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Genetic Differentiation of the Arctic Phylogenetic Group of Charr from Northeast Asia and North America

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Abstract

A comparative analysis of the mitochondrial DNA variability in Salvelinus taranetzi (and closely related taxa) from Asia and Salvelinus alpinus erythrinus from the Northwest Territories (NWT), North America, representing the Arctic phylogenetic group of charrs of the genus Salvelinus, has been carried out. The estimated divergence of the combined nucleotide sequences of the mtDNA CoI and Cytb genes and CR region within the discussed phylogroup varies in the range of 0.001–0.003 and does not exceed the intraspecific variability of S. taranetzi. A genealogical analysis confirms the phylogenetic proximity of S. a. erythrinus from NWT and S. taranetzi from Asia. By assuming a common origin of S. taranetzi and S. a. erythrinus, we have shown a significant differentiation between the geographical populations as a result of probable fragmentation of their common ancestor’s range. According to the hierarchical analysis of molecular variance (AMOVA), the intergroup component accounts for the major part (83% of variance) of the mtDNA variability (490 bp, CR) with a low genetic diversity within the groups (10%). The region of Anadyr–Kolyma Basin, which determines the heterogeneity of the phylogroups, stands out on a broad geographical scale.

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REFERENCES

  1. McPhail, J.D., A systematic study of the Salvelinus alpinus complex in Noth America, J. Fish. Res. Board Can., 1961, vol. 18, pp. 793—816.

    Article  Google Scholar 

  2. Behnke, R.J., A systematics review of the genus Salvelinus, in Charrs: Salmonid Fishes of the Genus Salvelinus, Hague: Junk, 1980, pp. 441—480.

  3. Behnke, R.J., Organizing the diversity of the Arctic charr complex, Biology of the Arctic Charr (Proc. Int. Symp. on Arctic Charr), Winnipeg: Univ. Manitoba Press, 1984, pp. 3—21.

  4. Savvaitova, K.A., Arkticheskie gol’tsy (struktura populyatsionnykh sistem, perspektivy khozyaistvennogo ispol’zovaniya) (Arctic Charrs: Structure of Population Systems and Perspectives of Commercial Use), Moscow: Agropromizdat, 1989.

  5. Wilson, C.C., Hebert, P.D.N., Reist, J.D., and Dempson, J.B., Phylogeography and postglacial dispersal of Arctic charr Salvelinus alpinus in North America, Mol. Evol., 1996, vol. 5, pp. 187—197.

    Google Scholar 

  6. Brunner, P.C., Douglas, M.R., Osinov, A., et al., Holarctic phylogeography of Arctic charr (Salvelinus alpinus L.) inferred from mitochondrial DNA sequences, Evolution, 2001, vol. 55, no. 3, pp. 573—586.

    Article  CAS  PubMed  Google Scholar 

  7. Osinov, A.G., Evolution relationships between the main taxa of the Salvelinus alpinus—S. malma complex: results of comparative analysis of allozyme data of different authors, J. Ichtyol., 2001, vol. 41, no. 2, pp. 192—208.

    Google Scholar 

  8. Radchenko, O.A., Variability of nucleotide sequences of mitochondrial DNA cytochrome b gene in chars of the genus Salvelinus, Russ. J. Genet., 2004, vol. 40, no. 3, pp. 244—254.

    Article  CAS  Google Scholar 

  9. Oleinik, A.G., Skurikhina, L.A., and Brykov, V.A., Divergence of the Salvelinus species mitochondrial DNA from northeastern Asia, Ecol. Freshwater Fish., 2007, vol. 16, no. 1, pp. 87—98.

    Article  Google Scholar 

  10. Taylor, E.B., Lowery, E., Liliestrale, A., et al., Genetic analysis of sympatric char populations in western Alaska: Arctic char (Salvelinus alpinus) and Dolly Varden (Salvelinus. malma) are not two sides of the same coin, J. Evol. Biol., 2008, vol. 21, pp. 1609—1625.

    Article  CAS  PubMed  Google Scholar 

  11. Alekseyev, S.S., Bajno, R., Gordeeva, N.V., et al., Phylogeography and sympatric differentiation of the Arctic charr Salvelinus alpinus (L.) complex in Siberia as revealed by mtDNA sequence analysis, J. Fish Biol., 2009, vol. 75, pp. 368—392.

