Abstract
Genotyping of TRPV1 and TRPA1 genes encoding thermoreceptors in the populations of the Altai-Sayan region and the Far East was conducted. The sample consisted of 15 populations comprising 1482 individuals. The analysis of TRPV1 rs222747 demonstrated that the frequency of M315I was closest to East Asian populations only in Nanais and Koryaks (56 and 64%, respectively). Siberian Tatars, Yakuts, and Evenks were closest to European populations. All populations of the Altai-Sayan region reported an intermediate position between the Caucasoids and the Eastern Mongoloids on the basis of the frequency of M315I. No deviations from the Hardy–Weinberg distribution were observed. The observed heterozygosity exceeded the expected one in eight populations. The analysis of TRPA1 rs13268757 revealed that Chukchi, Yukaghir, Koryak, Tuvinian, Southern Altaian, and Telengit populations were closest to the East Asian populations on the basis of the frequency of R3C substitution (3–7%). At the same time, populations of Siberian Tatars, Nanais, Evenks, Yakuts, Shorians, Khakases, and Kazakhs were intermediate between the Caucasoids (18–23%) and the Mongoloids from East Asia (3–7%) on the basis of the frequency of this polymorphism. A deviation from the Hardy–Weinberg distribution was detected only in the Yukaghir population. The observed heterozygosity was higher than the expected one in nine populations. A trans-association of TRPA1 and TRPV1 gene polymorphisms was carried out in 14 populations via regression analysis. A negative correlation of–0.545 was determined, the number of degrees of freedom (df) was 13, and the P-value was 0.048. The data obtained indicate that the analyzed polymorphisms are correlated, which confirms an earlier conclusion of the TRPA1-dependent inhibition of TRPV1 function. The results may evidence in the co-evolution of analyzed genes.
Similar content being viewed by others
References
Montell, C. and Rubin, G.M., Molecular characterization of the Drosophila trp locus: a putative integral membrane protein required for phototransduction, Neuron, 1989, vol. 2, pp. 1313–1323.
Diaz-Franulic, I., Poblete, H., Miño-Galaz, et al., Allosterism and structure in thermally activated receptor potential channels, Annu. Rev. Biophys., 2016, vol. 5, pp. 371–398. doi 10.1146/annurev-biophys-062215- 011034
Talavera, K., Yasumatsu, K., Voets, T., et al., Heat activation of TRPM5 underlies thermal sensitivity of sweet taste, Nature, 2005, vol. 438, pp. 1022–1025.
Nilius, B., Owsianik, G., and Voets, T. Transient receptor potential cation channels in disease, Physiol. Rev., 2007, vol. 87, pp. 165–217.
Caterina, M.J., Transient receptor potential ion channels as participants in thermosensation and thermoregulation, Am. J. Physiol., Regul. Integr. Comp. Physiol., 2007, vol. 292, pp. 64–76.
Vay, L., Gu, Ch., and McNaughton, P.A., The thermo-TRP ion channel family: properties and therapeutic implications, Br. J. Pharmacol., 2012, vol. 165, pp. 787–801.
Jordt, S.E., McKemy, D.D., and Julius, D. Lessons from peppers and peppermint: the molecular logic of thermosensation, Curr. Opin Neurobiol., 2003, vol. 13, pp. 487–492.
Saito, S. and Shingai, R., Evolution of thermo-TRP ion channel homologs in vertebrates, Physiol. Genomics, 2006, vol. 27, pp. 219–230.
Benninger, F., Freund, T.F., and Hajos, N. Control of excitatory synaptic transmission by capsaicin is unaltered in TRPV1 vanilloid receptor knockout mice, Neurochem. Int., 2008, vol. 52, pp. 89–94.
Liddle, R.A., The role of transient receptor potential vanilloid 1 (TRPV1) channels in pancreatitis, Biochim. Biophys. Acta, 2007, vol. 1772, pp. 869–878.
Story, G.M., Peier, A.M., Reeve, A.J., et al., ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures, Cell, 2003, vol. 112, pp. 819–829.
Laursen, W.J., Anderson, E.O., Hoffstaetter, L.J., et al., Species-specific temperature sensitivity of TRPA1, Temperature (Austin), 2015, vol. 11, pp. 214–226. doi 10.1080/23328940.2014.1000702
Cordero-Morales, J.F., Gracheva, E.O., and Julius, D. Cytoplasmic ankyrin repeats of transient receptor potential A1 (TRPA1) dictate sensitivity to thermal and chemical stimuli, Proc. Natl. Acad. Sci. U.S.A., 2011, vol. 108, pp. 1184–1191. doi 10.1073/pnas.1114124108
Moparthi, L., Survery, S., Kreir, M., et al., Human TRPA1 is intrinsically cold- and chemosensitive with and without its N-terminal ankyrin repeat domain, Proc. Natl. Acad. Sci. U.S.A., 2014, vol. 111, pp. 16901–16906. doi 10.1073/pnas.1412689111
Gaudet, R., A primer on ankyrin repeat function in TRP channels and beyond, Mol. Biosyst., 2008, vol. 4, pp. 372–379. doi 10.1039/b801481g
Derbenev, A.V. and Zsombok, A., Potential therapeutic value of TRPVI and TRPAI in diabetes mellitus and obesity, Semin. Immunopathol., 2016, vol. 38, pp. 397–406. doi 10.1007/s00281-015-0529-x
Salas, M.M., Hargreaves, K.M., and Akopian, A.N. TRPAI-mediated responses in trigeminal sensory neurons: interaction between TRPAI and TRPVI, Eur. J. Neurosci., 2009, vol. 29, pp. 1568–1578.
Fischer, M.J., Balasuriya, D., Jeggle, P., et al., Direct evidence for functional TRPVI/TRPAI heteromers, Pflugers Arch., 2014, vol. 466, pp. 2229–2241. doi 10.1007/s00424-014-1497-z
Di, D., Sanchez-Mazas, A., and Currat, M. Computer simulation of human leukocyte antigen genes supports two main routes of colonization by human populations in East Asia, BMC Evol. Biol., 2015, vol. 15, p. 240. doi 10.1186/s12862-015-0512-0
Ruhlen, M.A., Guide to the World’s Languages Classification, London: Edward Arnold, 1991.
Greenberg, J., Indo-European and Its Closest Relatives: The Eurasiatic Family: Grammar, Stanford, CA: Stanford Univ. Press, 2000.
Maniatis, T., Fritsch, E.E., and Sambrook, J., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Lab., 1982.
Slatkin, M., An exact test for neutrality based on the Ewens sampling distribution, Genet. Res., 1994, vol. 64, pp. 71–74.
Excoffier, L., Laval, G., and Schneider, S. Arlequin (version 3.0): an integrated software package for population genetics dataanalysis, Evol. Bioinform. Online, 2005, vol. 1, pp. 47–50.
http://www. Addinsoft.com.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © M.A. Gubina, P.S. Orlov, V.N. Babenko, A.P. Zabiyako, D.E. Ivanoschuk, M.I. Voevoda, 2018, published in Genetika, 2018, Vol. 54, No. 2, pp. 243–252.
Rights and permissions
About this article
Cite this article
Gubina, M.A., Orlov, P.S., Babenko, V.N. et al. Polymorphism of Human Thermoreceptor Genes TRPV1 and TRPA1 in Populations of the Altai-Sayan Region and the Far East. Russ J Genet 54, 235–243 (2018). https://doi.org/10.1134/S1022795418020084
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795418020084