Russian Journal of Genetics

, Volume 50, Issue 7, pp 757–762 | Cite as

Identification of European (Capreolus capreolus L.) and Siberian (C. pygargus Pall.) roe deer hybrids by microsatellite marker analysis

  • D. A. PlakhinaEmail author
  • E. Yu. Zvychaynaya
  • M. V. Kholodova
  • A. A. Danilkin
Animal Genetics


An analysis of 130 tissue specimens of the European (Capreolus capreolus) and the Siberian (C. pygargus) roe deer from nine regions of Russia and Ukraine using microsatellite loci analysis was conducted, aimed at the identification of hybrid animals. An optimized complex of 21 microsatellite loci was selected for the determination of species specificity and the search for interspecies hybrids. Hybrid animals were revealed in the Moscow and Volgograd regions. Their ratio in the total sample obtained in the European part of Russia was 11.9%.


Rangifer Tarandus Microsatellite Marker Analysis Game Warden Hybrid Animal Volgograd Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Danilkin, A.A., Mlekopitayushchie Rossii i sopredel’nykh regionov: Olen’i (Cervidae) (Mammals of Russia and Neighboring Regions: Deers (Cervidae)), Moscow: GEOS, 1999.Google Scholar
  2. 2.
    Zvychainaya, E.Yu., Kholodova, M.V., and Danilkin, A.A., Consequences of the Siberian roe deer (Capreolus pygargus) dissemination in Eastern Europe: Molecular genetic analysis of the mtDNA control region, in Tselostnost’ vida u mlekopitayushchikh: izoliruyushchie bar’ery i gibridizatsiya (Integrity of Mammalian Species: Isolation Barriers and Hybridization), Moscow: KMK, 2010, p. 39.Google Scholar
  3. 3.
    Zvychainaya, E.Yu., Danilkin, A.A., Sipko, T.P., et al., Genetic diversity of the Siberian roe deer (Capreolus pygargus): mtDNA analysis, in Sokhranenie raznoobraziya zhivotnykh i okhotnich’e khozyaistvo Rossii (Animal Diversity Maintenance and the Game Husbandry in Russia), (Proc. Conf.), 2011, pp. 267–269.Google Scholar
  4. 4.
    Zvychainaya, E.Yu., Kir’yakulov, V.M., Kholodova, M.V., and Danilkin, A.A., Roe deer (Capreolus) gene pool of Moscow Area: analysis of the mtDNA control region variation, Vestn. Okhotoved., 2011, vol. 8, no. 2, pp. 168–173.Google Scholar
  5. 5.
    Danilkin, A.A., Zvychainaya, E.Yu., and Kholodova, M.V., What roe deer inhabits the Volga region?, Vestn. Okhotoved., 2012, vol. 9, no. 2, pp. 200–208.Google Scholar
  6. 6.
    Rossiiskie okhotnich’i trofei: kniga rekordov 2009 (Russian Hunting Trophies: Book of Records 2009), Moscow: Mosk. okhotnichii klub “Safari”, 2010.Google Scholar
  7. 7.
    Danilkin, A.A., Dikie kopytnye v okhotnich’em khozyaistve (osnovy upravleniya resursami) (Wild Ungulates in Hunting (Basic Resource Management)), Moscow: GEOS, 2006.Google Scholar
  8. 8.
    Danilkin, A.A., Biologicheskie osnovy okhotnich’ego trofeinogo dela (The Biological Basis of the Hunting Trophy Business), Moscow: KMK, 2010.Google Scholar
  9. 9.
    Randi, E., Pierpaoli, M., and Danilkin, A., Mitochondrial DNA polymorphism in populations of Siberian and European roe deer (Capreolus pygargus and C. capreolus), Heredity, 1998, vol. 80, no. 4, pp. 429–437.PubMedCrossRefGoogle Scholar
  10. 10.
    Tokarskaya, O.N., Efremova, D.A., Kan, N.G., et al., Variability of multilocus DNA markers in populations of Siberian (Capreolus pygargus Pall.) and European (C. capreolus L.) roe deer, Russ. J. Genet., 2000, vol. 36, no. 11, pp. 1278–1287.Google Scholar
  11. 11.
