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Genotype of Wolbachia pipientis Endosymbiont Affects Octopamine Metabolism in Drosophila melanogaster Females

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Abstract

The effect of the genotype of the endosymbiont Wolbachia pipientis on the metabolism of octopamine (one of the main biogenic amines in insects) was studied in young Drosophila melanogaster females. The activity of tyrosine decarboxylase (key octopamine synthesis enzyme) and the activity of octopamine-dependent N-acetyltransferase (the enzyme of its degradation) were measured. It was demonstrated that the activity of both studied enzymes is increased under normal conditions in females infected with bacteria of the pathogenic wMelPop strain and decreased in those infected with bacteria of the wMelCS genotype, while it does not differ from those in uninfected flies in females infected with the wMel genotype. It was also found that the intensity of tyrosine decarboxylase response to heat stress is decreased in all females infected with Wolbachia. Thus, the effect of Wolbachia on octopamine metabolism was for the first time demonstrated in the Drosophila females, and it was shown that the nature of this effect is determined by the symbiont genotype.

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ACKNOWLEDGMENTS

We are grateful to Dr. Luis Teixeira (Instituto Gulbenkian de Ciência, Lisbon, Portugal) for providing the D. melanogaster line infected with the Wolbachia wMelPop strain.

Funding

This study was supported by the Russian Foundation for Basic Research (project no. 16-04-00060) and by the Budgetary Project no. 0324-2019-0016.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    N. V. Adonyeva, E. V. Burdina, R. A. Bykov, N. E. Gruntenko & I. Yu. Rauschenbach

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  1. N. V. Adonyeva
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  2. E. V. Burdina
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  3. R. A. Bykov
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  4. N. E. Gruntenko
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  5. I. Yu. Rauschenbach
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Correspondence to I. Yu. Rauschenbach.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by A. Barkhash

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Adonyeva, N.V., Burdina, E.V., Bykov, R.A. et al. Genotype of Wolbachia pipientis Endosymbiont Affects Octopamine Metabolism in Drosophila melanogaster Females. Russ J Genet 55, 653–655 (2019). https://doi.org/10.1134/S1022795419050028

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