Scholar 

  4. Lauterer, P., Citrus flatid planthopper Metcalfa pruinosa (Hemiptera: Flatidae), a new pest of ornamental horticulture in the Czech Republic, Plant Prot. Sci., 2002, vol. 38, pp. 145–148.

    Article  Google Scholar 

  5. Gogan, A.I. and Grozea, A.M., Virteiu Metcalfa pruinosa Say (Insecta: Homoptera: Flatidae)—first occurrence in western part of Romania, Res. J. Agric. Sci., 2010, vol. 42, no. 4, pp. 63–67.

    Google Scholar 

  6. Gnezdilov, V.M. and Sugonyaev, T.S., First record of Metcalfa pruinosa (Homoptera: Fulgoroidea: Flatidae) from Russia, Zoosyst. Ross., 2009, vol. 18, no. 2b, pp. 260–261.

    Article  Google Scholar 

  7. Balakhnina, I.V., Pastarnak, I.N., and Gnezdilov, V.M., Monitoring and control of Metcalfa pruinosa (Say) (Hemipteran, Auchenorrhyncha: Flatidae) numbers in the Krasnodar Krai, Entomol. Obozr., 2014, vol. 3, pp. 3–4.

    Google Scholar 

  8. Preda, C. and Skolka, M., Range expansion of Metcalfa pruinosa (Homoptera: Fulgoroidea) in Southeastern Europe, Ecol. Balk., 2011, vol. 3, no. 1, pp. 79–87.

    Google Scholar 

  9. Zamotailov, A.S., Shchurov, V.I., and Belyi, I.A., White leafhopper—a new threat to agriculture and forestry in the south of Russia, Zashch. Karantin Rast., 2012, no. 4, pp. 46–47.

  10. Matsumura, S., A revision of the Palaearctic and oriental typhlocybid—genera with descriptions of new species and new genera, Ins. Mats., 1932, vol. 6, no. 3, pp. 93–120.

    Google Scholar 

  11. Anufriev, G.A. and Emel’yanov, A.F., Opredelitel’ nasekomykh Dal’nego Vostoka SSSR (Keys to Insects of the Soviet Far East), vol. II: Ravnokrylye i poluzhestkrylye (Homoptera and Hemiptera), Leningrad: Nauka, 1988.

  12. Sugonyaev, E.S., Gnezdilov, V.M., and Yakovuk, V.A., New potential pest of grapes, Zashch. Karantin Rast., 2004, vol. 7, p. 35.

    Google Scholar 

  13. Balakhnina, I.V., Sugonyaev, E.S., and Yakovuk, V.A., Japanese grape leafhopper—new potentially dangerous vine pest in the North Caucasus, Zashch. Karantin Rast., 2009, vol. 12, pp. 33–34.

    Google Scholar 

  14. Radionovskaya, Ya.E. and Didenko, L.V., Evaluation of the harmfulness of a new phytophage—the Japanese grape leafhopper Arboridia kakogawana mats—in the vineyards of Crimea, Byull. Gos. Nikitsk. Bot. Sada, 2015, vol. 114, pp. 61–68.

    Google Scholar 

  15. Gnezdilov, V.M., Sugonyaev, E.S., and Artokhin, R.S., Arboridia kakogawana (Matsumura) (Hemiptera Cicadellidae Typhlocybinae)—a new pest of grapevine in Southern Russia, REDIA, 2008, vol. 1, pp. 51–54.

    Google Scholar 

  16. Tramontini, S., Delbianco, A., and Vos, S., Pest survey card on flavescence dorée phytoplasma and its vector Scaphoideus titanus, EFSA Supporting Publ., 2020, vol. 17, no. 8, pp. 1–36. https://doi.org/10.2903/sp.efsa.2020.EN-1909

    Article  Google Scholar 

  17. Sforza, R., Clair, D., Daire, X., Larrue, J., and Boudon-Padieu, E., The role of Hyalesthes obsoletus (Hemiptera: Cixiidae) in the occurrence of Bois Noir of grapevines in France, J. Phytopathol., 1998, vol. 146, pp. 549–556. https://doi.org/10.1111/j.1439-0434.1998.tb04753.x

    Article  Google Scholar 

  18. Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 1982.