    Article  CAS  PubMed  Google Scholar 

  12. Crête-Lafrenière, A., Weir, L.K., and Bernatchez, L., Framing the Salmonidae family phylogenetic portrait: a more complete picture from increased taxon sampling, PLoS One, 2012, vol. 7, no. 10. e46662.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Oleinik, A.G., Skurikhina, L.A. and Brykov, Vl.A., Phylogeny of charrs of the genus Salvelinus based on mitochondrial DNA data, Russ. J. Genet., 2015, vol. 51. no. 1, pp. 55—68.

    Article  CAS  Google Scholar 

  14. Moore, J.-S., Robert, B.R., Reist, J.D., and Taylor, E.B., Post-glacial recolonization of the north American Arctic by Arctic charr (Salvelinus alpinus): genetic evidence of multiple northern refugia and hybridization between glacial lineages, J. Biogeography, 2015, vol. 42, pp. 2089—2100.

    Article  Google Scholar 

  15. Glubokovskii, M.K. and Chereshnev, I.A., Controversial phylogeny issues of charr of the genus Salvelinus in Holarctic: 1. The study of anadromous charr of the East Siberian Sea, Vopr. Ikhtiol., 1981, vol. 21, no. 5, pp. 771—786.

    Google Scholar 

  16. Frolov, S.V., Izmenchivost’ i evolyutsiya kariotipov lososevykh ryb (Karyotype Variation and Evolution in Salmonids), Vladivostok: Dal’nauka, 2000.

  17. Osinov, A.G., Senchukova, A.L., Mugue, N.S., et al., Speciation and genetic divergence of three species of charr from ancient Lake El’gygytgyn (Chukotka) and their phylogenetic relationships with other representatives of the genus Salvelinus, Biol. J. Linn. Soc., 2015, vol. 116, no. 1, pp. 63—85.

    Article  Google Scholar 

  18. Esin, E.V., Bocharova, E.S., Mugue, N.S., and Markevich, G.N., Occurrence of sympatric charr groups, Salvelinus, Salmonidae, in the lakes of Kamchatka: a legacy of the last glaciations, J. Fish Biol., 2017, vol. 91, no. 2, pp. 628—644.

    Article  CAS  PubMed  Google Scholar 

  19. Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Lab. Press, 1989.

    Google Scholar 

  20. Uiblein, F., Jagsch, A., Honsig-Erlenburg, W., and Weiss, S., Status, habitat use, and vulnerability of the European grayling in Austrian waters, J. Fish. Biol., 2001, vol. 59, suppl. A, pp. 223—247.

  21. Ward, R.D., Zemlak, T.S., Innes, B.H., et al., DNA barcoding Australia’s fish species, Philos. Trans. R. Soc., B, 2005, vol. 360, pp. 1847—1857.

  22. Bandelt, H.-J., Foster, P., and Rohl, A., Median-joining networks for inferring intraspecific phylogenies, Mol. Biol. Evol., 1999, vol. 16, pp. 37—48.

    Article  CAS  PubMed  Google Scholar 

  23. Nei, M. and Kumar, S., Molecular Evolution and Phylogenetics, New York: Oxford Univ. Press, 2000.

    Google Scholar 

  24. Rozas, J., Ferrer-Mata, A., Sánchez-DelBarrio, et al., DnaSP 6: DNA Sequence Polymorphism Analysis of Large Datasets, Mol. Biol. Evol., 2017, vol. 34, pp. 3299—3302.

    Article  CAS  PubMed  Google Scholar 

  25. Doiron, S., Bernatchez, L., and Blier, P., A comparative genomics analysis of the potential adaptative value of Arctic charr mtDNA introgression in brook charr populations (Salvelinus fontinalis Mitchill), Mol. Evol. Biol., 2002, vol. 19, pp. 1902—1909.

    Article  CAS  Google Scholar 

  26. Cockerham, C.C., Analysis of gene frequencies, Genetics, 1973, vol. 74, pp. 679—700.

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Weir, B.S. and Cockerham, C.C., Estimating F statistics for the analysis of population structure, Evolution, 1984, vol. 38, pp. 1358—1370.

    CAS  PubMed  Google Scholar 

  28. Excoffier, L., Smouse, P.E., and Quattro, J.M., Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data, Genetics, 1992, vol. 131, pp. 479—491.

