Abstract
In this study, we compare the variability of the regulatory region of the Lim3 gene, which plays a key role in the development of the nervous system, in two populations of Drosophila melanogaster inhabiting the cities of Aleksandrov (Russia) and Raleigh (United States). The two population areas are located in different geographic regions and differ in their ecology. A comparison of nucleotide sequences of 16 (2010) and 20 (2011) alleles from the Alexandrov population showed that in both cases the variability level of the untranslated Lim3 region was considerably lower than that of the 5’ regulatory region adjacent to the transcription start site. According to quantitative and qualitative parameters of the variability, there was no difference between samples of different years, which indicates the stability of the population inhabiting the northern border of the species areal. The patterns of polymorphic sites are similar in both populations, which suggest a neutral character of the variability found with respect to environmental factors, as well as the importance of nucleotide substitutions in a number of sites of the Lim3 regulatory region with respect to the control of this gene expression.
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Moriyama, E.N. and Powell, J.R., Intraspecific nuclear DNA variation in Drosophila, Mol. Biol. Evol., 1996, vol. 13, pp. 261–277.
De Luca, M., Roshina, N.V., Geiger-Thornsberry, G.L., et al., Dopa decarboxylase (Ddc) affects variation in Drosophila longevity, Nat. Genet., 2003, vol. 34, pp. 429–433.
Palsson, A., Rouse, A., Riley-Berger, R., et al., Nucleotide variation in the Egfr locus of Drosophila melanogaster, Genetics, 2004, vol. 167, pp. 1199–1212.
Simonenko, A.V., Rybina, O.Yu., and Pasyukova, E.G., Molecular variation of the shuttle craft and Lim3 genes, controlling the development of the nervous system, in a natural Drosophila melanogaster population, Russ. J. Genet., 2008, vol. 44, no. 9, pp. 1020–1024.
Rybina, O.Y. and Pasyukova, E.G., A naturally occurring polymorphism at Drosophila melanogaster Lim3 locus, a homolog of human LHX3/4, ffects Lim3 transcription and fly lifespan, PLoS One, 2010, vol. 5. e12651.
Sackton, T.B., Kulathinal, R.J., Bergman, C.M., et al., Population genomic inferences from sparse high-throughput sequencing of two populations of Drosophila melanogaster, Genome Biol. Evol., 2009, vol. 1, pp. 449–465.
Langley, C.H., Stevens, K., Cardeno, C., et al., Genomic variation in natural populations of Drosophila melanogaster, Genetics, 2012, vol. 192, pp. 533–598.
Mackay, T.F., Richards, S., Stone, E.A., et al., The Drosophila melanogaster genetic reference panel, Nature, 2012, vol. 482, pp. 173–178.
Frydenberg, J., Hoffmann, A.A., and Loeschcke, V., DNA sequence variation and latitudinal associations in hsp23, hsp26 and hsp27 from natural populations of Drosophila melanogaster, Mol. Ecol., 2003, vol. 12, pp. 2025–2032.
Paaby, A.B., Blacket, M.J., Hoffmann, A.A., and Schmidt, P.S., Identification of a candidate adaptive polymorphism for Drosophila life history by parallel independent clines on two continents, Mol. Ecol., 2010, vol. 19, pp. 760–774.
Kolaczkowski, B., Kern, A.D., Holloway, A.K., and Begun, D.J., Genomic differentiation between temperate and tropical Australian populations of Drosophila melanogaster, Genetics, 2011, vol. 187, pp. 245–260.
Fabian, D.K., Kapun, M., Nolte, V., et al., Genome-wide patterns of latitudinal differentiation among populations of Drosophila melanogaster from North America, Mol. Ecol., 2012, vol. 21, pp. 4748–4769.
Thor, S., Andersson, S.G., Tomlinson, A., and Thomas, J.B., A LIM-homeodomain combinatorial code for motor-neuron pathway selection, Nature, 1999, vol. 397, pp. 76–80.
Varela-Echavarría, A., Pfaff, S.L., and Guthrie, S., Differential expression of LIM homeobox genes among motor neuron subpopulations in the developing chick brain stem, Mol. Cell Neurosci., 1996, vol. 8, pp. 242–257.
Sharma, K., Sheng, H.Z., Lettieri, K., et al., LIM homeodomain factors Lhx3 and Lhx4 assign subtype identities for motor neurons, Cell, 1998, vol. 95, pp. 817–828.
Barker, J.S.F., Sexual isolation between Drosophila melanogaster and Drosophila simulans, Am. Nat., 1962, vol. 96, pp. 105–115.
Tajima, F., Statistical method for testing the neutral mutation hypothesis by DNA polymorphism, Genetics, 1989, vol. 123, pp. 585–595.
Fu, Y.X. and Li, W.H., Statistical tests of neutrality of mutations, Genetics, 1993, vol. 133, pp. 693–709.
Librado, P. and Rozas, J., DnaSP v. 5: a software for comprehensive analysis of DNA polymorphism data, Bioinformatics, 2009, vol. 25, pp. 1451–1452.
Demerec, M., The Biology of Drosophila, Cold Spring Harbour: CSHL Press, 1994.
Andolfatto, P., Adaptive evolution of non-coding DNA in Drosophila, Nature, 2005, vol. 437, pp. 1149–1152.
Urbach, R. and Technau, G.M., Molecular markers for identified neuroblasts in the developing brain of Drosophila, Development, 2003, vol. 130, pp. 3621–3637.
Li, X., Erclik, T., Bertet, C., et al., Temporal patterning of Drosophila medulla neuroblasts controls neural fates, Nature, 2013, vol. 498, pp. 456–462.
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Original Russian Text © E.R. Veselkina, O.Yu. Rybina, A.V. Symonenko, V.E. Alatortsev, N.V. Roshchina, E.G. Pasyukova, 2014, published in Genetika, 2014, Vol. 50, No. 6, pp. 629–637.
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Veselkina, E.R., Rybina, O.Y., Symonenko, A.V. et al. Molecular variability in geographically distant populations of Drosophila melanogaster at the Lim3 gene regulating nervous system development. Russ J Genet 50, 549–556 (2014). https://doi.org/10.1134/S1022795414050111
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DOI: https://doi.org/10.1134/S1022795414050111