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Structure and Formation Mechanisms of Polymorphism of Hordein-Coding Loci in Domesticated Barley (Hordeum vulgare L.)

  • PLANT GENETICS
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Abstract

Hordein polymorphism in 2244 barley accessions was studied using starch gel electrophoresis. A total of 1197 old local accessions from 22 countries located in the main centers of barley diversity and the bordering regions, 428 varieties cultivated in the territory of the former Soviet Union and Russian Federation from 1929 to 2019, and 619 varieties from Europe, Asia, America, and Africa were analyzed. For the hordeins encoded by the Hrd A locus, 156 variants of blocks of components were identified; 271 variants were detected for the Hrd B locus; and 5 variants were detected for the Hrd F locus. On the basis of the published data on the molecular mechanisms of hordein polymorphism formation and analysis of artificial mutants at the hordein-coding loci, we identified groups, or families, of phenotypically similar component blocks among A- and B-hordein variants. For HRD A, 12 families (AI–AXII) were identified, and for HRG B, 17 families (BI–BXVII) were identified. The families differ significantly in the number of block variants: for HRD A, from 5 (AXII) to 60 (AI); for HRD B, from 3 (BXVII) to 41 (BXII). It is suggested that the families with the largest number of A- and B-hordein variants are the most ancient in domesticated barley. The mosaic structure and irregular distribution of frequencies of HRD A and HRD B family variants in local populations of different countries was shown. We suggested that the emergence of the HRD A and HRD B families was due to the spontaneous hybridization between H. vulgare and H. spontaneum during the spreading of domesticated barley. It is concluded that hordein polymorphism in domesticated barley resulted from spontaneous hybridization between the cultivated barley with a wild ancestor and accumulation of mutations in the genes at Hrd A and Hrd B loci in H. vulgare.

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REFERENCES

  1. Dakir, El-H., Ruiz, M.-L., Garcia, P., et al., Genetic variability evaluation in Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDs, Genet. Res. Crop Evol., 2002, vol. 49, pp. 619—631.

  2. Sozinov, A.A., Netsvetaev, V.P., Grigoryan, E.M., and Obraztsov, I.S., Mapping Hrd loci in barley (Hordeum vulgare L. emend. Vav. et Bach.), Genetica (Moscow), 1978, vol. 14, no. 9, pp. 1610—1619.

    Google Scholar 

  3. Pomortsev, A.A., Netsvetaev, V.P., Poperelya F.A., et al., Identification of sixth locus that controls the synthesis of hordein in winter barley, Dokl. Vses. Akad. S-kh. Nauk im. Lenina, 1983, no. 1, pp. 7—11.

  4. Netsvetaev, V.P., Obraztsov, I.S., and Sozinov, A.A., Mapping of the Hrd G locus on chromosome 5 of barley, Molekulyarnye mekhanizmy geneticheskikh protsessov (Molecular Mechanisms of Genetic Processes) (Proc. V All-Union Symp.), Moscow: Nauka, 1983, p. 110.

  5. Shewry, P.R., Faulks, A.J., Pickering, R.A., et al., The genetic analysis of barley storage proteins, Heredity, 1980, vol. 44, pp. 383—389.

    Article  CAS  Google Scholar 

  6. Blake, T.K., Ullrich, S.E., and Nilan, R.A., Mapping of the Hor-3 locus encoding D hordein in barley, Theor. Appl. Genet., 1982, vol. 63, pp. 367—372.

    Article  CAS  Google Scholar 

  7. Pomortsev, A.A., Netsvetaev, V.P., and Sozinov, A.A., Polymorphism of cultivated barley (Hordeum vulgare L.) for hordeins, Genetica (Moscow), 1985, vol. 21, no. 4, pp. 629—639.

    Google Scholar 

  8. Miflin, B.J. and Shewry, P.R., An introduction to the extraction and characterisation of barley and maize proteins, in Techniques for the Separation of Barley and Maize Proteins, Miflin, B.J. and Shewry, P.R., Eds., Luxembourg: EEC, 1982, pp. 13—25.

    Google Scholar 

  9. Ladogina, M.P., Pomortsev, A.A., Netsvetaev, V.P., et al., Inheritance and the genetic control of glutelin components in barley (Hordeum vulgare L.), Genetica (Moscow), 1988, vol. 24, no. 2, pp. 316—323.

