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Sin3 histone deacetylase controls level of spontaneous and UV-induced mutagenesis in yeast Saccharomyces cerevisiae

  • Genetics of Microorganisms
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Abstract

SIN3 gene product operates as a repressor for a huge amount of genes in Saccharomyces cerevisiae. Sin3 protein with a mass of about 175 kDa is a member of the RPD3 protein complex with an assessed mass of greater than 2 million Da. It was previously shown that RPD3 gene mutations influence recombination and repair processes in S. cerevisiae yeasts. We studied the impacts of the sin3 mutation on UV-light sensitivity and UV-induced mutagenesis in budding yeast cells. The deletion of the SIN3 gene causes weak UV-sensitivity of mutant budding cells as compared to the wild-type strain. These results show that the sin3 mutation decreases both spontaneous and UV-induced levels of levels. This fact is hypothetically related to the malfunction of ribonucleotide reductase activity regulation, which leads to a decrease in the dNTP pool and the inaccurate error-prone damage bypass postreplication repair pathway, which in turn provokes a reduction in the incidence of mutations.

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Authors and Affiliations

  1. Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russia

    I. Yu. Lebovka, T. N. Kozhina, I. V. Fedorova, V. T. Peshekhonov, T. A. Evstyukhina, A. Yu. Chernenkov & V. G. Korolev

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  1. I. Yu. Lebovka
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  2. T. N. Kozhina
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  3. I. V. Fedorova
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  4. V. T. Peshekhonov
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  5. T. A. Evstyukhina
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  6. A. Yu. Chernenkov
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  7. V. G. Korolev
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Original Russian Text © I.Yu. Lebovka, T.N. Kozhina, I.V. Fedorova, V.T. Peshekhonov, T.A. Evstyukhina, A.Yu. Chernenkov, V.G. Korolev, 2014, published in Genetika, 2014, Vol. 50, No. 1, pp. 5–11.

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Lebovka, I.Y., Kozhina, T.N., Fedorova, I.V. et al. Sin3 histone deacetylase controls level of spontaneous and UV-induced mutagenesis in yeast Saccharomyces cerevisiae . Russ J Genet 50, 1–6 (2014). https://doi.org/10.1134/S1022795413110124

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  • DOI: https://doi.org/10.1134/S1022795413110124

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