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Divergence of Oxytropis Species from the Section Verticillares (Fabaceae) of Steppe Flora of Baikal Siberia Based on Analysis of Chloroplast DNA

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Abstract

The analysis of nucleotide sequence polymorphism of chloroplast DNA psbA–trnH, trnL–trnF, and trnS–trnG intergenic spacers in the Oxytropis lanata, O. myriophylla, O. oxyphylla, O. selengensis, and O. stukovii (section Verticillares) of the steppe flora of Baikal Siberia demonstrated that the populations are characterized by a high haplotype (0.634–1.000) and relatively low (0.0003–0.0045) nucleotide diversity. Statistically significant values of genetic distances between the populations of the same species change from 0.19382 to 0.43449. Marker nucleotide substitutions and indels were detected in O. lanata, O. gracillima, O. interposita, O. pumila, and O. mongolica, indicating a significant divergence of their chloroplast genomes. A weak differentiation (ΦST = 0.137, P < 0.0001) between O. oxyphylla, O. selengensis, O. stukovii and O. bargusinensis and also low values of genetic distances (0.08939–0.32339) (corresponding to interpopulation distances), absence of differentiating molecular markers, formation of a single haplogroup in the median network, and presence of common haplotypes indicate that these species are a genetically homogeneous group probably formed as a result of relatively recent and rapid divergence from the common ancestor, as well as a high degree of interspecies hybridization.

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Funding

This study was supported by the Russian Foundation for Basic Research (project no. 16-04-01399) and by the Program of the Presidium of Russian Academy of Sciences “Far East” (project no. 18-4-011).

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Authors and Affiliations

  1. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    A. B. Kholina, M. M. Kozyrenko & E. V. Artyukova

  2. Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, 670047, Ulan-Ude, Russia

    D. V. Sandanov

Authors
  1. A. B. Kholina
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  2. M. M. Kozyrenko
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  3. E. V. Artyukova
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  4. D. V. Sandanov
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Correspondence to A. B. Kholina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Barkhash

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Kholina, A.B., Kozyrenko, M.M., Artyukova, E.V. et al. Divergence of Oxytropis Species from the Section Verticillares (Fabaceae) of Steppe Flora of Baikal Siberia Based on Analysis of Chloroplast DNA. Russ J Genet 55, 701–710 (2019). https://doi.org/10.1134/S102279541906005X

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