Russian Journal of Genetics

, Volume 54, Issue 5, pp 587–591 | Cite as

Isolation of Polymorphic RAPD-SSR Markers from Xinjiang Arctic Grayling (Thymallus arcticus grubei) and a Test of Cross-Species Amplification

Short Communications

Abstract

A total of 18 polymorphic microsatellite loci were isolated and characterized from RAPD products in the Xinjiang Arctic Grayling (Thymallus arcticus grubei). The number of alleles (Na) per locus varied from 2 to 10. Observed (Ho) and expected (He) heterozygosities ranged from 0.64 to 0.92, and from 0.63 to 0.88, respectively. Considerable differences were found among HBH, FH and FY populations in the number of alleles, effective number of alleles, number of genotypes at all of these loci. These new RAPD-SSR markers have provided a helpful tool for genetic analyses and resources conservation of T. arcticus grubei. Five additional fish species, Amur grayling (Thymallus grubii), Taimen (Hucho taimen), Sea perch (Lateolabrax japonicus), Lenok (Brachymystax lenok) and Red seam bream (Pagrosomus major) were assessed for cross-species amplification. Three of the five species showed at least one polymorphic locus. In addition, seven loci were found to be polymorphic in at least one species.

Keywords

Thymallus arcticus grubei microsatellite marker genetic diversity 

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  1. 1.College of Earth and Environment ScienceAnhui University of Science and TechnologyHuainanChina
  2. 2.College of Life Sciences and TechnologyXinjiang UniversityUrumqiChina
  3. 3.Linyi Academy of Agricultural SciencesLinyiChina

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