Skip to main content
SpringerLink
Log in

Molecular analysis of new sources of resistance to Pseudoperonospora cubensis (Berk. et Curt.) Rostovzev in cucumber

  • Plant Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Downy mildew of cucumber (Cucumis sativus L.), caused by Pseudoperonospora cubensis (Berk. et Curt.) Rostovzev, is one of the most important foliar diseases of cucurbit crops. Two parental lines resistant PI 197085, susceptible PI 175695 and their F2 generation were used in our study. Inheritance of resistance to Pseudoperonospora cubensis in PI 197085 was quantitative. JoiMap 4.1 and MapQTL 6.0 software was used for a linkage groups construction and QTL mapping. Three QTL were detected: DM1, DM2, DM3. The loci were mapped on chromosome 5 of cucumber genome. Molecular analysis confirmed results of classical quantitative genetics indicating that resistance to Pseudoperonospora cubensis in PI 197085 is polygenic trait.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lebeda, A. and Urban, J., Distribution, harmfulness and pathogenic variability of cucumber downy mildew in the Czech Republic, Acta Fytotech. Zootech., 2004, vol. 7, pp. 170–173.

    Google Scholar 

  2. Lebeda, A. and Cohen, Y., Cucurbit downy mildew (Pseudoperonospora cubensis) biology, ecology, epidemiology, host–pathogen interaction and control, Eur. J. Plant Pathol., 2011, vol. 129, pp. 157–192.

    Article  Google Scholar 

  3. Olczak-Woltman, H., Marcinkowska, J., and Niemirowicz-Szczyt, K., The genetic basis of resistance to downy mildew in Cucumis spp.—latest developments and prospects, J. Appl. Genet., 2011, vol. 52, pp. 249–255.

    Article  PubMed Central  PubMed  Google Scholar 

  4. Cohen, Y. and Rotem, J., Dispersal and viability of sporangia of Pseudoperonospora cubensis, Trans. Br. Mycol. Soc., 1971, vol. 57, pp. 67–74.

    Article  Google Scholar 

  5. Kanetis, L., Holmes, G.J., and Ojiambo, P.S., Survival of Pseudoperonospora cubensis sporangia exposed to solar radiation, Plant Pathol., 2010, vol. 59, pp. 313–323.

    Article  Google Scholar 

  6. Lange, L., Eden, U., and Olson, L.W., Zoosporogenesis in Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew, Nord. J. Bot., 1989, vol. 8, pp. 497–504.

    Article  Google Scholar 

  7. Lebeda, A., Resistance in muskmelons to Czechoslovak isolates of Pseudoperonospora cubensis from cucumbers, Sci. Hortic., 1991, vol. 45, nos. 3–4, pp. 225–260.

    Google Scholar 

  8. Rondoman’ski, W., Downy mildew on cucumber a serious problem in Poland, Abstracts of Papers 5th International Congress of Plant Pathology, Kyoto, 1988, poster pp. VIII: 2–48.

    Google Scholar 

  9. Epinat, C., and Pitrat, M., Inheritance of resistance to downy mildew (Pseudoperonospora cubensis) in muskmelon (Cucumis melo L.): analysis of a 8 × 8 diallel table, Agronomice, 1994, vol. 14, pp. 239–248.

    Article  Google Scholar 

  10. van Vielt, G.J.A. and Meysing, W.D., Inheritance of resistance to Pseudoperonospora cubensis Rost. in cucumber (Cucumis sativus L.), Euphytica, 1974, vol. 23, pp. 251–255.

    Article  Google Scholar 

  11. Doruchowski, R.W., and kowska-Ryk, E., Inheritance of resistance to downy mildew (Pseudoperonospora cubensis Berk. and Curt.), in Cucumis sativus, in Proceedings of 5th Eucarpia Cucurbitaceae Symposium, Doruchowski, R.W., Kozik, E., and Niemirowicz-Szczyt, K., Eds., Skierniewice-Warszawa, 1992, pp. 133–135.

    Google Scholar 

  12. Jenkins, J.M., Studies on the inheritance of downy mildew resistance, J. Hered., 1946, vol. 37, pp. 267–276.

    PubMed  Google Scholar 

  13. Kozik, E.U., Klosinska, U., Call, A.D., et al., Heritability and genetic variance estimates for resistance to downy mildew in cucumber accession ames 2354, Crop Sci., 2013, vol. 53, no. 1, pp. 177–182.

