Skip to main content
SpringerLink
Log in

Knockdown of InR gene in ventral nephrocytes promotes resistance to toxic stress in Drosophila melanogaster females

  • Short Communications
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Hemolymph filtration in insects is performed by nephrocytes, additional cells of the circulatory system that are not connected to Malpighian vessels. Drosophila has two types of nephrocytes: the ventral (“garland”), which are situated around the connection site of the esophagus and proventriculus, and the pericardial, which are localized around the heart. In this study, we examined the role of the of insulin-like receptor (InR) gene in regulation of the function of ventral nephrocytes (VNC) in D. melanogaster females. Immunofluorescent analysis of female VNC with anti-InR antibodies revealed for the first time that the InR gene is expressed in VNC cells. To determine whether a change in the level of InR expression has an effect on VNC function in Drosophila females, we implemented an antisense suppressor of the InR gene, together with a driver that is expressed specifically in VNC. VNC function was evaluated by survival of the females exposed to toxic stress (treatment with AgNO3). This study has shown for the first time that suppression of InR expression in VNC leads to a rise in the survival of flies under conditions of toxic stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Dow, J.A. and Romero, M.F., Drosophila provides rapid modeling of renal development, function, and disease, Am. J. Physiol. Renal Physiol., 2010, vol. 299, pp. F1237–F1244.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Weavers, H., Prieto-Sanchez, S., Grawe, F., et al., The insect nephrocyte is a podocyte-like cell with a filtration slit diaphragm, Nature, 2009, vol. 457, pp. 322–326.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Denholm, B. and Skaer, H., Bringing together components of the fly renal system, Curr. Opin. Genet. Dev., 2009, vol. 19, pp. 526–532.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Cagan, R.L., The Drosophila nephrocyte, Curr. Opin. Nephrol. Hypertens., 2011, vol. 20, pp. 409–415.

    Article  PubMed  Google Scholar 

  5. Zhuang, Sh., Shao, H., Guo, F., et al., Sns and Kirre, the Drosophila orthologs of Nephrin and Neph1, direct adhesion, fusion and formation of a slit diaphragm-like structure in insect nephrocytes, Development, 2009, vol. 136, pp. 2335–2344.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Tiwari, S., Riazi, Sh., and Ecelbarger, C.A., Insulin’s impact on renal sodium transport and blood pressure in health, obesity, and diabetes, Am. J. Physiol. Renal Physiol., 2007, vol. 293, pp. F974–F984.

    Article  CAS  PubMed  Google Scholar 

  7. Iglesias, P. and Dez, J.J., Insulin therapy in renal disease, Diabetes Obes. Metab., 2008, vol. 10, pp. 811–823.

    Article  CAS  PubMed  Google Scholar 

  8. Catena, C., Cavarape, A., Novello, M., et al., Insulin receptors and renal sodium handling in hypertensive fructose-fed rats, Kidney Int., 2003, vol. 64, pp. 2163–2171.

    Article  CAS  PubMed  Google Scholar 

  9. Coward, R.J.M., Welsh, G.I., Yang, J., et al., The human glomerular podocyte is a novel target for insulin action, Diabetes Obes. Metab., 2005, vol. 54, pp. 3095–3102.

    CAS  Google Scholar 

  10. Welsh, G.I., Hale, L.J., Eremina, V., et al., Insulin signaling to the glomerular podocyte is critical for normal kidney function, Cell Metab., 2010, vol. 12, pp. 329–340.

    Article  CAS  PubMed  Google Scholar 

  11. Toivonen, J.M. and Partridge, L., Endocrine regulation of aging and reproduction in Drosophila, Mol. Cell. Endocrinol., 2009, vol. 299, pp. 39–50.

    Article  CAS  PubMed  Google Scholar 

  12. Belgacem, Y.H. and Martin, J.-R., Hmgcr in the corpus allatum controls sexual dimorphism of locomotor activity and body size via the insulin pathway in Drosophila, PLoS One, 2007, vol. 2, no. 1. e187. doi 10.1371/journal.pone.0000187

    Article  PubMed Central  PubMed  Google Scholar 

  13. Gruntenko, N., Laukhina, O.V., Bogomolova, E.V., et al., Downregulation of the dopamine 2-like receptor in corpus allatum affects juvenile hormone metabolism in Drosophila melanogaster females, J. Insect Physiol., 2012, vol. 58, pp. 348–355.

    Article  CAS  Google Scholar 

  14. Andreenkova, O.V., Karpova, E.K., and Gruntenko, N.E., Probable role of dopamine in the regulation of ventral nephrocyte functioning in Drosophila melanogaster adults, Physiol. Entomol., 2014, vol. 39, pp. 28–32.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    O. V. Andreenkova, E. K. Karpova, P. N. Menshanov & I. Yu. Rauschenbach

Authors
  1. O. V. Andreenkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. K. Karpova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. N. Menshanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Yu. Rauschenbach
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. V. Andreenkova.

Additional information

Original Russian Text © O.V. Andreenkova, E.K. Karpova, P.N. Menshanov, I.Yu. Rauschenbach, 2015, published in Genetika, 2015, Vol. 51, No. 2, pp. 263–265.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Andreenkova, O.V., Karpova, E.K., Menshanov, P.N. et al. Knockdown of InR gene in ventral nephrocytes promotes resistance to toxic stress in Drosophila melanogaster females. Russ J Genet 51, 210–213 (2015). https://doi.org/10.1134/S1022795415020027

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795415020027

Keywords

Search

Navigation