Skip to main content
SpringerLink
Log in

Development of 14 Microsatellite Markers in the Endangered Relict Plant Craigia yunnanensis (Tiliaceae)

  • SHORT COMMUNICATIONS
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Craigia yunnanensis (Tiliaceae) is an endangered relic tree in China. Its wild populations have been seriously damaged and fragmented due to severe human disturbance. In this study, we developed microsatellite markers in order to study the variation of the mating system of C. yunnanensis. We collected 19 067 214 unigenes sequences by shallow sequencing of the genome. A bioinformatics screening identified 15 235 unique and putative microsatellites, from which 590 novel microsatellite markers were developed. We designed 70 primer pairs and successfully amplified 14 of them in 30 individuals. The number of alleles per locus ranged from 1 to 12. The observed and expected heterozygosities ranged from 0.000 to 1.000 and 0.000 to 0.906, respectively. These microsatellite loci will enrich the genetic resources to develop functional studies and conservation strategies for this endangered relict species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Kvaček, Z., Manchester, S.R., Zetter, R., and Pingen, M., Fruits and seeds of Craigia bronnii (Malvaceae—Tilioideae) and associated flower buds from the late Miocene Inden formation, lower Rhine basin, Germany, Rev. Palaeobot. Palynol., 2002, vol. 119, nos. 3–4, pp. 311–324.

    Article  Google Scholar 

  2. Jin, J.H., Kodrul, T.M., Liao, W.B., and Wang, X., A new species of Craigia from the Eocene Changchang formation of Hainan Island, China, Rev. Palaeobot. Palynol., 2009, vol. 155, nos. 1–2, pp. 80–82.

    Article  Google Scholar 

  3. Qin, H.N., Yang, Y., and Dong, S.Y., et al. Threatened species list of China’s higher plants, Biodiversity Sci., 2017, vol. 25, no. 7, pp. 696–744.

    Article  Google Scholar 

  4. Gao, Z.R., Zhang, C.Q. and Milne, R.L., Size-class structure and variation in seed and seedling traits in relation to population size of an endangered species Craigia yunnanensis (Tiliaceae), Aust. J. Bot., 2010, vol. 58, no. 3, pp. 214–223.

    Article  Google Scholar 

  5. Yang, J., Gao, Z.R., Sun, W.B., and Zhang, C.Q., High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation, Plant Diversity, 2016, vol. 38, no. 5, pp. 221–226.

    Article  Google Scholar 

  6. Ashley, M.V. and Dow, B.D., The use of microsatellite analysis in population biology: background methods and potential applications, Exs, 1994, vol. 69, no. 69, pp. 185–201.

    CAS  PubMed  Google Scholar 

  7. Dawson, I.K., Waugh, R., Simons, A.J., and Powell, W., Simple sequence repeats provide a direct estimate of pollen-mediated gene dispersal in the tropical tree Gliricidia sepium,Mol. Ecol., 2003, vol. 6, no. 2, pp. 179–183.

    Article  Google Scholar 

  8. Yang, J., Zhao, L.L., Yang, J.B., and Sun, W.B., Genetic diversity and conservation evaluation of a critically endangered endemic maple, Acer yangbiense, analyzed using microsatellite markers, Biochem. System. Ecol., 2015, vol. 60, pp. 193–198.

    Article  CAS  Google Scholar 

  9. Liu, J. and Gao, L.M., Comparative analysis of three different methods of total DNA extraction used in Taxus,Guihaia, 2011, vol. 31, no. 2, pp. 244–238.

    CAS  Google Scholar 

  10. Meglécz, E., Costedoat, C., and Dubut, V., et al., QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects, Bioinformatics, 2010, vol. 26, no. 3, pp. 403–404.

    Article  Google Scholar 

  11. PeaKall, R. and Smouse, P.E., GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research: an update, Bioinformatics, 2012, vol. 28, pp. 2537–2539.

    Article  CAS  Google Scholar 

  12. Kalinowski, S.T., Taper, M.L., and Marshall, T.C., Revising how the computer program Cervus, accommodates genotyping error increases success in paternity assignment, Mol. Ecol., 2010, vol. 16, no. 5, pp. 1099–1106.

    Article  Google Scholar 

Download references

Funding

The study was conducted at the Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences. This work was supported by the National Natural Science Foundation of China (Grants no. 31600439), National Key R&D Program of China (2017YFC0505200) and Key Laboratory Construction of Yunnan Science and Technology Talents and Platform Program (Grant no. 2018DG004).

Author information

Authors and Affiliations

  1. Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Kunming Institute of Botany, Chinese Academy of Sciences, 650201, Kunming, China

    Y. L. Chen, J. Yang & W. B. Sun

  2. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, 650201, Kunming, China

    Y. L. Chen, J. Yang & W. B. Sun

  3. University of Chinese Academy of Sciences, 100049, Beijing, China

    Y. L. Chen

Authors
  1. Y. L. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. B. Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to J. Yang or W. B. Sun.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, Y.L., Yang, J. & Sun, W.B. Development of 14 Microsatellite Markers in the Endangered Relict Plant Craigia yunnanensis (Tiliaceae). Russ J Genet 56, 123–127 (2020). https://doi.org/10.1134/S1022795420010032

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795420010032

Keywords:

Search

Navigation