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Comparative Estimate of the sheep breed gene pools using ISSR-analysis

  • Animal Genetics
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Abstract

Using the ISSR-PCR technique, the genetic structure of nine sheep breeds (Ovis aries) bred on the territories of Russia and Mongolia was examined. Species-specific and breed-specific DNA fragments were identified. For the first time, data on the genetic diversity of Telengit and Buubey sheep breeds were obtained. The main parameters of the genetic diversity and the breed structure were assessed, and the phylogenetic relationships and genetic distances between the studied breeds were determined. Using the method of hierarchical frequency averaging, the prototypal sheep gene pool was reconstructed. The three-tiered analysis of diversity based on the ISSR fingerprinting data showed that 15.8% of variability was found between the breeds, 31.4% of variability was found between the populations within the breeds, and the diversity among the individuals within the populations constituted 52.8%.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    L. V. Nesteruk, A. N. Evsyukov, G. R. Svishcheva & Yu. A. Stolpovsky

  2. OOO AgriVolga, Uglich, 152615, Russia

    N. N. Makarova

  3. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    G. R. Svishcheva

  4. Shuluuma IAPC, Kizhinga, 671450, Buryatia, Russia

    B. B. Lhasaranov

Authors
  1. L. V. Nesteruk
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  2. N. N. Makarova
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  3. A. N. Evsyukov
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  4. G. R. Svishcheva
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  5. B. B. Lhasaranov
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  6. Yu. A. Stolpovsky
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Correspondence to L. V. Nesteruk.

Additional information

Original Russian Text © L.V. Nesteruk, N.N. Makarova, A.N. Evsyukov, G.R. Svishcheva, B.B. Lhasaranov, Yu.A. Stolpovsky, 2016, published in Genetika, 2016, Vol. 52, No. 3, pp. 346–356.

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Nesteruk, L.V., Makarova, N.N., Evsyukov, A.N. et al. Comparative Estimate of the sheep breed gene pools using ISSR-analysis. Russ J Genet 52, 304–313 (2016). https://doi.org/10.1134/S102279541603011X

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  • DOI: https://doi.org/10.1134/S102279541603011X

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