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Genotyping of patients with phenylketonuria from different regions of Russia for determining BH4 responsiveness

  • Human Genetics
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Abstract

To date, the efficacy of the phenylalanine hydroxylase (PAH) cofactor is proved for the treatment of both BH4-dependent hyperphenylalaninemia and phenylketonuria patients with mutations in the PAH gene. Since the patient’s response depends on the presence of residual PAH enzyme activity, it is advisable to search for mutations in the PAH gene to identify the potential responders and nonresponders to therapy. Four hundred thirty-five phenylketonuria patients from 13 regions of the Russian Federation were genotyped in order to identify responders and nonresponders to tetrahydrobiopterin (BH4) therapy. According to the results of this study, the number of probable nonresponders to the BH4 treatment exceeds 50% owing to a higher overall allelic frequency of “severe” PAH gene mutations. Responder patients with two “mild” mutations in the PAH gene were identified (1.6%).

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Authors and Affiliations

  1. Research Centre for Medical Genetics, Moscow, 115478, Russia

    P. Gundorova, A. A. Stepanova, R. A. Zinchenko, S. I. Kutsev & A. V. Polyakov

  2. Scientific Center of Children’s Health, Ministry of Health of the Russian Federation, Moscow, 119296, Russia

    T. V. Bushueva

  3. Medical and Genetic Counseling, Orenburg Regional Clinical Hospital No. 2, Orenburg, 460035, Russia

    E. Yu. Belyashova

  4. Pirogov Russian National Research Medical University, Moscow, 117997, Russia

    R. A. Zinchenko & S. I. Kutsev

  5. Rostov State Medical University, Rostov-on-Don, 344022, Russia

    S. S. Amelina

Authors
  1. P. Gundorova
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  2. A. A. Stepanova
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  3. T. V. Bushueva
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  4. E. Yu. Belyashova
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  5. R. A. Zinchenko
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  6. S. S. Amelina
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  7. S. I. Kutsev
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  8. A. V. Polyakov
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Correspondence to P. Gundorova.

Additional information

Original Russian Text © P. Gundorova, A.A. Stepanova, T.V. Bushueva, E.Yu. Belyashova, R.A. Zinchenko, S.S. Amelina, S.I. Kutsev, A.V. Polyakov, 2017, published in Genetika, 2017, Vol. 53, No. 6, pp. 732–739.

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Gundorova, P., Stepanova, A.A., Bushueva, T.V. et al. Genotyping of patients with phenylketonuria from different regions of Russia for determining BH4 responsiveness. Russ J Genet 53, 712–718 (2017). https://doi.org/10.1134/S1022795417060060

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  • DOI: https://doi.org/10.1134/S1022795417060060

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