Russian Journal of Genetics

, Volume 49, Issue 10, pp 1004–1012 | Cite as

Interactions of [NSI +] prion-like determinant with SUP35 and VTS1 genes in Saccharomyces cerevisiae

  • A. A. Nizhnikov
  • A. M. Kondrashkina
  • A. P. Galkin
Genetics of Microorganisms

Abstract

Previously we characterized [NSI +], determinant, that possesses the features of a yeast prion. This determinant causes the nonsense suppression in strains that bear different N-substituted variants of Sup35p, which is a translation release factor eRF3. As a result of the genomic screen, we identified VTS1, the overexpression of which is a phenotypic copy of [NSI +]. Here, we analyzed the influence of SUP35 and VTS1 on [NSI +]. We demonstrated nonsense suppression in the [NSI +] strains, which appears when SUP35 expression was decreased or against a background of general defects in the fidelity of translation termination. [NSI +] has also been shown to increase VTS1 mRNA amounts. These findings facilitate the insight into the mechanisms of nonsense suppression in the [NSI +] strains and narrow the range of candidates for [NSI +] determinant.

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Copyright information

© Pleiades Publishing, Inc. 2013

Authors and Affiliations

  • A. A. Nizhnikov
    • 1
    • 2
  • A. M. Kondrashkina
    • 2
  • A. P. Galkin
    • 1
    • 2
  1. 1.Vavilov Institute of General GeneticsRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Department of Genetics and BiotechnologySt. Petersburg State UniversitySt. PetersburgRussia

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