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Genetic Variability of Dolly Varden (Salvelinus malma), White-Spotted Char (S. leucomaenis), and Interspecific Hybrids from the Utkholok River (Northwestern Kamchatka)

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Abstract

The genetic variability of Dolly Varden, white-spotted char, and their interspecific hybrids for nine nuclear DNA microsatellite loci and the mtDNA CO1 gene was investigated. Significant genetic divergence between the two species of char for all microsatellite loci was shown (FST = 0.529; p < 0.001). Dolly Varden was characterized by significantly greater genetic variability compared to white-spotted char in terms of the average expected heterozygosity (HE) and allelic richness (AR). Hybrids had intermediate HE and AR values compared to their parents. Three haplotypes in the CO1 gene were revealed. All phenotypic white-spotted char individuals and the hybrids had one haplotype that was specific to S. leucomaenis. Specimens that were phenotypically identified as Dolly Varden had two haplotypes related to S. malma. Thus, the hybrids were produced by males of Dolly Varden and females of white-spotted char. The individuals with hybrid genotypes, except for one, were the first generation hybrids (F1). One char may be a descendant of a hybrid crossbreeding with one of the parental species or a second-generation hybrid F2, indicating that hybridization between the species studied is introgressive.

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ACKNOWLEDGMENTS

We are grateful to the participants of the Moscow State University–TCA (The Conservation Angler, Seattle, United States)–WSC (Wild Salmon Center, Portland, United States) expedition for their help in collecting the field material.

Funding

This work was carried out in the framework of the project of the Moscow State University “Noah’s Ark.”

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Authors and Affiliations

  1. Department of Ichthyology, Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    M. A. Gruzdeva, A. V. Semenova, K. V. Kuzishchin, E. V. Ponomareva & D. S. Pavlov

  2. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071, Moscow, Russia

    K. V. Kuzishchin & D. S. Pavlov

  3. Russian Federal Research Institute of Fisheries and Oceanography, 107140, Moscow, Russia

    A. A. Volkov

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  1. M. A. Gruzdeva
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  2. A. V. Semenova
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  3. K. V. Kuzishchin
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  4. E. V. Ponomareva
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  5. A. A. Volkov
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  6. D. S. Pavlov
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Correspondence to M. A. Gruzdeva.

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Conflicts of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals have been observed.

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Translated by K. Lazarev

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Gruzdeva, M.A., Semenova, A.V., Kuzishchin, K.V. et al. Genetic Variability of Dolly Varden (Salvelinus malma), White-Spotted Char (S. leucomaenis), and Interspecific Hybrids from the Utkholok River (Northwestern Kamchatka). Russ J Genet 56, 69–78 (2020). https://doi.org/10.1134/S1022795419090060

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