Abstract
The effect of the ubiquitous downregulation of insulin receptor (InR) gene expression on the metabolism of juvenile hormone (JH) and dopamine (DA) in young females of D. melanogaster under normal conditions and heat stress is studied. The level of JH degradation and alkaline phosphatase activity (ALP, an enzyme regulating DA synthesis) were used as indicators of JH and DA levels, respectively. We demonstrated that, under normal conditions, the ubiquitous inhibition of the InR gene expression in D. melanogaster females induced an increase in the JH degradation and ALP activity. As we have already shown, this is indicative of the decrease in the concentration of the above hormones. It was also found that the total inactivation of InR does not affect the initiation of JH and DA metabolic system response to heat stress; however, it does affect its intensity. Thus, the involvement of the insulin signaling pathway in the regulation of the JH and DA metabolism in Drosophila females was demonstrated in vivo under normal and stress conditions.
References
Gruntenko, N.E. and Rauschenbach, I.Yu., Interplay of juvenile hormone, 20-hydroxyecdisone and biogenic amines under normal and stress conditions and its effect on reproduction, J. Insect Physiol., 2008, vol. 54, pp. 902–908.
Gruntenko, N., Wen, D., Karpova, E.K., et al., Altered juvenile hormone metabolism, reproduction and stress response in Drosophila adults with genetic ablation of the corpus allatum cells, Insect Biochem. Mol. Biol., 2010, vol. 40, pp. 891–897.
Gruntenko, N., Bogomolova, E.V., Adonyeva, N.V., et al., Decrease in juvenile hormone level as a result of genetic ablation of the corpus allatum cells affects the synthesis and metabolism of stress related hormones in Drosophila, J. Insect Physiol., 2012, vol. 58, pp. 49–55.
Rauschenbach, I.Yu., Bogomolova, E.V., Karpova, E.K., et al., Mechanisms of age-specific regulation of dopamine metabolism by juvenile hormone and 20-hydroxyecdysone in Drosophila females, J. Comp. Physiol. B, 2011, vol. 181, pp. 19–26.
Gruntenko, N., Laukhina, O.V., Bogomolova, E.V., et al., Downregulation of the dopamine 2-like receptor in corpus allatum affects juvenile hormone metabolism in Drosophila melanogaster females, J. Insect Physiol., 2012, vol. 58, pp. 348–355.
Tatar, M., Bartke, A., and Antebi, A., The endocrine regulation of aging by insulin-like signals, Science, 2003, vol. 299, pp. 1346–1351.
Broughton, S.J., Piper, M.D., Ikeya, T., et al., Longer lifespan, altered metabolism, and stress resistance in Drosophila from ablation of cells making insulin-like ligands, Proc. Natl. Acad. Sci. U.S.A., 2005, vol. 102, pp. 3105–3110.
Piper, M.D., Selman, C., McElwee, J.J., and Partridge, L., Separating cause from effect: How does insulin/IGF signalling control lifespan in worms, flies and mice?, J. Int. Med., 2008, vol. 263, pp. 179–191.
Gulia-Nuss, M., Robertson, A.E., Brown, M.R., and Strand, M.R., Insulin-like peptides and the target of rapamycin pathway coordinately regulate blood digestion and egg maturation in the mosquito Aedes aegypti, PLoS One, 2011, vol. 6, no. 5, p. e20401. doi:10.1371/journal.pone.0020401
Toivonen, J.M. and Partridge, L., Endocrine regulation of aging and reproduction in Drosophila, Mol. Cell. Endocrinol., 2009, vol. 299, pp. 39–50.
Belgacem, Y.H. and Martin, J-R., Hmgcr in the corpus allatum controls sexual dimorphism of locomotor activity and body size via the insulin pathway in Drosophila, PLoS One, 2007, vol. 2, no. 1, p. e187. doi: 10.1371/journal.pone.0000187
Tu, M.-P., Yin, C.-M., and Tatar, M., Mutations in insulin signaling pathway alter juvenile hormone synthesis in Drosophila melanogaster, Gen. Comp. Endocrinol., 2005, vol. 142, pp. 347–356.
Draper, I., Kurshan, P.T., McBride, E., et al., Activity is regulated by D2-like receptors in Drosophila: An anatomic and functional analysis, Dev. Neurobiol., 2007, vol. 67, pp. 378–393.
Bogomolova, E.V., Rauschenbach, I.Yu., Adonyeva, N.V., et al., Dopamine down-regulates activity of alkaline phosphatase in Drosophila: The role of D2-like receptors, J. Insect Physiol., 2010, vol. 56, pp. 1155–1159.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.V. Bogomolova, N.V. Adonyeva, E.K. Karpova, N.E. Gruntenko, I.Yu. Rauschenbach, 2013, published in Genetika, 2013, Vol. 49, No. 7, pp. 891–895.
Rights and permissions
About this article
Cite this article
Bogomolova, E.V., Adonyeva, N.V., Karpova, E.K. et al. Ubiquitous downregulation of InR gene expression affects stress associated hormone metabolism in Drosophila females. Russ J Genet 49, 778–781 (2013). https://doi.org/10.1134/S1022795413050037
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795413050037