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Genomic Profiling of the Response of Aspergillus oryzae to the Treatment with Bis(2-Pyridine-1-Oxide) Diselenide

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Abstract

We implemented genome-wide expression profiling to identify the mechanisms of toxicity of an organoselenium compound bis(2-pyridine-1-oxide) diselenide to the fungus А. oryzae RIB40. We uncovered changes in the expression levels in 72 genes. In particular, we observed a downregulation in the levels of several copper ion transmembrane transporter genes. In turn, we found a significant upregulation in the genes encoding oxidoreductases. The latter results are supported by biochemical experiments that revealed an increase in oxidoreductase activity in response to bis(2-pyridine-1-oxide) diselenide treatment. The results of a large-scale microarray analysis of the А. oryzae RIB 40 were confirmed by real-time quantitative PCR.

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ACKNOWLEDGMENTS

We are grateful to Prof. K. Gomi and the staff of the Bioindustrial Genomics Department of Tohoku University for providing the opportunities to conduct experiments and for consultations regarding these studies.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, Lobachevsky State University of Nizhny Novgorod, 603950, Nizhny Novgorod, Russia

    S. A. Zalepkina & V. F. Smirnov

  2. Department of Industrial Safety, Ecology, and Chemistry, Nizhny Novgorod State Technical University, 603950, Nizhny Novgorod, Russia

    A. V. Borisov & Zh. V. Matsulevich

Authors
  1. S. A. Zalepkina
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  2. V. F. Smirnov
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  3. A. V. Borisov
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  4. Zh. V. Matsulevich
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Correspondence to V. F. Smirnov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by I. Grishina

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Zalepkina, S.A., Smirnov, V.F., Borisov, A.V. et al. Genomic Profiling of the Response of Aspergillus oryzae to the Treatment with Bis(2-Pyridine-1-Oxide) Diselenide. Russ J Genet 55, 301–308 (2019). https://doi.org/10.1134/S1022795419030177

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