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Spatial distribution of intron 2 of nad1 gene haplotypes in populations of Norway and Siberian spruce (Picea abies–P. obovata) species complex

  • Plant Genetics
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Abstract

The length and sequence variations among intron 2 haplotypes of the mitochondrial nad1 gene have been studied in the Norway and Siberian spruce (Picea abies (L.) H. Karst.–P. obovata Ledeb.) species complex. Twenty-two native populations and 15 provenances were analyzed. The distribution of the northern European haplogroup (haplotypes 721, 755, 789, 823, 857, 891, and 925) is delimited in the east by the Ural region inclusively. Haplotype 712 is widespread in populations of Siberia and in the Far East and in northeastern Russia. A novel variant of the Siberian haplogroup (780) containing three copies of the first minisatellite motif (34 bp) was found for the first time. The absence of an admixture of the northern European and Siberian haplotypes in the zone of spruce species introgression previously marked by morphological traits and nuclear allozyme loci was demonstrated. This may be evidence of the existence of a sharper geographic boundary between the two haplogroups, as compared to a boundary based on phenotypic and allozyme data. A high proportion of the interpopulation component of variation (65%) estimated by AMOVA indicates a substantial genetic subdivision of European and Siberian populations of the Palearctic spruce complex by mtDNA, which can be putatively explained by natural barriers to gene flow with seeds related, for instance, to the woodless regions of the western Siberian Plain in the Pleistocene and perhaps to the floodplains of large rivers.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    E. A. Mudrik, T. A. Polyakova, A. V. Shatokhina, G. N. Bondarenko & D. V. Politov

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  1. E. A. Mudrik
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Original Russian Text © E.A. Mudrik, T.A. Polyakova, A.V. Shatokhina, G.N. Bondarenko, D.V. Politov, 2015, published in Genetika, 2015, Vol. 51, No. 10, pp. 1117–1125.

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Mudrik, E.A., Polyakova, T.A., Shatokhina, A.V. et al. Spatial distribution of intron 2 of nad1 gene haplotypes in populations of Norway and Siberian spruce (Picea abies–P. obovata) species complex. Russ J Genet 51, 957–965 (2015). https://doi.org/10.1134/S1022795415100129

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