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Characterization of puroindolines in the control of endosperm texture in common wheat lines with substitutions of homeologous group-5 chromosomes

  • Plant Genetics
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Abstract

The genetic control of grain hardness and its association with the specific friabilin content on starch granules of common wheat cultivars and lines with intervarietal substitutions of homeologous group-5 chromosomes were studied. A significant correlation was revealed between the technological parameters of grain hardness (mean size of flour particles) and the specific content of puroindolines on the starch surface estimated in terms of starch doses. The results obtained allowed the method of starch doses to be used to identify soft and hard wheat cultivars and lines based on an analysis of a single grain. The biochemical analysis confirmed the previously obtained estimates of flour-grinding properties of wheat cultivars and substitution lines and allowed specific genotypes to be characterized according to the composition of puroindolines. The influence of chromosomes 5D and 5A of donor wheat cultivars on the activity of the Ha loci of recipient cultivars was revealed and found to be associated with the composition of PIN products and with the expression of the Pina-D1 and Pinb-D1 genes.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    L. V. Obukhova, T. T. Efremova & V. K. Shumny

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  1. L. V. Obukhova
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  2. T. T. Efremova
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  3. V. K. Shumny
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Correspondence to L. V. Obukhova.

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Dedicated to the memory of M.F. Ermakova

Original Russian Text © L.V. Obukhova, T.T. Efremova, V.K. Shumny, 2013, published in Genetika, 2013, Vol. 49, No. 3, pp. 337–342.

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Obukhova, L.V., Efremova, T.T. & Shumny, V.K. Characterization of puroindolines in the control of endosperm texture in common wheat lines with substitutions of homeologous group-5 chromosomes. Russ J Genet 49, 294–298 (2013). https://doi.org/10.1134/S1022795412120071

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  • DOI: https://doi.org/10.1134/S1022795412120071

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