    Google Scholar 

  19. Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek, R., DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates, Mol. Mar. Biol. Biotechnol., 1994, vol. 3, pp. 294–299.

    CAS  PubMed  Google Scholar 

  20. Baldo, L., Hotopp, J.C.D., Jolley, K.A., et al., Multilocus sequence typing system for the endosymbiont Wolbachia pipientis, Appl. Environ. Microbiol., 2006, vol. 72, no. 11, pp. 7098–7110.

    Article  CAS  Google Scholar 

  21. Goryacheva, I.I., Blekhman, A.V., Andrianov, B.V., et al., Genotypic diversity of Wolbachia pipientis in native and invasive Harmonia axyridis Pall., 1773 (Coleoptera, Coccinellidae) populations, Russ. J. Genet., 2015, vol. 51, no. 8, pp. 731–736. https://doi.org/10.1134/S1022795415080037

    Article  CAS  Google Scholar 

  22. Goryacheva, I., Blekhman, A., Andrianov, B., and Zakharov, I., Heritable bacterial endosymbionts in native and invasive populations of Harmonia axyridis, Biol. Invasions, 2017, vol. 19, no. 2, pp. 493–502. https://doi.org/10.1007/s10530-016-1298-8

    Article  Google Scholar 

  23. Majerus, T.M.O., Graf von der Schulenburg, J.H., Majerus, M.E.N., and Hurst, G.D., Molecular identification of a male-killing agent in the ladybird Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), Insect Mol. Biol., 1999, vol. 8, pp. 551–555. https://doi.org/10.1046/j.1365-2583.1999.00151.x

    Article  CAS  PubMed  Google Scholar 

  24. Lorenz, K.H., Schneider, B., Ahrens, U., and Seemuller, E., Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA, Phytopathology, 1995, vol. 85, pp. 771–776.

    Article  CAS  Google Scholar 

  25. Kumar, S., Stecher, G., and Tamura, K., MEGA7: molecular evolutionary genetics analysis. Version 7.0 for bigger datasets, Mol. Biol. Evol., 2016, vol. 33, no. 7, pp. 1870–1874. https://doi.org/10.1093/molbev/msw054

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Hebert, P.D., Cywinska, A., Ball, S.L., and de Waard, J.R., Biological identifications through DNA barcodes, Proc. Biol. Sci., 2003, vol. 270, no. 1512, pp. 313–321. https://doi.org/10.1098/rspb.2002.2218

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Aleinikova, N.V. and Radionovskaya, Ya.E., Introduced planting material is a source of phytoplasmic infection in the vineyards of Crimea, Zashch. Karantin Rast., 2015, no. 9, pp. 31–33.

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Funding

This work was financially supported by the Russian Science Foundation, project 16-16-00079.

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    Authors and Affiliations

    1. Vavilov Institute of General Genetics Russian Academy of Sciences, 119991, Moscow, Russia

      B. V. Andrianov, D. A. Romanov & E. A. Matveykina

    2. All-Russian National Research Institute of Viticulture and Winemaking Magarach, Russian Academy of Sciences, 298600, Yalta, Russia

      E. A. Matveykina

    3. Moscow Region State University, 141014, Mytishi, Moscow oblast, Russia

      B. V. Andrianov

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    1. B. V. Andrianov
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    3. E. A. Matveykina
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    Correspondence to B. V. Andrianov.

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    Andrianov, B.V., Romanov, D.A. & Matveykina, E.A. Invasive Populations of Leafhopper Species Metcalfa pruinosa (Hemiptera: Flatidae) and Arboridia kakogawana (Hemiptera: Cicadellidae) Are Not Infected with Intracellular Symbiotic Bacteria. Russ J Genet 57, 1164–1169 (2021). https://doi.org/10.1134/S1022795421100021

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