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Polymorphism of the Avr2 gene of oomycete Phytophthora infestans (Mont.) de Bary in the population of Moscow region

  • Plant Genetics
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Abstract

The goal of the present study was to investigate the nucleotide sequence polymorphism of the Avr2 virulence gene in the population of P. infestans from Moscow region. The SSCP technique was applied for estimation of this polymorphism. As a result, the Avr2 gene was shown to have a high degree of polymorphism of the primary structure. In particular, seven variants of genotypes associated with the Avr2 gene polymorphism were identified. It was also found that the distribution of these genotypes among the samples of the studied population had a spatially dependent manner.

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References

  1. Vakhrusheva, O.A. and Nedospasov, S.A., System of innate immunity in plants, Mol. Biol. (Moscow), 2011, vol. 45, no. 1, pp. 16–25. https://doi.org/10.1134/S0026893311010146.

    Article  CAS  Google Scholar 

  2. Bent, A.F. and Mackey, D., Elicitors, effectors, and R genes: the new paradigm and a lifetime supply of questions, Annu. Rev. Phytopathol., 2007, vol. 45, pp. 399–436.

    Article  CAS  PubMed  Google Scholar 

  3. van der Biezen, E.A. and Jones, J.D., Plant diseaseresistance proteins and the gene-for-gene concept, Trends Biochem. Sci., 1998, vol. 23, pp. 454–456.

    Article  PubMed  Google Scholar 

  4. Armstrong, M.R., Whisson, S.C., Pritchard, L., et al., An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm, Proc. Natl. Acad. Sci. U.S.A., 2005, vol. 102, pp. 7766–7771.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. van Poppel, P.M.J.A., Guo, J., Van de Vondervoort, P.J.I., et al., The Phytophthora infestans avirulence gene Avr4 encodes an RXLR-dEER effector, Mol. Plant—Microbe Interact., 2008, vol. 21, pp. 1460–1470.

    Article  PubMed  Google Scholar 

  6. Champouret, N., Bouwmeester, K., Rietman, H., et al., Phytophthora infestans isolates lacking class I ipiO variants are virulent on Rpi-blb1 potato, Mol. Plant—Microbe Interact., 2009, vol. 22, pp. 1535–1545.

    Article  CAS  PubMed  Google Scholar 

  7. Gilroy, E.M., Breen, S., Whisson, S.C., et al., Presence/absence, differential expression and sequence polymorphisms between PiAVR2 and PiAVR2-like in Phytophthora infestans determine virulence on R2 plants, New Phytol., 2011, vol. 191, pp. 763–776.

    Article  CAS  PubMed  Google Scholar 

  8. Kamoun, S., Molecular genetics of pathogenic oomycetes, Eukaryotic Cell, 2003, vol. 2, no. 2, pp. 191–199.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Chamnanpunt, J., Shan, W.X., and Tyler, B.M., High frequency mitotic gene conversion in genetic hybrids of the oomycete Phytophthora sojae, Proc. Natl. Acad. Sci. U.S.A., 2001, vol. 98, pp. 14530–14535.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Judelson, H.S., Sequence variation and genomic amplification of a family of Gypsy-like elements in the oomycete genus Phytophthora, Mol. Biol. Evol., 2002, vol. 19, pp. 1313–1322.

    Article  CAS  PubMed  Google Scholar 

  11. Ristaino, J.B., Groves, C.T., and Parra, G.R., PCR amplification of the Irish potato famine pathogen from historic specimens, Nature, 2001, vol. 411, pp. 695–697.

    Article  CAS  PubMed  Google Scholar 

  12. Nahiyan, A.S.M., Boyer, L.R., Jeffries, P., and Matsubara, Y., PCR-SSCP analysis of Fusarium diversity in asparagus decline in Japan, Eur. J. Plant Pathol., 2011, vol. 130, pp. 197–203.

    Article  Google Scholar 

  13. Jiang, R.H., Weide, R., van de Vondervoort, P.J., and Govers, F., Amplification generates modular diversity at an avirulence locus in the pathogen Phytophthora, Genome Res., 2006, vol. 16, pp. 827–840.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Qutob, D., Tedman-Jones, J., Dong, S., et al., Copy number variation and transcriptional polymorphisms of Phytopthora sojae RXLR effector genes Avr1a and Avr3a, PLoS One, 2009, vol. 4. e5066

    Article  PubMed  PubMed Central  Google Scholar 

  15. Dong, S., Qutob, D., Tedman-Jones, J., et al., The Phytophthora sojae avirulence locus Avr3c encodes a multi-copy RXLR effector with sequence polymorphisms among pathogen strains, PLoS One, 2009, vol. 4. e5556

    Article  PubMed  PubMed Central  Google Scholar 

  16. Elansky, S., Smirnov, A., Dyakov, Y., et al., Genotypic analysis of Russian isolates of Phytophthora infestans from the Moscow region, Siberia and Far East, J. Phytopathol., 2001, vol. 149, pp. 605–611.

    Article  Google Scholar 

  17. Elansky, S.N., Smirnov, A.N., Dolgova, A.V., and Dyakov, Yu.T., The Phytophthora infestans populations in Moscow region: 1. Reproduction systems, Mycol. Phytopathol., 1999, vol. 33, no. 5, pp. 346–352.

    Google Scholar 

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Correspondence to V. V. Martynov.

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Original Russian Text © V.K. Chizhik, V.V. Martynov, 2017, published in Genetika, 2017, Vol. 53, No. 12, pp. 1411–1418.

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Chizhik, V.K., Martynov, V.V. Polymorphism of the Avr2 gene of oomycete Phytophthora infestans (Mont.) de Bary in the population of Moscow region. Russ J Genet 53, 1328–1334 (2017). https://doi.org/10.1134/S1022795417120031

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  • DOI: https://doi.org/10.1134/S1022795417120031

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