    Article  Google Scholar 

  14. Zhang, S.P., Liu, M.M., and Miao, H., Chromosomal mapping and QTL analysis of resistance to downy mildew in Cucumis sativus, Plant Dis., 2013, vol. 97, pp. 245–251.

    Article  CAS  Google Scholar 

  15. Pang, X., Zhou, X., Wan, H., and Chen, J., QTL mapping of downy mildew resistance in an introgression line derived from interspecific hybridization between cucumber and Cucumis hystrix, J. Phytopathol., 2013, vol. 161, pp. 536–543.

    Article  Google Scholar 

  16. Klosinska, U., Kozik, E.U., Call, A., and Wehner, T.C., New sources of resistance to downy mildew in cucumber, in Cucurbitaceae Proc., Thies, J.A., Kousik, S., and Levi, A., Eds., Charleston, SC: U.S. Vegetable Laboratory, 2010, pp. 135–148.

    Google Scholar 

  17. Call, A.D., Criswell, A.D., Wehner, T.C., et al., Screening cucumber for resistance to downy mildew caused by Pseudoperonospora cubensis (Berk. & Curt.) Rostov., Crop Sci., 2012, vol. 52, pp. 577–592.

    CAS  Google Scholar 

  18. Jenkins, S.F., Jr. and Wehner, T.C., A system for the measurement of foliar diseases in cucumbers, Cucurbit Genet. Coop. Rpt., 1983, vol. 6, pp. 10–12.

    Google Scholar 

  19. Horejsi, T., Staub, J.E., and Thomas, C., Linkage of random amplified polymorphic DNA markers to downy mildew resistance in cucumber (Cucumis sativus L.), Euphytica, 2000, vol. 115, pp. 105–113.

    Article  CAS  Google Scholar 

  20. Ren, Y., Zhang, Z., Liu, J., et al., An integrated genomic and cytogenetic map of the cucumber genome, PLoS One, 2009, vol. 4, no. 6. e5795

    Article  PubMed Central  PubMed  Google Scholar 

  21. Bai, Z., Yuan, X., Cai, R., et al., QTL analysis of downy mildew resistance in cucumber, Prog. Nat. Sci., 2008, vol. 18, pp. 706–710.

    CAS  Google Scholar 

  22. Wang, Y., VandenLangenberg, K., Wehner, T.C., et al., QTLS for downy mildew resistance and their association with LRR-RLK resistance gene analogs in cucumber, in Biotic Stresses—Pathology and Entomology, Proc. Cucurbitaceae, Bay Harbor, Michigan, 2014, pp. 17–20.

    Google Scholar 

  23. Liu, D., Feng, G., and Sheng, H., Differential analysis of gene expression profiling in different cucumber cultivars inoculated with Pseudoperonospora cubensis, in Biotic Stresses—Pathology and Entomology, Proc. Cucurbitaceae, Bay Harbor, Michigan, 2014, pp. 4–6.

    Google Scholar 

  24. Huang, S., Li, R., Zhang, Z., et al., The genome of the cucumber, Cucumis sativus L., Nat. Genet., 2009, vol. 41, pp. 1275–1281.

    Article  CAS  PubMed  Google Scholar 

  25. Zhang, S., Liu, M., Miao, H., et al., QTL mapping of resistance genes to powdery mildew in cucumber (Cucumis sativus L.), Sci. Agric. Sin., 2011, vol. 44, pp. 3584–3593.

    Google Scholar 

  26. Xiaoming, H., Yuhong, L., Yandell, B.S., et al., QTL mapping of powdery mildew resistance in WI 2757 cucumber (Cucumis sativus L.), Theor. Appl. Genet., 2013, vol. 126, pp. 2149–2161.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute of Horticulture, 96-100, Skierniewice, Poland

    W. Szczechura, M. Staniaszek, U. Klosinska & E. U. Kozik

Authors
  1. W. Szczechura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Staniaszek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. U. Klosinska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. U. Kozik
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to W. Szczechura.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Szczechura, W., Staniaszek, M., Klosinska, U. et al. Molecular analysis of new sources of resistance to Pseudoperonospora cubensis (Berk. et Curt.) Rostovzev in cucumber. Russ J Genet 51, 974–979 (2015). https://doi.org/10.1134/S1022795415090112

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795415090112

Keywords

Search

Navigation