Russian Journal of Genetics

, Volume 51, Issue 10, pp 1036–1039 | Cite as

Synaptonemal complex of two fish species of the genus Nothobranchius (Cyprinodontidae)

  • L. D. SafronovaEmail author
  • E. J. Krysanov
Short Communications


The karyotype of males of two species of the genus Nothobranchius with the lowest diploid numbers was investigated: Nothobranchius rachovi (2N = 16) and Nothobranchius krysanovi (2N = 18). For the first time, whole mounts of spread syneptonemal complexes (SC) of these species were obtained and investigated. When the SC was painted with silver nitrate and immunostained with rabbit antibodies against the SCP3 protein, eight and nine homomorphic SCs were detected in the nuclei of class I spermatocytes of N. krysanovi and N. rachovi, respectively. The sex chromosomes were not identified.


meiosis synaptonemal complex genus Nothobranchius 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Wildekamp, R., A World of Killies, Am. Killifish Assoc., 2004.Google Scholar
  2. 2.
    Shidlovsky, K.M., Watters, B.R., and Wildekamp, R.H., Notes on annual killifish species Nothobranchius rachovi (Cyprinodontiformes; Nothobranchidae) with the description of two new species, Zootaxa, 2010, vol. 2724, pp. 37–57.Google Scholar
  3. 3.
    Ewulonu, U., Haas, R., and Turner, B., A multiple sex chromosome system in the annual killifish, Nothobranchius guentheri, Copeia, 1985, vol. 2, pp. 503–508.CrossRefGoogle Scholar
  4. 4.
    Dresser, M. and Moses, M., Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griscus): 4. Light and electron microscopy of synapsis and nucleolar development by silver staining, Chromosoma, 1980, vol. 76, pp. 1–22.CrossRefPubMedGoogle Scholar
  5. 5.
    Bogdanov, Yu.F. and Kolomiets, O.L., Karyotyping based on synaptonemal complexes and the use of this method in cytogenetics, Genetika (Moscow), 1985, vol. 21, no. 5, pp. 793–802.Google Scholar
  6. 6.
    Safronova, L.D., Kolomiets, O.L., Bogdanov, Yu.F., et al., Association between synaptonemal complexes of sex and autosomal bivalents in male tx/ty mice as a possible cause of their sterility, Genetika (Moscow), 1988, vol. 24, no. 7, pp. 1187–1198.Google Scholar
  7. 7.
    Wallace, B. and Wallace, H., Synaptonemal complex karyotype of zebrafish, Heredity, 2003, vol. 90, pp. 136–140.CrossRefPubMedGoogle Scholar
  8. 8.
    Traut, W. and Winking, H., Meiotic chromosomes and stages of sex chromosome evolution in fish: zebrafish, platyfish and guppy, Chromosome Res., 2001, vol. 9, no. 8, pp. 658–672.CrossRefGoogle Scholar
  9. 9.
    Cunado, N., Terrones, J., Sanchez, L., et al., Synaptonemal complex analysis in spermatocytes and oocytes of turbot Scophtalmus maximus (Pisces, Scophthalmidae), Genome, 2001, vol. 7, no. 4, pp. 1143–1447.CrossRefGoogle Scholar
  10. 10.
    Foresti, F., Oliveira, C., Galleti, P., et al., Synaptonemal complex analysis in spermatocytes of tilapia Dreochromis niloticus (Pisces, Cichlidae), Genome, 1993, vol. 36, pp. 1124–1128.CrossRefPubMedGoogle Scholar
  11. 11.
    Kligerman, A. and Bloom, S., Rapid chromosome preparations from solid tissue of fishes, J. Fish. Res., 1977, vol. 34, pp. 266–269.CrossRefGoogle Scholar
  12. 12.
    Kolomiets, O.L., Matveevsky, S.N., and Bakloushinskaya, I.Y., Sexual dimorphism in prophase I of meiosis in the northern mole vole (Ellobius talpinus Pallas, 1770) with isomorphic (XX) chromosomes in males and females, Comp. Cytogenet., 2010, vol. 4, no. 1, pp. 55–66.CrossRefGoogle Scholar
  13. 13.
    Fraune, J., Alsheimer, M., Volff, J.N., et al., Hydra meiosis reveals unexpected conservation of structural synaptonemal complex proteins across metazoans, Proc. Natl. Acad. Sci. U.S.A., 2012, vol. 109, no. 41, pp. 16588–16593.PubMedCentralCrossRefPubMedGoogle Scholar
  14. 14.
    Grishaeva, T.M. and Bogdanov, Y.F., Conservation and variability of synaptonemal complex proteins in phylogenesis of eukaryotes, Int. J. Evol. Biol., 2014, vol. 2014, Article ID 85623. doi l0.1155/2014/856230Google Scholar
  15. 15.
    White, M.J., Animal Cytology and Evolution, Cambridge, 1973.Google Scholar
  16. 16.
    Tripath, N., Hoffmann, M., Weigel, D., and Dreyer, C., Linkage analysis reveals the independent origin of poeciliid sex chromosomes and a case of atypical sex inheritance in the guppy (Poecilia reticulata), Genetics, 2009, vol. 182, no. 1, pp. 365–374.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2015

Authors and Affiliations

  1. 1.Severtsov Institute of Ecology and EvolutionRussian Academy of SciencesMoscowRussia

Personalised recommendations