    CAS  PubMed  PubMed Central  Google Scholar 

  29. Excoffier, L. and Lischer, H.E.L., Arlequin Suite ver. 3.5: a new series of programs to perform population genetics analyses under Linux and Windows, Mol. Ecol. Resour., 2010, vol. 10, pp. 564—567. https://doi.org/10.1111/j.1755-0998.2010.02847.x

    Article  PubMed  Google Scholar 

  30. Oleinik, A.G., Skurikhina, L.A., and Kukhlevsky, A.D., Secondary contact between two divergent lineages of charrs of the genus Salvelinus in the northwest Pacific, Russ. J. Genet., 2017, vol. 53, no. 11, pp. 1221—1233.

    Article  CAS  Google Scholar 

  31. Ayers, S.D., A review of the species status of the Angayukaksurak charr (Salvelinus anaktuvukensis) of northern Alaska: perspectives from molecular and morphological data, MSc Thesis, University of Alaska, Fairbanks, 2010. http://www.arlis.org/docs/ vol1/B/608729840.pdf. Accessed March 21, 2018.

  32. Oleinik, A.G., Skurikhina, L.A., and Brykov, Vl.A., Differentiation of the Dolly Varden char (Salvelinus malma) and the Taranetz char (Salvelinus taranetzi) inferred from the PCR–RFLP analysis of mitochondrial DNA, Russ. J. Genet., 2004, vol. 40, no. 3, pp. 300—306. https://doi.org/10.1023/B:RUGE.0000021631.11738.25.

    Article  CAS  Google Scholar 

  33. Oleinik, A.G. and Skurikhina, L.A., Genetic divergence of sympatric charrs of the genus Salvelinus from Nachikinskoe Lake (Kamchatka), Russ. J. Genet., 2007, vol. 43, no. 8, pp. 910—917.

  34. Senchukova, A.L., Pavlov, S.D., Esin, E.V., et al., Charrs of the genus Salvelinus from Nachikinskoe Lake (Kamchatka Peninsula) and their position in the phylogenetic system of the S. alpinus—S. malma complex, J. Ichthyol., 2015, vol. 55, no. 1, pp. 97—104. https://doi.org/10.1134/S003294521501018X.

    Article  Google Scholar 

  35. Shedko, S.V., Miroshnichenko, I.L., and Nemkova, G.A., Phylogeny of salmonids (Salmoniformes: Salmonidae) and its molecular dating: analysis of mtDNA data, Russ. J. Genet., 2013, vol. 49, no. 6, pp. 623—637.

    Article  CAS  Google Scholar 

  36. Rodgers, T.W. and Mock, K.E., National Petroleum Reserve Alaska fish mitochondrial sequences, 2015 (unpublished).

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ACKNOWLEDGMENTS

This study is dedicated to the memory of the outstanding ichthyologist Igor Aleksandrovich Chereshnev. We are grateful to S.V. Frolov (National Scientific Center of Marine Biology (NSCMB), Far Eastern Branch, Russian Academy of Sciences (FEB RAS)), O.A. Radchenko (Institute of Biological Problems of the North, FEB RAS), and J.-S. Moore (Laval University, Institute of Integrative Biology and Systems, Quebec, Canada), who kindly provided the Salvelinus charr DNA preparations for the collection of the Laboratory of Genetics, NSCMB FEB RAS.

The study was supported by the Russian Foundation for Basic Research (project no. 15-04-01000).

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Authors and Affiliations

  1. Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia

    A. G. Oleinik, L. A. Skurikhina, A. D. Kukhlevsky & E. I. Bondar

  2. Department of Cell Biology and Genetics, Far Eastern Federal University, 690090, Vladivostok, Russia

    A. D. Kukhlevsky & E. I. Bondar

Authors
  1. A. G. Oleinik
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  2. L. A. Skurikhina
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  3. A. D. Kukhlevsky
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Correspondence to A. G. Oleinik.

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Translated by E. Shvetsov

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Oleinik, A.G., Skurikhina, L.A., Kukhlevsky, A.D. et al. Genetic Differentiation of the Arctic Phylogenetic Group of Charr from Northeast Asia and North America. Russ J Genet 55, 349–359 (2019). https://doi.org/10.1134/S1022795419030116

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