    Petrosyan, V.G., Tokarskaya, O.N., Danilkin, A.A., and Ryskov, A.P., Quantitative analysis of genetic parameters in populations of European (Capreolus capreolus L.) and Siberian (Capreolus pygargus Pall.) roe deer with RAPD markers, Russ. J. Genet., 2002, vol. 38, no. 6, pp. 676–683.CrossRefGoogle Scholar
  12. 12.
    Zvychaynaya, E.Yu., Volokh, A.M., Kholodova, M.V., and Danilkin, A.A., Mitochondrial DNA polymorphism of the European roe deer, Capreolus capreolus (Artiodactyla, Cervidae), from the South-West of Ukraine, Vestn. Zool., 2013, vol. 47, no. 5, pp. 415–420.Google Scholar
  13. 13.
    Wilson, G.A., Strobeck, C., Wu, L., and Coffin, J.W., Characterization of microsatellite loci in caribou, Rangifer tarandus, and their use in other Artiodactyls, Mol. Ecol., 1997, vol. 6, no. 7, pp. 697–699.PubMedCrossRefGoogle Scholar
  14. 14.
    Kappes, S.M., Keele, J.W., Stone, R.T., et al., A second-generation linkage map of the bovine genome, Genome Res., 1997, vol. 7, no. 3, pp. 235–249.PubMedCrossRefGoogle Scholar
  15. 15.
    Moore, S.S., Byrne, K., and Berger, K.T., Characterization of 65 bovine microsatellites, Mamm. Genome, 1994, vol. 5, no. 2, p. 84–90.PubMedCrossRefGoogle Scholar
  16. 16.
    Buchanan, F.C. and Crawford, A.M., Ovine microsatellites at the OarFCB11, OarFCB128, OarFCB193, OarFCB266 and OarFCB304 loci, Anim. Genet., 1993, vol. 24, no. 2, p. 145.PubMedCrossRefGoogle Scholar
  17. 17.
    Stone, R.T., Pulido, J.C., Duyk, G.M., et al., A small-insert bovine genomic library highly enriched for microsatellite repeat sequences, Mamm. Genome, 1995, vol. 6, p. 714.PubMedCrossRefGoogle Scholar
  18. 18.
    Jobin, R.M., Patterson, D., and Zhang, Y., DNA typing in populations of mule deer for forensic use in the province of Alberta, Genetics, 2008, vol. 2, no. 3, pp. 190–197.Google Scholar
  19. 19.
    Bishop, M.D., Kappes, S.M., Keele, J.W., et al., A genetic linkage map for cattle, Genetics, 1994, vol. 136, pp. 619–639.PubMedCentralPubMedGoogle Scholar
  20. 20.
    Roed, K.H. and Midthjell, L., Microsatellites in reindeer, Rangifer tarandus, and their use in other cervids, Mol. Ecol., 1998, vol. 7, no. 12, pp. 1773–1776.Google Scholar
  21. 21.
    Park, S.D.E., Trypanotolerance in Western African cattle and the population genetic effects of selection, Ph.D., Dublin: University of Dublin, 2001.Google Scholar
  22. 22.
    Peakall, R. and Smouse, P.E., GenAlEx 6: genetic analysis in Excel: population genetic software for teaching and research, Mol. Ecol., 2006, vol. 6, no. 1, pp. 288–295.CrossRefGoogle Scholar
  23. 23.
    Falush, D., Stephens, M., and Pritchard, J.K., Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies, Genetics, 2003, vol. 164, no. 4, pp. 1567–1587.PubMedCentralPubMedGoogle Scholar
  24. 24.
    Pavlov, M.P., Akklimatizatsiya okhotnich’e-promyslovykh zverei i ptits v SSSR (Acclimatization of Commercial Game Mammals and Birds in the Soviet Union), Kirov, 1999.Google Scholar
  25. 25.
    Pavlov, M.P., Korsakova, I.B., and Lavrov, N.P., Akklimatizatsiya okhotnich’e-promyslovykh zverei i ptits v SSSR (Acclimatization of Commercial Game Mammals and Birds in the Soviet Union), Kirov, 1974.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2014

Authors and Affiliations

  • D. A. Plakhina
    • 1
    Email author
  • E. Yu. Zvychaynaya
    • 1
  • M. V. Kholodova
    • 1
  • A. A. Danilkin
    • 1
  1. 1.Severtsov Institute of Ecology and Evolution ProblemsRussian Academy of SciencesMoscowRussia

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