Abstract
On the basis of the genetic analysis of F2 by F3 in the combination M13 × r “e” + o, the recombination frequencies between genetic factors of chromosome 6 (=6H) barley were estimated. The study included the following genes: r “e”(smooth awns “e”), sex 1 (shrunken endosperm xenia 1), o (orange lemma 1), gs 4 (glossy sheath 4), and Amy 1–7 (α-amylase 1, type 7). The Amy 1 locus was identified by separation of alpha-amylase by electrophoresis at pH 8.3. On the basis of the five-point test, the following order to location of the studied loci in chromosome 6 (=6H) was established: 6S–r “e” (=raw 5)–gs 4 (=gsh 4)–o (=rob 1)–cen–Sex 1–Amy 1–6L. The linkage estimation and literature analysis showed that, among the indicated genes, the Amy 1 and Sex 1 loci are located in the long arm of chromosome 6. The other genetic factors are associated with the short arm of this chromosome. The centromere is between the genetic factors of Sex 1 and o (=rob 1). In general, the data presented indicate the need to review the genetic linkage map of chromosome 6 (=6H) barley.
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REFERENCES
Schwimmer, S., The malt amylases, Brew. Dig., 1951, vol. 26, pp. 47–52.
Harris, G., The enzyme content and enzymatic transformation of malt, in Barley and Malt, Cook, A.H., Ed., New York, 1962, pp. 583–894.
Frydenberg, O. and Nielsen, G., Amylase isozymes in germinating barley seeds, Hereditas, 1966, vol. 54, no. 8, pp. 123–139.
Brown, A.H.D. and Jacobsen, J.V., Genetic basis and natural variation of α-amylase isozymes in barley, Genet. Res. Cambr., 1982, vol. 40, pp. 315–324. https://doi.org/10.1017/S0016672300019182
Netsvetaev, V.P., Location of the β-amylase coding locus (Bmy 1) on barley chromosome 4, Tsitol. Genet. (Kiev), 1993, vol. 27, no. 5, pp. 74–78.
Netsvetaev, V.P., Chromosomal location of loci Estl2 and Amy2 in barley, Barley Genet. Newslett., 1993, vol. 22, pp. 42–43.
Nielsen, G. and Frydenberg, O., Linkage between the loci Amy 1 (α-amylase), o (orange lemma) and x n (xanta seedling), Barley Genet. Newslett., 1974, vol. 4, рр. 53—54.
Søgaard, B. and von Wettstein-Knowless, P., Barley: genes and chromosomes, Carlberg Res. Commun., 1987, vol. 52, no. 2, pp. 123–196.
Tsuchiya, T., Linkage maps of barley, Barley Genet. Newsletter., 1984, vol. 14, pp. 81–84.
Franckowiak, J.D. and Lundqvist, U., Descriptions of barley genetic stocks tables 2 and 3, Barley Genet. Newsletter., 2018, vol. 48, pp. 13–189.
Rokitskii, P.F., Vvedenie v statisticheskuyu genetiku (Introduction to Statistical Genetics), Minsk: Vysheishaya shkola, 1974.
Allard, R.W., Formulas and tables to facilitate the calculation of recombination values in heredity, Hilgardia, 1956, vol. 24, no. 10, pp. 235–278.
Shahla, A., Shim, J.W., and Tsuchiya, T., Association of the gene o for orange lemma with the short arm of chromosome 6 (6S) in barley, Barley Genet. Newsletter., 1983, vol. 13, pp. 83–84.
Falk, D.E., Creation of a marked telo 6S trisomic for chromosome 6, Barley Genet. Newsletter., 1993, vol. 23, p. 32.
Falk, D.E., Coordinator’s report: chromosome 6, Barley Genet. Newsletter., 1993, vol. 23, p. 154.
Yoshimi, R. and Konishi, T., Linkage analysis of several isozyme loci in barley, Barley Genet. Newsletter., 1994, vol. 24, pp. 35–37.
Qi, X., Stam, P., and Lindhout, P., Comparison and integration of four barley genetic maps, Genome, 1996, vol. 39, pp. 379—394. https://doi.org/10.1139/g96-049
Langridge, P., Karakousis, A., Collins, N., et al., A consensus linkage map of barley, Mol. Breed., 1995, vol. 1, pp. 389—395.
Kozambi, D., The estimation of map distances from recombination values, Ann. Eugen., 1944, vol. 12, pp. 172–175.
Bailey, N.T.J., Introduction to the Mathematical Theory of Linkage, Oxford: Oxford Univ. Press, 1961.
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Netsvetaev, V.P. Linkage Analysis of Genes Controlling the Qualitative Characteristics of Barley Plants in Chromosome 6. Russ J Genet 57, 173–177 (2021). https://doi.org/10.1134/S1022795421020071
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DOI: https://doi.org/10.1134/S1022795421020071