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Genomic Profiling of the Response of Aspergillus oryzae to the Treatment with Bis(2-Pyridine-1-Oxide) Diselenide

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We implemented genome-wide expression profiling to identify the mechanisms of toxicity of an organoselenium compound bis(2-pyridine-1-oxide) diselenide to the fungus А. oryzae RIB40. We uncovered changes in the expression levels in 72 genes. In particular, we observed a downregulation in the levels of several copper ion transmembrane transporter genes. In turn, we found a significant upregulation in the genes encoding oxidoreductases. The latter results are supported by biochemical experiments that revealed an increase in oxidoreductase activity in response to bis(2-pyridine-1-oxide) diselenide treatment. The results of a large-scale microarray analysis of the А. oryzae RIB 40 were confirmed by real-time quantitative PCR.

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We are grateful to Prof. K. Gomi and the staff of the Bioindustrial Genomics Department of Tohoku University for providing the opportunities to conduct experiments and for consultations regarding these studies.

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Correspondence to V. F. Smirnov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by I. Grishina

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Zalepkina, S.A., Smirnov, V.F., Borisov, A.V. et al. Genomic Profiling of the Response of Aspergillus oryzae to the Treatment with Bis(2-Pyridine-1-Oxide) Diselenide. Russ J Genet 55, 301–308 (2019).

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