    CAS  Google Scholar 

  10. Shewry, P.R. and Parmar, S., The Hrd F (Hor 5) locus encodes γ-type hordeins, Barley Genet. Newslett., 1987, vol. 17, pp. 32—34.

    Google Scholar 

  11. Shewry, P.R., Parmar, S., Franklin, J., et al., Mapping and biochemical analysis of Hor 4 (Hrd G), a second locus encoding B hordein seed proteins in barley (Hordeum vulgare L.), Genet. Res. Camb., 1988, vol. 51, pp. 5—12.

    Article  CAS  Google Scholar 

  12. Sozinov, A.A. and Poperelya, F.A., Metodika vertikal’nogo diskovogo elektroforeza v krakhmal’nom gele i geneticheskii printsip klassifikatsii gliadinov (A Procedure of Vertical Disk Electrophoresis in Starch Gel and the Genetic Principle of Gliadin Classification), Odessa, 1978.

  13. Pomortsev, A.A. and Lyalina, E.V., Assessment of barley seeds cultivar identity and purity by means of electrophoretic analysis of storage proteins, in Metodicheskoe posobie k praktikumu Belkovye markery dlya geneticheskoi pasportizatsii i uluchsheniya genomov rastenii khozyaistvenno tsennykh vidov” (Laboratory Methodic Guide to the Practicum “Protein Markers for Genetic Certification and Improvement of Plant Genomes of Economically Valuable Species”), Moscow: Tsifrovichok, 2011.

  14. Kreis, M., Shewry, P.R., Forde, B.G., et al., Structure and evolution of seed storage proteins and their genes with particular reference of those of wheat, barley and rye, Oxford Surv. Plant Mol. Cell Biol., 1985, no. 2, pp. 253—317.

  15. Shewry, P.R., Bunce, N.A.C., Kreis, M., et al., Polymorphism at the Hor 1 locus of barley (Hordeum vulgare L.), Biochem. Genet., 1985, vol. 23, nos. 5—6, pp. 391—403.

    Article  CAS  Google Scholar 

  16. Bunce, N.A.C., Forde, B.G., Kreis, M., et al., DNA restriction fragment length polymorphism of hordein loci: application to identifying and fingerprinting barley cultivars, Seed Sci. Techn., 1986, vol. 14, pp. 419—429.

    CAS  Google Scholar 

  17. Sayanova, O.V., Mekhedov, S.L., Burkitbaev, E.M., et al., Structural organization of barley C-hordein genes, Genetica (Moscow), 1994, vol. 30, no. 6, pp. 749—755.

    CAS  Google Scholar 

  18. Sayanova, O., Pomortsev, A., Ladogina, M., et al., Analysis of C-hordein deficient mutant of barley, Barley Genet. Newslett., 1992, vol. 22, pp. 53—56.

    Google Scholar 

  19. Kanazin, V., Ananiev, E., and Blake, T., Variability among members of Hor-2 multigene family, Genome, 1993, vol. 26, pp. 397—403.

    Article  Google Scholar 

  20. Shewry, P.R., Parmar, S., and Franklin, J., Recombinant within the Hor 2 locus, Barley Genet. Newslett., 1988, vol. 18, pp. 31—33.

    Google Scholar 

  21. Wiberg, A., Genetical studies of spontaneous sources of resistance to powdery mildew in barley, Heredity, 1974, vol. 77, pp. 89—148.

    Article  CAS  Google Scholar 

  22. Mozeman, J.G., Nevo, E., and Zohary, D., Resistance of Hordeum spontaneum collected in Israel to infection with Erysiphe graminis hordei, Crop Sci., 1983, vol. 23, pp. 1115—1119.

    Article  Google Scholar 

  23. Mastebroek, H.D., Balkema-Boomstra, A.G., and Gaj, M., Genetic analysis of powdery-mildew (Erysiphe graminis f. sp. hordei) derived from wild barley (Hordeum vulgare ssp. spontaneum), Plant Breed., 1995, vol. 114, pp. 121—125.

