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Variability of the mh44 Locus of Mitochondrial DNA in Siberian Spruce Populations

Abstract

The variability of the mitochondrial locus mh44 was studied in 24 natural populations of Siberian spruce (Picea obovata Ledeb.). Thirteen allelic variants differing in the number of repeats of the 32-nucleotide minisatellite motif were identified. The parameters of intra- and interpopulation diversity and the level of differentiation of the spruce populations included in the study were determined.

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REFERENCES

  1. Gugerli, F., Sperisen, C., Buchler, U., et al., Haplotype variation in a mitochondrial tandem repeat of Norway spruce (Picea abies) populations suggests a serious founder effect during postglacial recolonization of the western Alps, Mol. Ecol., 2001, vol. 10, no. 5, pp. 1255—1263. https://doi.org/10.1046/j.1365-294X.2001.01279.x

    CAS  Article  PubMed  Google Scholar 

  2. Ran, J.-H., Wei, X.-X., and Wang, X.-Q., Molecular phylogeny and biogeography of Picea (Pinaceae): implications for phylogeographical studies using cytoplasmic haplotypes, Mol. Phylogenet. Evol., 2006, vol. 41, no. 2, pp. 405—419. https://doi.org/10.1016/j.ympev.2006.05.039

    CAS  Article  PubMed  Google Scholar 

  3. Tollefsrud, M.M., Kissling, R., Gugerli, F., et al., Genetic consequences of glacial survival and postglacial colonization in Norway spruce: combined analysis of mitochondrial DNA and fossil pollen, Mol. Ecol., 2008, vol. 17, no. 18, pp. 4134—4150. https://doi.org/10.1111/j.1365_294X.2008.03893.x

    CAS  Article  PubMed  Google Scholar 

  4. Tollefsrud, M.M., Latałowa, M., van der Knaap, W.O., et al., Late quaternary history of North Eurasian Norway spruce (Picea abies) and Siberian spruce (Picea obovata) inferred from macrofossils, pollen and cytoplasmic DNA variation, J. Biogeogr., 2015, vol. 42, no. 8, pp. 1431—1442.https://doi.org/10.1111/jbi.12484

  5. Lockwood, J.D., Aleksic, J.M., Zou, J., et al., A new phylogeny for the genus Picea from plastid, mitochondrial, and nuclear sequences, Mol. Phylogenet. Evol., 2013, vol. 69, no. 3, pp. 717—727. https://doi.org/10.1016/j.ympev.2013.07.004

    Article  PubMed  Google Scholar 

  6. Semerikov, V.L., Semerikova, S.A., Putintseva, Yu.A., et al., Colonization history of Scots pine in Eastern Europe and North Asia based on mitochondrial DNA variation, Tree Genet. Genomes, 2018, vol. 14, no. 8. https://doi.org/10.1007/s11295-017-1222-0

  7. Mudrik, E.A., Polyakova, T.A., Shatokhina, A.V., et al., Spatial distribution of intron 2 of nad1 gene haplotypes in populations of Norway and Siberian spruce (Picea abiesP. obovata) species complex, Russ. J. Genet., 2015, vol. 51, no. 10, pp. 957—965. https://doi.org/10.1134/S1022795415100129

    CAS  Article  Google Scholar 

  8. Potokina, E.K., Kiseleva, A.A., Nikolaeva, M.A., et al., Analysis of polymorphism of organelle DNA to elucidate the phylogeography of Norway spruce in the East European Plain, Vavilovskii Zh. Genet. Sel., 2014, vol. 18, no. 4/1, pp. 818—830.

  9. Volkova, P., Shipunov, A., Borisova, P., et al., In search of hybridity: the case of Karelian spruces, Silva Fenn., 2014, vol. 48, no. 2, pp. 1—14. https://doi.org/10.14214/sf.1072

    Article  Google Scholar 

  10. Semerikov, V.L., Putintseva, Y.A., Oreshkova, N.V., et al., Development of new mitochondrial DNA markers in Scots pine (Pinus sylvestris L.) for population and phylogeographic studies, Russ. J. Genet., 2015, vol. 51, no. 12, pp. 1199—1203. https://doi.org/10.1134/S1022795415120108

    CAS  Article  Google Scholar 

  11. Ekart, A.K., Semerikov, V.L., Polezhaeva, M.A., et al., Development of mtDNA markers for population genetic studies of Siberian spruce (Picea obovata Ledeb.), Genetika populyatsii: progress i perspektivy (Genetics of Populations: Progress and Prospects) (Proc. Int. Sci. Conf., Moscow, April 17–21, 2017), Moscow: Vash Format, 2017, pp. 329—330.

  12. Semerikov, V.L., Semerikova, S.A., Putintseva, Yu.A., et al., Mitochondrial DNA in Siberian conifers indicates multiple postglacial colonization centers, Can. J. For. Res., 2019, vol. 49, pp. 875—883. https://doi.org/10.1139/cjfr-2018-0498

    Article  Google Scholar 

  13. Bastien, D., Favre, J.M., Collignon, A.M., et al., Characterization of a mosaic minisatellite locus in the mitochondrial DNA of Norway spruce (Picea abies (L.) Karst.), Theor. Appl. Genet., 2003, vol. 107, pp. 574—580. https://doi.org/10.1007/s00122-003-1284-2

    CAS  Article  PubMed  Google Scholar 

  14. Devey, M.E., Bell, J.C., Smith, D.N., et al., A genetic linkage map for Pinus radiata based on RFLP, RAPD and microsatellite markers, Theor. Appl. Genet., 1996, vol. 92, pp. 673—679. https://doi.org/10.1007/BF00226088

    CAS  Article  PubMed  Google Scholar 

  15. Peakall, R. and Smouse, P.E., GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update, Bioinformatics, 2012, no. 28, pp. 2537—2539. https://doi.org/10.1093/bioinformatics/bts460

  16. Nei, M., Molecular Evolutionary Genetics, New York: Columbia Univ. Press, 1987.

    Book  Google Scholar 

  17. Meirmans, P.G., Using the AMOVA framework to estimate a standardized genetic differentiation measure, Evolution, 2006, vol. 60, pp. 2399—2402. https://doi.org/10.1111/j.0014-3820.2006.tb01874.x

    Article  PubMed  Google Scholar 

  18. Semerikov, V.L., Semerikova, S.A., Polezhaeva, M.M., et al., Southern montane populations did not contribute to the recolonization of West Siberian Plain by Siberian larch (Larix sibirica): a range-wide analysis of cytoplasmic markers, Mol. Ecol., 2013, vol. 22, pp. 4958—4971. https://doi.org/10.1111/mec.12433

    CAS  Article  PubMed  Google Scholar 

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Funding

This study was carried out under basic research project no. 0356-2019-0024 with partial support from the Russian Foundation for Basic Research (project no. 13-04-00777a).

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Correspondence to A. K. Ekart, V. L. Semerikov or A. Ya. Larionova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Ekart, A.K., Semerikov, V.L., Larionova, A.Y. et al. Variability of the mh44 Locus of Mitochondrial DNA in Siberian Spruce Populations. Russ J Genet 56, 869–873 (2020). https://doi.org/10.1134/S1022795420070030

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Keywords:

  • Picea obovata
  • mitochondrial DNA
  • intra- and interpopulation genetic diversity
  • phylogeography