    Article  Google Scholar 

  24. Provan, J., Russell, J.R., Booth, A., and Powell, W., Polymorphic chloroplast simple sequence repeat primers for systematic and population studies in the genus Hordeum, Mol. Ecol., 1999, no. 8, pp. 505—511. https://doi.org/10.1046/j.1365-294X.1999.00545.x

  25. Harlan, J.R. and Zohary, D., Distribution of wild wheats and barley, Science, 1966, vol. 153, no. 3740, pp. 1074—1080. https://doi.org/10.1126/science.153.3740.1074

    Article  CAS  PubMed  Google Scholar 

  26. Willcox, G., Archeobotanical evidence for the beginnings of agriculture in Southwest Asia, The Origins of Agriculture and Crop Domestication (Proc. Harlan Symp.), Aleppo, 1997, pp. 25—38.

  27. Wendorf, F., Schild, R., El Hadidi, N., et al., Use of barley in the Egyptian late Paleolithic, Science, 1979, vol. 205, no. 4413, pp. 1341—1347. https://doi.org/10.1126/science.205.4413.1341

    Article  CAS  PubMed  Google Scholar 

  28. Zohary, D. and Hopf, M., Domestication of Plants in the Old World: The Origin and Spread of Cultivated Plants in West Asia, Europe, and Nile Valley, Oxford: Clarendon, 1988.

    Google Scholar 

  29. Pomortsev, A.A., Hordein polymorphism in Ethiopian barley, Russ. J. Genet., 2001, vol. 37, no. 10, pp. 1150—1160.

    Article  CAS  Google Scholar 

  30. Pomortsev, A.A., Kalabushkin, B.A., Ladogina, M., et al., Gene geography and specific features of the distribution of allelic variants in three hordein coding loci of spring barley on the territory of the former USSR, Genetika., 1994, vol. 30, no. 6, pp. 806—815.

    Google Scholar 

  31. Pomortsev, A.A., Kalabushkin, B.A., Blank, M.L., et al., Investigation of natural selection in artificial hybrid populations of spring barley, Russ. J. Genet., 1996, vol. 32, no. 11, pp. 1333—1341.

    CAS  Google Scholar 

  32. Pomortsev, A.A. and Lyalina, E.V., Allelic diversity of hordein-coding loci Hrd A and Hrd B in cultivated (Hordeum vulgare L.) and wild (H. spontaneum C. Koch) barley from Iran (as a part of the Fertile Crescent), Russ. J. Genet., 2016, vol. 52, no. 10, pp. 1034—1045.https://doi.org/10.1134/S1022795416100094

    Article  CAS  Google Scholar 

  33. Pomortsev, A.A., Boldyrev, S.V., and Lyalina, E.V., Allele diversity of hordein-coding loci Hrd A and Hrd B in cultivated (Hordeum vulgare L.) and wild (Hordeum spontaneum C. Koch) barley in Turkey (as part of the Fertile Crescent), Russ. J. Genet., 2017, vol. 53, no. 4, pp. 455—464. https://doi.org/10.1134/S1022795417030097

    Article  CAS  Google Scholar 

  34. Pomortsev, A.A., Boldyrev, S.V., and Lyalina, E.V., Allele diversity of hordein-coding loci Hrd A and Hrd B in cultivated (Hordeum vulgare L.) and wild (Hordeum spontaneum C. Koch) barley from Syria (as a part of the Fertile Crescent), Russ. J. Genet., 2018, vol. 54, no. 11, pp. 1275—1284. https://doi.org/10.1134/S0016675818110127

    Article  Google Scholar 

  35. Pomortsev, A.A., Rubanovich, A.V., Kovaleva, O.N., et al., Allelic diversity of Hrd A and Hrd B hordein-coding loci in wild (Hordeum spontaneum C. Koch) and cultivated (Hordeum vulgare L.) barley from Israel and Palestine, Russ. J. Genet., 2019, vol. 55, no. 11, pp. 1347—1359. https://doi.org/10.1134/S1022795419110097

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia

    A. A. Pomortsev, A. V. Rubanovich & E. V. Lialina

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  1. A. A. Pomortsev
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  2. A. V. Rubanovich
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Correspondence to A. A. Pomortsev.

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Translated by A. Lisenkova

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Pomortsev, A.A., Rubanovich, A.V. & Lialina, E.V. Structure and Formation Mechanisms of Polymorphism of Hordein-Coding Loci in Domesticated Barley (Hordeum vulgare L.). Russ J Genet 57, 548–560 (2021). https://doi.org/10.1134/S1022795421050082

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